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Application and market of agricultural sensor technology in China: current status and future perspectives
Yufei LIU,Yong HE,Fei LIU,Lijia XU,Xuping FENG,Yu TANG,Zhengxiao WANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 293-304.   DOI: 10.3785/j.issn.1008-9209.2022.04.062
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Sensor technology is the foundation of agricultural informatization, and it is one of the core elements and key support to realize agricultural modernization. First, this paper summarizes the current technical status and application of agricultural sensor technology in three areas of intelligent agricultural machinery equipment, agricultural unmanned aerial vehicle (UAV) based remote sensing, and agricultural internet of things, and conducts an in-depth analysis on the technical demands and market development of agricultural sensor in China. Second, the industrialization, marketization, and future development trends of agricultural sensors were summarized and pointed out through the technical industry analysis. Finally, 16 key technologies in the field of agricultural sensor industry were condensed, and on this basis, a Delphi-based expert questionnaire survey was carried out. The results showed that universality was the most important attribute of agricultural sensors; basic theory and research and development input were the two biggest constraints to the development of agricultural sensor technology. It was proposed that the agricultural sensor technology would develop towards low cost, high stability, high intelligence, portability, and operability in the future. This paper provides a reference for the technical and industrial development of agricultural sensors in China.

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Research advances in the mechanisms of protecting animals against influenza by probiotics
Meiqing HAN,Di WANG,Xianqi PENG,Yan LI
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (6): 755-764.   DOI: 10.3785/j.issn.1008-9209.2022.07.182
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Influenza is an important zoonotic disease. The highly pathogenic avian influenza not only brings enormous losses to China’s livestock breeding industry but also seriously threatens the safety of public health. The ability of influenza viruses to recombine in swine and spread across species barriers poses significant challenges for influenza prevention. Due to the rapid mutation of influenza viruses, the differences between prevalent viruses and vaccine strains reduce the vaccine efficacy. It is necessary to improve the host’s immunity to influenza viruses. Probiotics regulate the balance of intestinal microbiota and promote body health, which is beneficial for protecting animal against influenza viruses. This paper reviewed the mechanisms of anti-influenza virus action of probiotics in animals. The mechanisms include direct or indirect interference with virus attacks by balancing the composition of intestinal flora, regulating the mucosal barrier function of body, and enhancing or suppressing Toll-like receptor-related molecular signaling pathways. This study provides scientific evidence for understanding the mechanisms by which different strains of probiotics combat influenza and for developing more effective anti-influenza strategies.

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Research progresses on target of rapamycin kinase in plants
Wenzhen CHEN,Jiaqi LIU,Hao DU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 591-606.   DOI: 10.3785/j.issn.1008-9209.2023.05.101
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In plants, target of rapamycin (TOR) functions as a pivotal signaling and metabolic hub, integrating nutrient availability, energy status, and environmental cues through phosphorylation. This regulatory mechanism plays a crucial role in governing plant growth, development, and environmental adaptation. In this paper, we provide a comprehensive review of the discovery and characterization of TOR in plants. We summarize previous and recent studies on the signaling pathway of plant TOR, highlighting the identification of upstream effect factors and downstream substrates. Additionally, we discuss the diverse roles of TOR in plant embryogenesis, meristem formation, nutrient utilization, flowering, senescence, and responses to both abiotic and biotic stresses. Furthermore, we explore the potential research prospects for TOR kinase and its application in agriculture.

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Research progress on the regulation of vascular lignification on defense against bacterial wilt of plants
Chenying LI,Ran WANG,Yan LIANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 633-643.   DOI: 10.3785/j.issn.1008-9209.2023.06.171
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Bacterial wilt, a typical vascular disease caused by Ralstonia solanacearum, is one of the most devastating diseases and dramatically reduces crop yield and quality. The most effective strategy for controlling wilt disease is breeding disease-resistant varieties, which requires understanding the molecular mechanisms underlying plant immune responses against R. solanacearum. However, more and more evidence suggests that vascular immune responses are cell type specific. After sensing of R. solanacearum, the cell wall lignification of vascular tissues plays a vital role in restricting the spread of R. solanacearum. Lignin biosynthesis pathway genes are strictly controlled at the transcriptional, translational, and spatial-temporal specific expression aspects. Here, we summarized the current understanding of the recognition and signal transductionupon R. solanacearum infection and the research progress of pathogen-induced vascular lignification on regulating resistance to R. solanacearum, including the expression of lignin biosynthesis genes, the transport and polymerization of monolignols, and the generation of different types of lignin. We hope that this review will provide a theoretical basis for breeding bacterial wilt disease-resistant cultivars by modifying vascular lignification.

