海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响

doi:10.3785/j.issn.1008-9209.2022.02.142

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (1): 105-116 DOI: 10.3785/j.issn.1008-9209.2022.02.142

动物科学与动物医学

海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响

骆贤亮¹(

),刘旺鑫¹,王晶¹,杜鹃^2,³,冯凤琴¹(

)

^1.浙江大学生物系统工程与食品科学学院, 浙江杭州 310058
^2.杭州康源食品科技有限公司, 浙江杭州 310003
^3.浙江诺衍生物科技有限公司, 浙江湖州 313000

Effects of the combination of sea cucumber peptide and Cistanche deserticola on hormone regulation and testicular anti-oxidative damage in acute-exercising mice

Xianliang LUO¹(

),Wangxin LIU¹,Jing WANG¹,Juan DU^2,³,Fengqin FENG¹(

)

^1.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China
^2.Hangzhou Kangyuan Food Technology Co. , Ltd. , Hangzhou 310003, Zhejiang, China
^3.Zhejiang Nuoyan Biotechnology Co. , Ltd. , Huzhou 313000, Zhejiang, China

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摘要：

为初步探究海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响，将小鼠随机分为空白组、对照组、低剂量组合物组（0.3 mg/g）、中剂量组合物组（0.6 mg/g）和高剂量组合物组（2.0 mg/g），用不同剂量的海参肽与肉苁蓉组合物连续给小鼠灌胃42 d，采用急性力竭游泳造模，并于第45天进行交配实验，记录小鼠性行为学参数，并测定其激素水平及抗氧化相关指标。结果表明：组合物中海参肽与肉苁蓉的最佳质量比为2∶1。急性力竭游泳导致小鼠体内出现能量消耗和代谢产物堆积（P＜0.05），而海参肽与肉苁蓉组合物可不同程度地延长小鼠的急性力竭游泳时间，降低其体内乳酸和血尿素氮的含量。急性力竭游泳会引起小鼠睾酮和雌二醇含量下降，但中、高剂量组合物使两者含量显著提升（P＜0.05）；同时，中剂量组合物显著降低了小鼠促卵泡激素和鸢尾素水平（P＜0.01），并显著提高了其促肾上腺皮质激素含量（P＜0.05）；此外，各剂量组合物明显提高了小鼠精子活力，并且显著降低了小鼠精子畸形率，增强了睾丸组织的总抗氧化能力（P＜0.05）。综上所述，在小鼠急性力竭游泳模型中，海参肽与肉苁蓉组合物具有延缓疲劳症状产生、提高睾酮水平、减少精子和睾丸组织氧化损伤的生理活性。本研究为激素水平调节相关功能食品的开发与应用提供了科学依据。

关键词： 海参肽; 肉苁蓉; 急性力竭游泳; 激素; 氧化损伤

Abstract:

The effects of the combination of sea cucumber peptide and Cistanche deserticola (SCPCD) on the hormone regulation and testicular anti-oxidative damage of acute-exercising mice were preliminarily explored in this study. The mice were randomly divided into five groups, including the blank group, the control group, SCPCD-low dose group (SCPCD-L) (0.3 mg/g), SCPCD-medium dose group (SCPCD-M) (0.6 mg/g), and SCPCD-high dose group (SCPCD-H) (2.0 mg/g), and all the mice received continuously intragastric administration by different doses of SCPCD for 42 d. An acute exhaustive swimming (AES) model was conducted, and a mating test was carried out on the 45th day. The sexual behavior ability parameters, hormone levels, and anti-oxidative related indexes were finally measured. The results showed that the optimal mass ratio of sea cucumber peptide and Cistanche deserticola in combination of them was 2∶1. AES led to the energy expenditure and accumulation of metabolites in mice (P＜0.05), but the acute exhaustive swimming time was prolonged and the contents of lactic acid and blood urea nitrogen were reduced by SCPCD treatments of mice. Besides, AES caused decreases in testosterone and estrogen contents, but they were significantly increased in the SCPCD-M and SCPCD-H (P＜0.05). The levels of follicle-stimulating hormone and irisin were significantly reduced (P＜0.01) in SCPCD-M, while the adrenocorticotropic hormone content was increased (P＜0.05). In addition, the sperm malformation rate decreased in SCPCD treatment, while the sperm motility and total antioxidant capacity of testis were significantly improved (P＜0.05). In summary, the SCPCD presented the physiological activities on delaying fatigue, improving testosterone level, and reducing the oxidative damage of sperm and testicular tissue in AES model. These results provide a scientific basis for the development and application of functional foods related to hormone regulation.

Key words: sea cucumber peptide Cistanche deserticola acute exhaustive swimming hormone oxidative damage

收稿日期: 2022-02-14 出版日期: 2023-02-25

CLC:

TS201.2

基金资助: 浙江大学科技合作项目“水产动物源功能蛋白肽产品研究开发”（K横20182914）

通讯作者: 冯凤琴 E-mail: lxlsky008@163.com;fengfq@zju.edu.cn

作者简介: 骆贤亮（https://orcid.org/0000-0003-4162-1316），E-mail：lxlsky008@163.com

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引用本文:

骆贤亮,刘旺鑫,王晶,杜鹃,冯凤琴. 海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(1): 105-116.

Xianliang LUO,Wangxin LIU,Jing WANG,Juan DU,Fengqin FENG. Effects of the combination of sea cucumber peptide and Cistanche deserticola on hormone regulation and testicular anti-oxidative damage in acute-exercising mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(1): 105-116.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.02.142 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I1/105

图1 海参肽分子量、氨基酸组成以及海参肽与肉苁蓉组合物的抗氧化能力

图2 实验流程图和小鼠体质量及急性力竭游泳时间图A中空白组不做急性力竭游泳训练，但参与交配实验。CK：对照组；SCPCD-L：低剂量海参肽与肉苁蓉组合物组；SCPCD-M：中剂量海参肽与肉苁蓉组合物组；SCPCD-H：高剂量海参肽与肉苁蓉组合物组（下同）。*表示实验组与对照组相比在P＜0.05水平差异有统计学意义。

表1 小鼠脏器系数 (%)

表2 小鼠抗疲劳相关指标

表3 小鼠性行为学参数

图3 小鼠血清激素水平

图4 附睾组织病理学（A）、精子形态（B）及精子畸形率（C）

图5 睾丸组织病理分析

图6 小鼠睾丸抗氧化指标

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