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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (03): 365-370    DOI: 10.3785/j.issn.1008-9209.2014.06.302
动物科学     
绍兴鸭生长激素基因多态性与生长性能关联性分析
李丽1,2, 徐琪1, 陈阳1, 黄学涛3, 李柳萌4, 陶仲连5, 陈国宏1*
1.扬州大学动物科学与技术学院,江苏 扬州225009; 2.福建省农业科学院畜牧兽医研究所,福州350013; 3.浙江省农业科学院畜牧兽医研究所,杭州310021; 4.诸暨国家级绍兴鸭保种场,浙江 绍兴311800; 5.缙云县畜牧兽医局,浙江 缙云321400
Correlation between the polymorphism of   growth hormone  gene  with the  growth performance  in Shaoxing ducks
Li Li1,2, Xu Qi1, Chen Yang1, Huang Xuetao3, Li Liumeng4, Tao Zhonglian5, Chen Guohong1*
(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China; 2. Institute of  Animal Husbandry and Veterinary Research, Fujian Academy of Agricultural Sciences, Fuzhou  350013, China; 3.  Institute of  Animal Husbandry and Veterinary Research, Zhejiang Academy of Agricultural Sciences, Hangzhou  310021, China; 4. Shaoxing Duck National Conservation Field of ZhuJi, Shaoxing 311800, Zhejiang, China;
5. Jinyun Bureau of Animal Science and Veterinary Medicine, Jinyun 321400, Zhejiang,   China)
 全文: PDF(2142 KB)   HTML ( ) 
摘要： 以绍兴鸭为材料,选择与生长发育密切相关的候选生长激素(growth hormone,GH)基因,通过直接测序方法扫描其单核苷酸多态性(single nucleotide polymorphism,SNP)位点,并分析其与生长性能的关联性.结果表明:在GH基因的2 514 A>G和2 691 C>T处检测到2个SNP位点,且2个基因座均处于Hardy-Weinberg平衡状态。最小二乘法分析表明,GH基因(2 691 C>T)TT基因型个体的6周龄体质量、8周龄体质量、12周龄龙骨长、53周龄胸宽显著高于CT基因型个体(P<0.05);TT基因型53周龄胸肌质量、腹脂质量、腹脂率显著高于CT和CC基因型个体(P<0.05)。研究为在本品种选育中监测绍兴鸭公鸭的生长发育和充分利用淘汰种公鸭肉用价值提供了一定的参考材料,同时从分子水平上丰富了绍兴鸭遗传信息。
Abstract: As excellent germplasm resources in China, Shaoxing duck is famous for its high egg production all over the world. Laying traits, rather than the growth and development traits, are the main breeding criteria in the duck breeding at present. We usually use egg laying traits as the main breeding goal, but ignore the growth and development traits, especially in drake breeding.  Defective growth and development may lead to the loss of duck breeding value and the low fertilization rate, which will finally reduce the economic benefit of drakes. Thus, drakes’ growth traits must be emphasized in breeding of laying ducks. We must pay more attention to the selection of the drakes’ growth traits. This study analyzed the correlation between the polymorphism of growth hormone (GH) gene with the  growth performance  and slaughter in Shaoxing ducks, which provided some reference material for monitoring the growth and development of Shaoxing ducks breeding.
As the candidate genes, GH gene is closely related to the growth and development. The SNP loci of GH were scanned by direct sequencing method. 
The body size was measured at the age of 12 weeks, and the slaughter indexes were measured at the age of 53 weeks. All ducks were sampled randomly according to the group, and the relevance of Shaoxing duck growth performance and slaughter performance were analyzed.
The genomic DNA was extracted by phenol chloroform method from blood. All data were sampled randomly according to the group.  
According to the published GH gene sequence in GenBank, the primers were designed to amplify the sequences of  intron 2, exon 4 and exon 5. The SNP loci of GH were scanned by direct sequencing method, and the sequences were compared by using DNAStar to look for the polymorphic loci. The genotype and allele frequencies were calculated  and tested by Hardy-Weinberg balance.   The correlation between the genotypes and traits was calculated by  using  statistical software JMP 4.0 (SAS Institute, 2002) to estimate the least square means of traits.   The results showed two loci: GH 2 514 A>G, GH 2 691 C>T. The χ2 test revealed that all loci were in the balance of Hardy-Weinberg (P>0.05 ). For GH (2 514 A>G & 2 691 C>T), 6-week body mass, 8-week body mass, 12-week keel bone length, 53-week chest width of the individuals with genotype TT were higher than the genotype CT. The breast muscle  mass, abdominal fat mass and abdominal fat percentage of individuals with genotype TT were significantly higher than those  with genotype CT and CC (P<0.05). It is  suggested that GH gene may be the major gene which directly control the abdominal fat and breast muscle related traits, or may be tightly linked with the major genes indirectly.
出版日期: 2015-05-20
CLC:  S 834.89  
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李丽
徐琪
陈阳
黄学涛
李柳萌
陶仲连
陈国宏

引用本文:

李丽, 徐琪, 陈阳, 黄学涛, 李柳萌, 陶仲连, 陈国宏. 绍兴鸭生长激素基因多态性与生长性能关联性分析[J]. 浙江大学学报(农业与生命科学版), 2015, 41(03): 365-370.

Li Li, Xu Qi, Chen Yang, Huang Xuetao, Li Liumeng, Tao Zhonglian, Chen Guohong. Correlation between the polymorphism of   growth hormone  gene  with the  growth performance  in Shaoxing ducks. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(03): 365-370.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.06.302    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I03/365

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