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Entire issue
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (4): 445-590.  
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Research progress on the molecular basis of plant-Ralstonia solanacearum recognition
Zhiliang XIAO,Aiguo YANG,Meixiang ZHANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 644-650.   DOI: 10.3785/j.issn.1008-9209.2023.06.161
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Ralstonia solanacearum causes bacterial wilt disease in multiple crops, which severely threatens the global crop safety production. This pathogen exhibits high genetic diversity and evolves rapidly, and there is a lack of effective disease-resistant varieties in production, which brings great challenges for effective disease control. Identifying receptor proteins in plants that recognize associated molecular patterns or effectors of R. solanacearum and elucidating their molecular recognition mechanisms can provide clues to understand the mechanisms of plant-pathogen interaction, and lay a basis for the development of broad-spectrum disease resistance in plants. This paper reviewed the recent progress on the molecular basis of plant-R. solanacearum recognition. We mainly focused on the identification and functional analysis of membrane and intracellular receptors that recognize R. solanacearum in plants, as well as the mechanism behind receptor recognition of the associated molecular patterns or effectors from R. solanacearum. Besides, we provide research prospects for the exploration and utilization of disease-resistant resources against R. solanacearum in the future.

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Entire issue
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (6): 1-902.  
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Entire issue
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 591-754.  
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Molecular tools and technological innovation in oil crop breeding
Ling XU,Hui LIU,Guijun YAN,Wallace COWLING,Weijun ZHOU,Zhanyuan LU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (4): 445-453.   DOI: 10.3785/j.issn.1008-9209.2023.04.181
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Oil crop breeding programs generally aim to improve yield, quality, and stress resistance. Major oil crops include soybean, rape, sunflower and peanut according to their current production worldwide. This paper reviewed the molecular tools and technological innovation in oil crop breeding, including advanced technologies such as genome selection, genome editing, and molecular design breeding. Challenges exist in current genetic studies and breeding practices, and future perspectives of technological progress and application are also discussed for achieving high yield, high quality, and efficient breeding of oil crops.

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Bioinformatics and expression analyses of heat shock protein 90 gene family in Rhododendron ovatum
Fengyan LI,Fangqian QU,Fangmeng ZHAO,Qi WANG,Hong ZHOU,Liangsheng ZHANG,Yiping XIA,Xiuyun WANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 708-718.   DOI: 10.3785/j.issn.1008-9209.2022.08.012
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To explore the roles of heat shock protein 90 (Hsp90) in plant growth development and response to high temperature stress, this study identified the Hsp90 family through bioinformatics methods in the whole genome of Rhododendron ovatum,which is a highly ornamental and widely adaptive species of Rhododendron. Gene structure, cis-acting elements, evolution, and expression pattern of the Hsp90 family were subsequently analyzed. The results showed that 11 members of the Hsp90 family were identified in R. ovatum, which were located on five chromosomes. Cis-acting element analysis showed that all the 11 members of the Hsp90 family were involved in the processes of plant hormone metabolism and stress responses. The phylogenetic tree of Hsp90 family in Arabidopsis thaliana, Solanum lycopersicum, Camellia sinensis, R. simsii, R. delavayi, R. henanense, and R. ovatum was constructed, within four main clades. Evolutionary analysis showed that the Hsp90 family has undergone purification selection during the differentiation of Rhododendron species. Moreover, the expression patterns of Hsp90 family in different tissues and under high temperature treatments indicated that Hsp90 family plays roles in flower development and the response of plants to high temperature stress. This study provides a foundation for further study of the functions of Hsp90 genes in Rhododendron.

