A feeding experiment with different diets was conducted on snakehead (Ophiocephalus argus). Experimental groupⅠ was fed with chilled trash fish, and the other experimental groups were fed with puffed feed, of which group Ⅱ, Ⅲ and Ⅳ were added 13%, 16% and 19% flour with carbohydrate content of 17.48%, 19.55% and 21.63%, respectively. The effects of chilled trash fish and different levels of carbohydrate diets on growth performance, plasma glucose content, key enzymes of glucose metabolism and relevant hormone variations of snakehead were investigated at different time after feeding. The results showed that compared with the experimental groupⅠ, the mass gain rate and specific growth rate of all the other experimental groups decreased significantly (P＜0.05), as well as the feed efficiency and feeding rate (P＜0.05). The plasma glucose level of snakehead fed with puffed feed was significantly higher than that of the snakehead fed with chilled trash fish (P＜0.05), and reached the maximum at 9 h after feeding. With the increase of carbohydrate level in puffed feed, the content of hepatic glycogen had an increase trend, and the hepatopancreas index also significantly increased (P＜0.05); moreover, the activities of hexokinase (HK) and glucokinase (GK) in group Ⅱ, Ⅲ and Ⅳ increased significantly than those in groupⅠ(P＜0.05), but the difference of fructose 1, 6 bisphosphatase activity in the treatment groups was not significant (P＞0.05). After feeding 3, 6 and 12 h, compared with the group Ⅰ, the content of insulin in the snakehead fed with puffed feed increased, and in particular the group Ⅱ and Ⅳ increased significantly (P＜0.05); while after feeding 9 and 24 h, the content of insulin in the snakehead of group Ⅳ significantly decreased than that in the group Ⅰ (P＜0.05). In the early period (3, 6 h) after feeding, as carbohydrate levels increased in puffed feed, the content of plasma glucagon in the snakehead of experimental groups significantly increased (P＜0.05) than that in the group Ⅰ, while the level of plasma glucagon after feeding 9 h significantly lowered (P＜0.05).