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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2022, Vol. 48 Issue (6): 787-796    DOI: 10.3785/j.issn.1008-9209.2022.06.061
Research articles     
Effects of high dose sex pheromone on courtship, mating and oviposition of Chilo suppressalis (Walker) in the wind tunnel
Qianshuang GUO1(),Liling CHEN2,Hua SUI3,Bin YANG3,Fuyan ZHUO4,Xiaoming ZHU4,Rong GUO4,Yongjun DU1()
1.Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2.Jilin Province Agricultural Technology Extension Station, Changchun 130023, China
3.Agricultural Ecological Resource Environment and Rural Energy Management Station of Meihekou City in Jilin Province, Tonghua 135000, Jilin, China
4.National Extension and Service Center of Agricultural Technology, Beijing 100125, China
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Abstract  

To explore the courtship, mating and oviposition behavior of Chilo suppressalis (Walker) in a high dose sex pheromone environment, the mating disruption was simulated in a wind tunnel, and the sex pheromone titers were analyzed in combination with behavioral observation and chemical methods. The results showed that in the wind tunnel, the high dose of Z11-16:Ald and the ternary blend [m(Z11-16:Ald)∶ m(Z9-16:Ald)∶m(Z13-18:Ald)=10∶1∶1.2] of 3.1 mg per spraying significantly inhibited the mating rates and sex pheromone titers of female moths, but no significant differences were found between the two treatments. There were no significant differences in the number of mating pairs of male and female moths and the time interval between each spraying. The mating rates of male moths with the removal of antennae decreased to (8.3±1.1)% and (10.0±8.9)%, respectively, in the environment of spraying or not spraying high dose Z11-16:Ald. The number of laid eggs and egg hatching rates were not affected in the high dose sex pheromone environment. The ages of female moths significantly affected the mating rates with male moths. The mating rates of 1, 3 and 5 day-old female moths with 1 day-old male moths were (60.0±5.2)%, (31.7±6.0)%, and (8.3±3.1)%, respectively. The number of laid eggs and egg hatching rates of female moths decreased with the ages. In conclusion, the high dose sex pheromone Z11-16:Ald and complete blend can inhibit the mating rates and sex pheromone titers of C. suppressalis, and have no impact on the number of laid eggs and egg hatching rates of female moths, but the ages of female moths can affect the number of laid eggs and egg hatching rates; and the decrease in mating rates might be related to the inactivation of olfactory system of male moths.

Key words： Chilo suppressalis (Walker)      sex pheromone      mating disruption      mating      oviposition      age     
Received: 06 June 2022      Published: 27 December 2022
CLC:  S 435.112.1  
Corresponding Authors: Yongjun DU     E-mail: qianshuang@zju.edu.cn;yongjundu@zju.edu.cn
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Qianshuang GUO
Liling CHEN
Hua SUI
Bin YANG
Fuyan ZHUO
Xiaoming ZHU
Rong GUO
Yongjun DU
Cite this article:

Qianshuang GUO,Liling CHEN,Hua SUI,Bin YANG,Fuyan ZHUO,Xiaoming ZHU,Rong GUO,Yongjun DU. Effects of high dose sex pheromone on courtship, mating and oviposition of Chilo suppressalis (Walker) in the wind tunnel. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(6): 787-796.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.06.061     OR     https://www.zjujournals.com/agr/Y2022/V48/I6/787


风洞条件下高剂量性信息素对二化螟求偶、交配和产卵的影响

为了探明在高剂量性信息素环境下二化螟[Chilo suppressalis (Walker)]的求偶、交配和产卵行为，采用风洞试验模拟交配干扰环境，并结合行为观测和化学方法分析性信息素滴度。结果表明：在风洞中，每次释放3.1 mg高剂量Z11-16：Ald或三元混合物[m（Z11-16：Ald）∶m（Z9-16：Ald）∶m（Z13-18：Ald）=10∶1∶1.2]会显著抑制雌蛾的交配率和性信息素滴度，但2个处理间没有显著差异，且雌雄蛾配对数量、喷射间隔时间之间没有显著差异。在喷射或未喷射高剂量Z11-16：Ald环境下，去除触角的雄蛾交配率分别降至（8.3±1.1）%和（10.0±8.9）%。在高剂量性信息素环境下，雌蛾产卵量和卵孵化率没有受到影响；雌蛾日龄显著影响与雄蛾的交配率，1、3、5日龄雌蛾与1日龄雄蛾的交配率分别为（60.0±5.2）%、（31.7±6.0）%、（8.3±3.1）%，且雌蛾随日龄增长产卵量和卵孵化率下降。综上所述，高剂量性信息素环境会抑制二化螟的交配率和性信息素滴度，但不影响雌蛾的产卵量和卵孵化率，而雌蛾日龄对产卵量和卵孵化率有影响；交配率的下降可能与雄蛾嗅觉系统被钝化有关。


关键词： 二化螟,  性信息素,  交配干扰,  交配,  产卵,  日龄 
Fig. 1 Mating rates of female moths in the environment of high dose sex pheromoneA. The released composition is Z11-16:Ald; B. The released composition is m(Z11-16:Ald)∶m(Z9-16:Ald)∶m(Z13-18:Ald)=10∶1∶1.2. CK: Ethanol (the same as below). Different lowercase letters above bars indicate significant differences in mating rates of female moths with the same number of mating pairs at the 0.05 probability level (LSD method).
Fig. 2 Mating number of male moths (A) and mating rates after their antennae were removed (B) in the environment of high dose sex pheromoneSingle asterisk (*) represents significant differences at the 0.05 probability level, and the symbol ns indicates no significant differences at the 0.05 probability level (student’s t-test). The same as Fig. 3 and Fig. 6.
Fig. 3 Effects of high dose sex pheromone treatment on the number of laid eggs (A) and egg hatching rates (B) of female moths
Fig. 4 Effects of high dose sex pheromone treatment on the daily number of eggs laid by female moths with different ages after eclosionThere are no significant differences in the number of eggs laid by female moths of the same age between the treatment group and the control group at the 0.05 probability level (student’s t-test).
Fig. 5 Percentage of female moths releasing sex pheromones in the environment of high dose sex pheromoneSingle asterisk (*) represents significant differences at the 0.05 probability level (chi-square test).
Fig. 6 Titers of sex pheromones released by female moths (A) and the amount of sex pheromones secreted outside the gland (B) in the environment of high dose sex pheromone
Fig. 7 Relationships between ages and mating rates of female mothsDifferent lowercase letters above bars indicate significant differences at the 0.05 probability level (LSD method), and the same as below.
Fig. 8 Effects of the ages of female moths on the number of laid eggs and egg hatching rates
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