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Comparison of cell lineage development and regulation during early embryonic development in cattle and mice
Xiaotong WU,Yan SHI,Shuang LI,Shaohua WANG,Kun ZHANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (6): 765-775.   DOI: 10.3785/j.issn.1008-9209.2022.12.131
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Low reproductive efficiency of high-yielding dairy cows is a worldwide challenge, among which the high mortality rate of early embryos is one of the main reasons. Scientists have gained insightful knowledge into early embryonic development in mice while very little work has been performed in cattle. To better understand the early embryonic development in cattle, we compared early embryonic development in cattle and mice from the perspectives of embryo morphology, transcription factors and signaling pathways. It was found that after fertilization, degradation of maternal factors, zygotic genome activation, construction of cell polarity and asymmetric division occurred at different periods in the early embryos of cattle and mice. Eventually, embryos develop to blastocysts with three germ layers (trophectoderm, epiblast and primitive endoderm). Furthermore, multiple transcription factors and signaling pathways form complicated networks to regulate cell lineage differentiation during early embryonic development in cattle and mice. In summary, similar biological events occur one after another at the early embryonic development in cattle and mice, but the regulation of cell lineage differentiation is different, suggesting the limitations of using the early embryo of mouse as a research model. The study in this field plays a crucial role in improving reproductive efficiency of dairy cows and promoting the genetic improvement in cattle.

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Cloning and expression analysis of OsSPL3 promoter in rice
Huiling ZENG,Zuyi MO,Qiaoxian PU,Jiashu WANG,Kai FAN,Zhaowei LI
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 319-327.   DOI: 10.3785/j.issn.1008-9209.2022.05.091
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OsSPL transcription factor plays an important role in the development and stress response of rice (Oryza sativa) roots, leaves, floral organs, and ears. In this study, the OsSPL3 promoter was analyzed to explore the expression pattern of OsSPL3 transcription factor in rice and its response to drought stress. The Cis-acting elements in the OsSPL3 promoter region were analyzed by PLACE and Plant CARE online softwares, and the recombinant expression vector of OsSPL3 promoter and β-glucuronidase(GUS) gene was constructed, which was transformed into ZH11 rice callus, and positive transgenic plants were obtained by screening. The GUS expression activity of pOsSPL3-GUS transgenic plants and the expression patterns under drought stress and abscisic acid (ABA) treatments were detected. The results of promoter analysis showed that in addition to the necessary transcription initiation core elements and light-responsive elements, the OsSPL3 promoter region also included three MYB-involved drought-inducible elements, three gibberellin-responsive elements, two anaerobic induction essential elements, one low temperature response element, one endosperm expression regulatory element, one zein metabolism regulatory element and one meristem expression-related regulatory element. The results of GUS staining showed that the expression activity of GUS gene in young leaves, stem sheaths, coleoptiles and other young tissues was high, as well as in the vigorous growth parts of roots such as root cap, meristem zone, and elongation zone. In addition, the drought stress could significantly enhanced the GUS activity of transgenic rice leaves and roots. It shows that OsSPL3 transcription factor plays a regulatory role in the process of coleoptile growth, new leaf formation, root extension and stem sheath elongation after seed germination, and OsSPL3 transcription factor is also involved in the response process of rice drought stress.

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Isolation and identification of tomato bacterial spot pathogen in Ningxia and screening of identification methods for disease resistance
Xiaohong LI,Xiaomin WANG,Wenxia DENG,Kaibin WANG,Meng GUO,Guoxin CHENG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 349-357.   DOI: 10.3785/j.issn.1008-9209.2022.07.042
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In order to identify the pathogens causing tomato bacterial spot disease in Ningxia, tomato leaves infected with bacterial spot disease from Zhongwei and Wuzhong cities of Ningxia were used as the materials, and the strains were isolated using the conventional tissue isolation method, and their morphology, molecular biology and pathogenicity were identified. The four-factor and three-level orthogonal design of experiment [L9(34)] was conducted to screen the indoor resistance identification method at the seedling stage. The results showed that the colony morphologies of strain 1 isolated from Zhongwei City and strain 2 isolated from Wuzhong City were all milky white, full edge, opaque, smooth surface, producing green fluorescence, rod-shaped cells, and Gram staining was negative. The 16S rDNA sequences of both strain 1 and strain 2 were 99.93% similar to that of Pseudomonas syringae pv. tomato pathogenic variant KT783475.1. After reinoculation, brown lesions with yellow halos were presented on the leaves of the tomato, which was consistent with the natural symptoms. So, it was confirmed that the pathogen causing tomato bacterial spot disease in Ningxia was P. syringae pv. tomato. Under the treatment combination of inoculating seedlings at the four-leaf stage, the bacterial suspension concentration of 1.00×107 CFU/mL, with stem inoculation method and 96 h moisture, the disease index was 72.22%, which was significantly higher than that in other treatments, and thus it was considered to be the best indoor resistance identification method at the seedling stage for tomato bacterial spot disease. The above results will provide a theoretical basis for the prevention and control of tomato bacterial spot disease and resistance breeding in Ningxia.

