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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (5): 644-650    DOI: 10.3785/j.issn.1008-9209.2023.06.161
Special Topic: Major Bacterial and Viral Diseases in Crops     
Research progress on the molecular basis of plant-Ralstonia solanacearum recognition
Zhiliang XIAO1(),Aiguo YANG1,Meixiang ZHANG2()
1.Institute of Tobacco Research, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China
2.College of Life Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi, China
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Abstract  

Ralstonia solanacearum causes bacterial wilt disease in multiple crops, which severely threatens the global crop safety production. This pathogen exhibits high genetic diversity and evolves rapidly, and there is a lack of effective disease-resistant varieties in production, which brings great challenges for effective disease control. Identifying receptor proteins in plants that recognize associated molecular patterns or effectors of R. solanacearum and elucidating their molecular recognition mechanisms can provide clues to understand the mechanisms of plant-pathogen interaction, and lay a basis for the development of broad-spectrum disease resistance in plants. This paper reviewed the recent progress on the molecular basis of plant-R. solanacearum recognition. We mainly focused on the identification and functional analysis of membrane and intracellular receptors that recognize R. solanacearum in plants, as well as the mechanism behind receptor recognition of the associated molecular patterns or effectors from R. solanacearum. Besides, we provide research prospects for the exploration and utilization of disease-resistant resources against R. solanacearum in the future.

Key words： Ralstonia solanacearum      recognition      effector      resistance protein     
Received: 16 June 2023      Published: 03 November 2023
CLC:  S432.42  
Corresponding Authors: Meixiang ZHANG     E-mail: xiaozhiliang@caas.cn;meixiangzhang@snnu.edu.cn
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Zhiliang XIAO
Aiguo YANG
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Zhiliang XIAO,Aiguo YANG,Meixiang ZHANG. Research progress on the molecular basis of plant-Ralstonia solanacearum recognition. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(5): 644-650.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2023.06.161     OR     https://www.zjujournals.com/agr/Y2023/V49/I5/644


植物与青枯劳尔氏菌识别的分子基础研究进展

青枯劳尔氏菌（简称“青枯菌”）可在多种作物上引发细菌性青枯病，严重威胁全球作物的安全生产。青枯菌遗传多样性高、变异快，目前生产上缺乏有效的抗病品种，这给青枯病的防治带来了挑战。挖掘植物中识别青枯菌相关分子模式或效应子的受体蛋白，并解析其识别的分子机制，可为认识植物与青枯菌的互作机制提供线索，同时为植物广谱抗病性的创制奠定理论基础。本文综述了近年来植物与青枯菌识别的分子基础研究进展，重点介绍了植物中识别青枯菌的膜上受体和胞内受体的鉴定、功能解析，以及受体与青枯菌相关分子模式或效应子的识别机制，并对今后青枯病防控中抗病资源的挖掘和利用进行了展望。


关键词： 青枯劳尔氏菌,  识别,  效应子,  抗病蛋白 

病原菌蛋白

Pathogen protein

类型

Type

植物识别受体

Recognition receptor

in plant

描述

Description

文献

Reference

冷激蛋白

Cold shock protein

PAMPCORE茄科植物膜上受体CORE识别青枯菌冷激蛋白中保守肽段csp22；CORE的表达具有年龄依赖性[11]

鞭毛蛋白

Flagellin

PAMPGmFLS2a/b大豆中的GmFLS2a/b识别青枯菌鞭毛蛋白中的保守肽段flg22；在本氏烟中异源表达GmFLS2b/GmBAK1可提高本氏烟对青枯病的抗性[13]
PehCPAMP未鉴定PehC具有双重功能，既可以被植物识别，又可以抑制损伤信号触发的免疫[15]
RipP2无毒蛋白RPS4/RRS1RPS4/RRS1是被鉴定的第一个青枯菌胞内识别受体，除了可以识别青枯菌RipP2，还可以识别丁香假单胞菌AvrRPS4[31]
RipBN无毒蛋白SlPtr1番茄胞内受体Ptr1识别青枯菌RipBN，同时也识别丁香假单胞菌AvrRpt2[33]
RipB无毒蛋白ROQ1本氏烟胞内受体ROQ1蛋白不仅可以识别青枯菌RipB，还可识别丁香假单胞菌HopQ1蛋白和黄单胞菌XopQ蛋白[35-36]
RipE1无毒蛋白NbPtr1本氏烟NbPtr1识别青枯菌核心效应子RipE1[46]
RipAA无毒蛋白未知可在烟草上诱导超敏反应，在青枯菌GMI1000中同时敲除RipAA和RipP1后，在烟草上诱导超敏反应的活力显著降低[43]
RipP1无毒蛋白未知可在烟草上诱导超敏反应，在青枯菌GMI1000中同时敲除RipP1和RipAA后，在烟草上诱导超敏反应的活力显著降低[43]
RipAX2无毒蛋白未知茄子AG91-25的主效抗性位点EBWR9可识别青枯菌RipAX2[47]
Table 1 IdentifiedPAMP, avirulence protein and recognition receptor in R. solanacearum
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