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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2021, Vol. 47 Issue (1): 107-117    DOI: 10.3785/j.issn.1008-9209.2019.12.091
Animal sciences & veterinary medicine     
Effects of high temperature stress on antioxidative and non-specific immunity indices of one-year-old Alosa sapidissima
Xincheng YUAN(),Fei JIANG,Yonghai SHI(),Jiabo XU,Yongshi LIU,Pingping DENG
Shanghai Fisheries Research Institute/Shanghai Fisheries Technical Extension Station, Shanghai 200433, China
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Abstract  

In order to investigate the adaptability of Alosa sapidissima to high temperature stress, they were placed under three water temperature gradients (24 ℃, 28 ℃, 30 ℃) for 96 h, and the changes of antioxidant enzyme as well as non-specific immune enzyme activities in their liver and serum were studied at different time (0, 3, 6, 12, 24, 48, 96 h) under different water temperatures. The results showed that in the liver, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in the 28 ℃ and 30 ℃ groups increased over time, and the malondialdehyde (MDA) content increased and then decreased with time, while there were no significant changes in the 24 ℃ group; the activities of SOD and CAT at 48 and 96 h were significantly higher than those in the 24 ℃ group (P＜0.05), and the GSH-Px activity and MDA content in the 30 ℃ group at 96 h were significantly higher than those in the other two groups (P＜0.05). In the serum, SOD activity and MDA content in the 30 ℃ group showed changes that increased first, then decreased, and then increased with time. The SOD activity and MDA content in the 30 ℃ group at 96 h were significantly higher than those in the 24 ℃ and 28 ℃ groups (P＜0.05). The 24 ℃ and 28 ℃ groups remained basically stable. The CAT and GSH-Px activities in the 28 ℃ group at 48 h showed an increasing trend with time, and were significantly greater than the other two groups (P＜0.05), while the CAT and GSH-Px activities in the 30 ℃ group showed a decreasing trend with time, and at 24-96 h, it was significantly smaller than the other two groups (P＜0.05), and the 24 ℃ group remained basically stable. Under the high temperature stress, the alkaline phosphatase (AKP) and acid phosphatase (ACP) activities of liver in the 28 ℃ and 30 ℃ groups decreased with time (P＜0.05), while it was basically remained stable in the 24 ℃ group; the AKP and ACP activities at 48 and 96 h were significantly smaller than those in the 24 ℃ group (P＜0.05). The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the 30 ℃ group increased with time, and the AST activity at 96 h and the ALT activity at 48 h were significantly higher than those in the 24 ℃ group (P＜0.05); the AST activity in the 28 ℃ group increased with time, and was significantly higher at 96 h than that in the 24 ℃ group (P＜0.05), while the ALT activity increased first and then decreased with time. The above results show that high temperature stress has a significant impact on the antioxidant and non-specific immune enzyme activities of A. sapidissima, causing certain damage to its liver. Therefore, in the process of practical production and industrial aquaculture, it is necessary to avoid high temperature stress response from A. sapidissima, and recommend that the breeding temperature is controlled below 28 ℃.

Key words： Alosa sapidissima      high temperature stress      antioxidant index      non-specific immunity     
Received: 09 December 2019      Published: 09 March 2021
CLC:  S  
Corresponding Authors: Yonghai SHI     E-mail: xcyuan2016@163.com;yonghais@163.com
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Xincheng YUAN
Fei JIANG
Yonghai SHI
Jiabo XU
Yongshi LIU
Pingping DENG
Cite this article:

Xincheng YUAN,Fei JIANG,Yonghai SHI,Jiabo XU,Yongshi LIU,Pingping DENG. Effects of high temperature stress on antioxidative and non-specific immunity indices of one-year-old Alosa sapidissima. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(1): 107-117.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.12.091     OR     http://www.zjujournals.com/agr/Y2021/V47/I1/107


高温胁迫对美洲鲥1＋龄鱼种抗氧化与非特异性免疫相关指标的影响

为探讨美洲鲥（Alosa sapidissima）对高温的适应能力，将其分别置于24 ℃（对照组）、28 ℃和30 ℃的不同水温梯度下胁迫96 h，研究在高温胁迫及不同时间（0、3、6、12、24、48和96 h）下美洲鲥肝和血清中抗氧化酶和非特异性免疫酶活性变化。结果显示：在肝中，28 ℃和30 ℃组过氧化氢酶（catalase, CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-Px）、超氧化物歧化酶（superoxide dismutase, SOD）活性随时间增长均呈上升变化，丙二醛（malondialdehyde, MDA）含量呈先升后降的变化趋势，而24 ℃组均无明显变化；其中SOD和CAT活性均于48和96 h时显著大于24 ℃组（P＜0.05），而30 ℃组MDA含量和GSH-Px活性在96 h时均显著大于其他2组（P＜0.05）。血清中，30 ℃组SOD活性和MDA含量均随时间增长呈先升后降再升的变化，在96 h时均显著大于24 ℃和28 ℃组（P＜0.05），而24 ℃和28 ℃组均较稳定。28 ℃组CAT和GSH-Px活性均随时间增长呈上升趋势，并在48 h时显著大于其他2组（P＜0.05），而30 ℃组均呈下降趋势，并在24～96 h时显著小于其他2组（P＜0.05），24 ℃组均基本稳定。28 ℃和30 ℃组肝中碱性磷酸酶（alkaline phosphatase, AKP）和酸性磷酸酶（acid phosphatase, ACP）活性均随时间增长呈降低的变化趋势，均在48和96 h时显著小于24 ℃组（P＜0.05），而24 ℃组基本稳定。30 ℃组谷草转氨酶（aspartate aminotransferase, AST）和谷丙转氨酶（alanine aminotransferase, ALT）活性随时间增长均呈升高趋势，其中，AST活性在96 h和ALT活性在48 h时均显著大于24 ℃组（P＜0.05）；28 ℃组的AST活性随时间增长逐渐增大，而ALT活性呈先升后降的变化趋势，其中AST活性在96 h时显著大于24 ℃组（P＜0.05）。综上所述，高温胁迫对美洲鲥抗氧化和非特异性免疫相关酶活性均产生了显著影响，并对其肝造成了一定的损伤。因此，在实际养殖过程中，应避免因急性高温对美洲鲥产生的应激反应，建议养殖温度控制在28 ℃以下。


关键词： 美洲鲥,  高温胁迫,  抗氧化指标,  非特异性免疫 
Fig. 1 Effects of high temperature stress on SOD activities in liver and serum of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 2 Effects of high temperature stress on CAT activities in liver and serum of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 3 Effects of high temperature stress on GSH-Px activities in liver and serum of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 4 Effects of high temperature stress on MDA contents in liver and serum of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 5 Effects of high temperature stress on AKP activity in liver of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 6 Effects of high temperature stress on ACP activity in liver of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 7 Effects of high temperature stress on AST activity in liver of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
Fig. 8 Effects of high temperature stress on ALT activity in liver of A. sapidissimaDifferent lowercase letters above the bars indicate significant differences among the treatment groups within the same time at the 0.05 probability level.
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[1] Yongshi LIU,Fei JIANG,Yonghai SHI. Evaluation on nutritional components and nutritive quality in body of young fish Alosa sapidissima cultured in two different patterns[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(2): 243-253.