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Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight
Binhao WANG,Yanbo CHEN,Rongjie LIU,Dongqun WANG,Zuozhen DONG,Rui DI,Xiao WANG,Yuping WU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (6): 840-852.   DOI: 10.3785/j.issn.1008-9209.2023.01.131
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Bacterial leaf blight in rice is a significant disease, and understanding the effects of straw burning on pathogens and antibiotic resistance genes (ARGs) in paddy fields is crucial. Soil and rice stubble samples were collected from bacterial leaf blight outbreak areas in Ningbo City of Zhejiang Province, and these samples were subjected to high-throughput metagenomic sequencing to investigate the effects of straw burning. The results indicated that the relative abundance of pathogens in the rice stubble was significantly higher than that in the soil. After straw burning, except for the significant increase in available potassium content, there were no significant changes in the other physicochemical properties of the soil. Overall, there were no significant differences in pathogen community diversity after straw burning, but straw burning led to a sharp decrease in the relative abundance of Xanthomonas oryzae in the rice stubble. The relative abundances of ARGs in the soil and rice stubble increased after straw burning, but there were no significant differences compared with those before straw burning (P>0.05), while the relative abundance of mobile genetic elements (MGEs) in the rice stubble decreased significantly after straw burning (P<0.05). The proportion of positively correlated links in the coexisting networks of pathogens-ARGs decreased after straw burning. Mantel analysis revealed that the water content (r=0.642, p<0.01), pH value (r=0.582, p<0.05), total organic carbon content (r=0.325, p<0.05), total nitrogen content (r=0.570, p<0.01), and available phosphorus content (r=0.311, p<0.05) were closely related to the soil ARG subtypes; the dissolved organic carbon (DOC) content (r=0.275, p<0.05) and available potassium content (r=0.312, p<0.05) were correlated with the ARG subtypes, and the DOC content (r=0.201, p<0.05) significantly affected the pathogen community structure in the rice stubble. In summary, straw burning can reduce the relative abundance of X. oryzae in the rice stubble, which suggests that it may be a viable option for preventing and controlling rice bacterial leaf blight; moreover, a reduction in the positive correlation ratio of pathogens-ARGs and in the relative abundance of MGEs after straw burning may inhibit the formation of potentially resistant pathogens. However, given the complexity of soil biological compound pollution, the effects of straw burning on pathogens and ARGs in paddy fields need to be further explored by long-term location experiments.

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Mining key genes of alkaloid synthesis pathway in lotus leaves based on metabolomics and transcriptomics
Shuangqin LI,Zhongyi WANG,Wanyue ZHAO,Longqing CHEN,Huizhen HU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 328-340.   DOI: 10.3785/j.issn.1008-9209.2022.04.291
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In order to explore the molecular mechanism of alkaloid biosynthesis in lotus leaves, metabolomics and transcriptomics sequencing analyses were performed on mature lotus leaves of ‘Taikong Lian’ (high alkaloid content), ‘Juwuba’ (medium alkaloid content) and ‘Dazu Honglian’ (low alkaloid content) cultivars with significant differences in alkaloid content. Metabolomics analysis showed that there were 30, 32 and 14 different metabolites in the three groups of ‘Dazu Honglian’ vs ‘Taikong Lian’ (low vs high alkaloid content), ‘Dazu Honglian’ vs ‘Juwuba’ (low vs medium alkaloid content), and ‘Taikong Lian’ vs ‘Juwuba’ (high vs medium alkaloid content), respectively. These differential metabolites were mainly three types of isoquinoline alkaloids, namely benzylisoquinoline, bis-benzylisoquinoline and aporphine alkaloids, specifically including caaverine, 3-glucosyl-6, 7-dihydroxy-N-methyl-benzyltetrahydroisoquinoline, dopamine, L-tyramine, etc. To further explore the key genes of the above isoquinoline alkaloid biosynthesis pathway, the transcriptomics sequencing analysis of three cultivars were performed. The numbers of differentially expressed genes (DEGs) among the three groups (‘Dazu Honglian’ vs ‘Taikong Lian’, ‘Dazu Honglian’ vs ‘Juwuba’, and ‘Taikong Lian’ vs ‘Juwuba’) were 2 866, 2 739 and 3 932, respectively; and there were 379 DEGs in common, which contained isoquinoline alkaloid biosynthesis pathway genes. Combined with the results of metabolomics analysis, six key DEGs, including NnCYP80G, Nn6OMT, NnTYDC, NnNCS, NnRAV and NnERF, were finally screened and verified by real-time fluorescent quantitative polymerase chain reaction, which can be used for subsequent gene function verification and molecular regulation network analysis.

