Bioinformatics and expression analyses of heat shock protein 90 gene family in <i>Rhododendron ovatum</i>

doi:10.3785/j.issn.1008-9209.2022.08.012

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (5): 708-718 DOI: 10.3785/j.issn.1008-9209.2022.08.012

Horticultural sciences

Bioinformatics and expression analyses of heat shock protein 90 gene family in Rhododendron ovatum

Fengyan LI(

),Fangqian QU,Fangmeng ZHAO,Qi WANG,Hong ZHOU,Liangsheng ZHANG,Yiping XIA(

),Xiuyun WANG(

)

Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China

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Abstract

To explore the roles of heat shock protein 90 (Hsp90) in plant growth development and response to high temperature stress, this study identified the Hsp90 family through bioinformatics methods in the whole genome of Rhododendron ovatum,which is a highly ornamental and widely adaptive species of Rhododendron. Gene structure, cis-acting elements, evolution, and expression pattern of the Hsp90 family were subsequently analyzed. The results showed that 11 members of the Hsp90 family were identified in R. ovatum, which were located on five chromosomes. Cis-acting element analysis showed that all the 11 members of the Hsp90 family were involved in the processes of plant hormone metabolism and stress responses. The phylogenetic tree of Hsp90 family in Arabidopsis thaliana, Solanum lycopersicum, Camellia sinensis, R. simsii, R. delavayi, R. henanense, and R. ovatum was constructed, within four main clades. Evolutionary analysis showed that the Hsp90 family has undergone purification selection during the differentiation of Rhododendron species. Moreover, the expression patterns of Hsp90 family in different tissues and under high temperature treatments indicated that Hsp90 family plays roles in flower development and the response of plants to high temperature stress. This study provides a foundation for further study of the functions of Hsp90 genes in Rhododendron.

Key words： Rhododendron heat shock protein 90 (Hsp90) family high temperature stress expression pattern

Received: 01 August 2022 Published: 03 November 2023

CLC:

S685.21

Corresponding Authors: Yiping XIA,Xiuyun WANG E-mail: 3180100530@zju.edu.cn;ypxia@zju.edu.cn;xiuyunwang@zju.edu.cn

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	Fengyan LI
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	Fangmeng ZHAO
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	Hong ZHOU
	Liangsheng ZHANG
	Yiping XIA
	Xiuyun WANG

Cite this article:

Fengyan LI,Fangqian QU,Fangmeng ZHAO,Qi WANG,Hong ZHOU,Liangsheng ZHANG,Yiping XIA,Xiuyun WANG. Bioinformatics and expression analyses of heat shock protein 90 gene family in Rhododendron ovatum. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(5): 708-718.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.08.012 OR https://www.zjujournals.com/agr/Y2023/V49/I5/708

马银花热激蛋白90基因家族的生物信息学及表达分析

为探究杜鹃花热激蛋白90（heat shock protein 90, Hsp90）在植物生长发育调节和高温胁迫响应中的作用，本研究以杜鹃花属中观赏价值高、环境适应性强的马银花（Rhododendron ovatum）为材料，利用生物信息学方法对其Hsp90家族开展全基因组水平鉴定以及基因结构、顺式作用元件、进化、表达模式等分析。结果表明马银花Hsp90家族有11个成员，分布在5条染色体上。启动子区顺式作用元件分析表明，Hsp90家族成员均参与植物激素代谢和逆境胁迫响应过程。利用马银花、拟南芥、番茄、茶、杜鹃、河南杜鹃、马缨杜鹃的Hsp90家族构建的系统进化树可被划分为4个主要分支。进化分析显示，Hsp90家族在杜鹃花属分化过程中受到纯化选择作用。Hsp90家族在不同组织器官中和高温处理下的表达模式表明，马银花Hsp90家族参与了花器官的发育和植物对高温胁迫的响应。本研究为杜鹃花Hsp90基因功能的深入解析奠定了基础。

关键词： 杜鹃花属, 热激蛋白90家族, 高温胁迫, 表达模式

Table 1 Physicochemical property analysis of protein characterization and prediction of subcellular localization of Hsp90 family members in R. ovatum

Fig. 1 Phylogenetic tree of Hsp90 family in different species

Fig. 2 Collinearity analysis of Hsp90 genes among A. thaliana, R. simsii, C. sinensis, and R. ovatumBlue lines indicate collinearity of Hsp90 genes among different species and grey lines indicate collinearity of other genes.

Fig. 3 Collinearity analysis of Hsp90 family in R. ovatumRed lines indicate collinearity of Hsp90 genes in R. ovatum and grey lines indicate collinearity of other genes.

Table 2 K_a/K_s analysis between Hsp90 homologous gene pairs in R. ovatum and R. simsii

Fig. 4 Conserved motifs and gene structures of Hsp90 family in R. ovatum

Fig. 5 Cis-acting elements analysis of Hsp90 family in R. ovatumThe presence of several colors in one rectangle indicates that this locus may be involved in multiple plant hormone or stress responses.

Fig. 6 Chromosomal localization ofHsp90gene family in R. ovatum

Fig. 7 Heatmap of Hsp90 gene expression in various tissues and organs of R. ovatumFPKM: Fragments per kilobase of exon model per million mapped reads.

Fig. 8 Heatmap of Hsp90 gene expression under different temperatures in R. ovatum


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