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浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (4): 387-392    DOI: 10.3785/j.issn.1008-9209.2012.04.005
生物科学与技术     
湖羊促性腺激素释放激素受体基因(GnRHR)在消化系统中的表达谱分析
张伟, 张君胜, 张尧, 王利红
江苏畜牧兽医职业技术学院,江苏 泰州 225300
Analysis of gonadotropin releasing hormone receptor gene (GnRHR)  expression map in digestive system of Hu sheep
ZHANG Wei, ZHANG Junsheng, ZHANG Yao, WANG Lihong
Jiangsu Animal Husbandry and Veterinary College, Taizhou, Jiangsu 225300, China
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摘要: 建立湖羊促性腺激素释放激素受体(gonadotropin releasing hormone receptor, GnRHR)基因的实时荧光定量聚合酶链反应(RTPCR)检测方法,并采用该方法对湖羊消化系统中胃肠各组织的GnRHR mRNA表达水平进行检测。结果表明:GnRHR  mRNA在瘤胃、网胃、瓣胃、皱胃、十二指肠、空肠、回肠、盲肠、结肠和直肠部位均有表达,其中瘤胃GnRHR mRNA表达量最高,极显著高于其他各组织(P<0.01);网胃GnRHR  mRNA表达量极显著高于十二指肠(P<001),显著高于瓣胃、盲肠和直肠(P<005);皱胃GnRHR mRNA表达量极显著高于瓣胃、空肠、回肠和盲肠(P<001),显著高于结肠和直肠(P<005);结肠GnRHR mRNA表达量显著高于十二指肠和直肠(P<005)。研究证实了GnRHR  mRNA 在湖羊消化系统中有表达,这为GnRH功能的多样性研究提供了新的依据。
Abstract: To detect the gonadotropin releasing hormone receptor  (GnRHR)  mRNA expression pattern in sheep’s digestive system, the SYBR green realtime fluorescence quantitative polymerase chain reaction  (RTPCR)method was established With this method, the  GnRHR  mRNA expression in stomach and intestinum system of Hu sheep was detected The results showed that GnRHR mRNA expressions were detected in rumen, reticulum, omasum, abomasums, duodenum, jejunum, ileum, caecum, colon and rectum The GnRHR mRNA expression in rumen was significantly higher than those in other tissues (P<001); the expression in reticulum was significantly higher than that in duodenum  (P<001)  and  higher than those in omasum, caecum and rectum (P<005); the expression in abomasums was significantly higher than  those in omasum, jejunum, ileum and caecum (P<001),  and higher than  those in colon, rectum (P<005); the expression in colon was  higher than  those in  duodenum and rectum (P<005) It is demonstrated that there are GnRHR mRNA expressions in the digestive system of Hu sheep It provides new evidences for the research field of GnRH functional diversity
出版日期: 2012-07-25
CLC:  Q 75  
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引用本文:

张伟, 张君胜, 张尧, 王利红. 湖羊促性腺激素释放激素受体基因(GnRHR)在消化系统中的表达谱分析[J]. 浙江大学学报(农业与生命科学版), 2012, 38(4): 387-392.

ZHANG Wei, ZHANG Junsheng, ZHANG Yao, WANG Lihong. Analysis of gonadotropin releasing hormone receptor gene (GnRHR)  expression map in digestive system of Hu sheep. , 2012, 38(4): 387-392.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.04.005        http://www.zjujournals.com/agr/CN/Y2012/V38/I4/387

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