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Advances in the pathogenic classification, epidemiological monitoring and control of soybean anthracnose
Jianfei LI,Xiaoxiao WANG,Yue SHU,Qi HUANG,Guixiang TANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (4): 463-471.   DOI: 10.3785/j.issn.1008-9209.2023.03.162
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Anthracnose is a major disease caused by Colletotrichum Cordathat significantly reduces yield and quality of soybeans. This disease is prevalent in various regions, including the main production areas of fresh soybeans in the middle and lower reaches of the Yangtze River in China, South Asia, Southeast Asia, and South America. Based on the harm, classification and incidence cycle of soybean anthracnose, we reviewed the pathogenic identification, incidence prediction in the field, green prevention and control and other related research progress, aiming to provide a theoretical basis for field surveillance and comprehensive control of soybean anthracnose.

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Study on quality characteristics and key flavor chemical constituents of Zhejiang typical particle-shaped famous green teas
Fangyuan FAN,Qiuwen YU,Yilin TONG,Shuying GONG,Jianqiao QI,Fujiong WEI,Xu ZHANG
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 358-367.   DOI: 10.3785/j.issn.1008-9209.2022.04.292
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This study investigated the sensory traits and flavor chemical constituents and their contents of Zhejiang typical particle-shaped famous green teas including Juenong·Cuiming (from Shaoxing, Zhejiang, China), Pingshuirizhu (Shaoxing, Zhejiang, China), and Fenghuaquhao (Ningbo, Zhejiang, China). The differences in sensory characteristics and their key chemical contributors were analyzed. The results showed that there were obvious differences in appearance traits, including tightness, pekoe and color of different typical samples. The Juenong·Cuiming samples show fine and compact granules. The surface of Fenghuaquhao is covered with pekoe and its color is emerald green. For tea taste, Juenong·Cuiming has sweet and mellow taste, while the taste of Fenghuaquhao and Pingshuirizhu are mellow and thick. The gallic acid, catechin, epigallocatechin, epigallocatechin gallate, glutamic acid, arginine, methionine, theanine and γ-aminobutyric acid, etc. were identified as key differential compounds. For tea aroma, Fenghuaquhao is characterized by fresh aroma, while others have tender aroma and moderate toasty aroma. The volatile constituents with fresh and floral aroma such as dihydrolinalool, 1-octanol, and nerol, and volatiles with fruit and sweet aroma such as (E)-nerolidol, 1-nonanal, decanal, 3-nonen-2-one, and α-calacorene, and volatiles produced under high-temperature processing, such as β-ionone epoxide, dihydroactinidiolide, and tea pyrrole, were analyzed to be related with sensory aroma differences. In conclusion, there are obvious differences in appearance, taste, and aroma traits among particle-shaped green teas from different tea producing areas. The sensory differences of particle-shaped green teas were caused by diverse key chemical constituents and their contents, which were induced by tea cultivars, growing environment, and tea processing technology.

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Function and mechanism of cohesin REC8 during meiosis
Jingling DAI,Chao YU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (5): 607-617.   DOI: 10.3785/j.issn.1008-9209.2022.08.121
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Cohesin is a functionally and evolutionarily conserved multi-subunit protein complex that is required for sister chromatid cohesion and chromatin loop structure in both mitosis and meiosis. The meiotic cell-cycle consisting of one DNA replication and two successive rounds of chromosome segregation completes the segregation of homologous chromosomes and sister chromatids. Cohesin is crucial for faithful and proper segregations. There is a group of distinctive cohesin subunits that are only expressed in meiotic cells. The study of meiosis-specific cohesin is of great significance for understanding chromosome architecture and dynamics in meiosis. REC8 is a typical meiosis-specific cohesin subunit that plays essential roles in sister chromatid cohesion and meiotic chromosome events. Here, we review the function and mechanism of meiotic cohesin REC8 based on the current study and hypothesize that phosphorylation modification and microRNAs (miRNAs) could be the subsequent research directions of REC8.

