1. Institute of Industrial Ecology and Environment, Zhejiang University, Hangzhou 310027, China 2. The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310007, China
A repetitive high-voltage nanosecond pulsed electric field (RnsPEF) generation system was independently developed based on the spark switch and transmission line transformer (TLT) technology in order to analyze the key impact parameters of the process of malignant tumors ablation by high-voltage nanosecond pulsed electric field (nsPEF). The system can stably generate nanosecond exponential pulse. The experimental results proved the effectivity and controllability of RnsPEF on tumor cells ablation. B16 melanoma cells adherently seeded in six-well plates as the research object to analyze the effects of pulse number, peak voltage, repetition frequency and electrode spacing on tumor cells ablation. Cell counting kit-8 (CCK-8) was applied to measure cell viability of B16 tumor cells suspension in the cuvette after treated by pulses. The experimental results show that the pulsed electric field intensity and injected energy density of the applied RnsPEF play the key roles in determining the ablation effect. The repetition frequency hardly affects the ablation results. The pulsed electric field intensity threshold of RnsPEF ablating B16 melanoma cells is 6.8 kV/cm, and the injected energy density threshold is 11.4 J/cm3, as well as the optimal pulse number is 500 pulses.
Zhen-hong MA,Zhen LIU,Sheng-yong YIN,Rong-wei MA,Ke-ping YAN. Experimental study on melanoma cell ablation by high-voltage nanosecond pulsed electric field. Journal of ZheJiang University (Engineering Science), 2021, 55(6): 1168-1174.
Fig.1Experimental flow chart for B16 tumor cells ablation in vitro
Fig.2RnsPEF generation system
Fig.3Profile of spark switch
Fig.4Schematic diagram of transmission line transformer
Fig.5Electrodes and cuvette for pulse processing
Fig.6Voltage and current waveforms
Fig.7Ablation effect of RnsPEF system under different pulse numbers (repetition frequency is 10 pps, electrodes E[0.5,10,10])
Fig.8Ablation area of RnsPEF system under different pulse numbers (repetition frequency is 10 pps, electrodes E[0.5,10,10])
Fig.9Ablation area of RnsPEF system under different peak voltages (repetition frequency is 10 pps, pulse number is 500 pulses, electrodes E[0.5,10,10])
Fig.10Ablation effect of RnsPEF system under different repetition frequency (peak voltage is 26 kV, pulse number is 500 pulses, electrodes E[1.0,12,10])
Fig.11Ablation effect of RnsPEF system under electrodes with different spacings (repetition frequency is 10 pps, peak voltage is 26 kV, pulse number is 500 pulses)
Fig.12Ablation area of RnsPEF system under electrodes with different spacings (repetition frequency is 10 pps, peak voltage is 26 kV, pulse number is 500 pulses)
Fig.13Temperature distribution of culture medium after pulse treatment (repetition frequency is 10 pps, peak voltage is 28 kV, pulse number is 800 pulses)
Fig.14Cell viability of B16 tumor cells under different electric field intensity (repetition frequency is 10 pps, pulse number is 500 pulses)
Fig.15Cell viability of B16 tumor cells under different pulse number (repetition frequency is 10 pps, electric field intensity is 6.8 kV/cm)
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