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Development of whole-genome simple sequence repeat markers in Proto-salanx chinensis and their test in different ecological populations
Xuemei TANG,Yanfeng ZHOU,Di’an FANG,Yuting LUO,Minying ZHANG,Shulun JIANG,Xizhao ZHANG,Fei PENG,Yang YOU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (3): 413-423.   DOI: 10.3785/j.issn.1008-9209.2022.04.191
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Krait software was used to analyze the distribution characteristics of perfect microsatellites in the whole genome of Protosalanx chinensis, which was published in 2020 with a higher degree of splicing, and to develop polymorphic microsatellite DNA (also known as simple sequence repeat) markers. The results showed that a total of 587 554 perfect microsatellite loci were obtained in the whole genome of P. chinensis, with a total sequence length of 11 803 017 bp, accounting for 2.53% of the whole genome length. Among six repeat types of microsatellites, the number of dinucleotide was the largest (401 585, accounting for 68.35%). In the 99 pairs of primers designed for microsatellite loci, 39 were polymorphic. Among them, 14 microsatellite markers with favorable polymorphism were selected to test one representative population selected from each of the migratory, landlocked, and introduced populations. The results indicated that 14 microsatellite markers with favorable polymorphism could achieve effective amplification in the three representative populations. The genetic diversity and genetic structure of the three populations were analyzed, and it was found that the migratory population (Chongming Island population) had abundant genetic variation (the mean expected heterozygosity is 0.614, and the mean polymorphism information content is 0.576), which could be clustered into a genetic group different from the freshwater populations [including Taihu Lake population (landlocked) and Lianhuan Lake population (introduced)], and there were large genetic distance and extremely high level of genetic differentiation level between them [the genetic differentiation index (Fst) is higher than 0.25, P<0.05]. The genetic variations between the two freshwater populations (Taihu Lake and Lianhuan Lake populations) were relatively scarce and the genetic distance between them was small. Although there was significant genetic differentiation between them, the genetic differentiation level was relatively low (Fst=0.102, P<0.05). These results indicate that the migratory population has potential conservation value of germplasm resource, which provide basis for the development of microsatellite markers and construction of genetic maps, and furthermore provide references for the subsequent evaluation of large-scale population germplasm resources of P. chinensis.

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Identification and expression analysis of myo-inositol oxygenase gene family in Brassica napus L.
Shubing CHEN,XU Zishu,Qian HUANG,Hui ZHANG,Kangni ZHANG,Yi DUAN,Yue’e SUN,Weijun ZHOU,Ling XU
Journal of Zhejiang University (Agriculture and Life Sciences)    2023, 49 (4): 484-496.   DOI: 10.3785/j.issn.1008-9209.2022.09.281
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Myo-inositol oxygenase (MIOX) catalyzes the conversion of myo-inositol to glucuronic acid and plays an important role in response to biotic and abiotic stresses. In this study, the genome-wide identification and expression pattern analysis of the MIOX family gene in Brassica napus L.(BnMIOX) were conducted. The results showed that the BnMIOX family included 12 members distributed across nine chromosomes. According to the characteristics of MIOX gene domain, the phylogenetic tree of B. napus, Arabidopsisthaliana, B. rapa and B. oleracea could be divided into subfamilies Ⅰ, Ⅱ and Ⅲ. No tandem repeat gene pairs were found in the collinearity analysis, and all of them were large segment replication genes, demonstrating that segmental duplication and whole-genome duplication were the main driving forces for the MIOX gene family amplification in B. napus. The transcriptomic data indicated that BnMIOX genes showed different temporal and spatial expression patterns in different tissues and different growth and development processes. Expression profiles under different stresses demonstrated that the expression of BnMIOX1 gene was obviously induced under drought and salt stresses, while BnMIOX1, BnMIOX2, and BnMIOX9 genes had significant responses to drought, salt, abscisic acid (ABA), and cold stresses. The results of the protein interaction network analysis further showed that the BnMIOX interacted with proteins including GLCAK, PIS1, VTC2, VTC4, and PDF2.1, implying that BnMIOX genes play key roles in improving the resistance of B. napus. This study provides an important basis for further investigation of the function of BnMIOX genes.

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