黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取工艺优化及活性研究

doi:10.3785/j.issn.1008-9209.2022.08.291

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (6): 813-824 DOI: 10.3785/j.issn.1008-9209.2022.08.291

食品科学

黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取工艺优化及活性研究

张鑫¹(

),朱青永¹,徐慧敏¹,吴梦园¹,陈小娥¹,陈启和²,刘政捷^1,²(

)

^1.浙江海洋大学食品与药学学院，浙江舟山 316022
^2.浙江大学生物系统工程与食品科学学院，浙江杭州 310058

Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens

Xin ZHANG¹(

),Qingyong ZHU¹,Huimin XU¹,Mengyuan WU¹,Xiao’e CHEN¹,Qihe CHEN²,Zhengjie LIU^1,²(

)

^1.College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
^2.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China

全文: PDF(4718 KB) HTML

摘要：

本研究探讨了不同提取工艺对黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取效果的影响。采用超声波辅助提取法，在单因素实验基础上，应用Box-Behnken响应面法优化提取工艺条件，同时考察原伊鲁烷型倍半萜芳基酯的体外抗氧化活性，并采用抑菌圈法评估其对3种常见致病菌的抑菌活性。结果表明：乙酸乙酯为黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯的最佳提取溶剂，在料液比为1∶33（质量体积比）、超声时间为33 min、超声功率为166 W的条件下，原伊鲁烷型倍半萜芳基酯提取量达到10.107 μmol/g。此外，原伊鲁烷型倍半萜芳基酯具有较高的清除2，2-联苯基-1-苦基肼基自由基与羟自由基的能力，并对大肠埃希菌、金黄色葡萄球菌、蜡样芽孢杆菌具有不同程度的抑菌效果。本研究为进一步开发黄绿卷毛菇相关产品提供了数据支持与理论依据。

关键词： 黄绿卷毛菇; 原伊鲁烷型倍半萜芳基酯; 响应面法; 抗氧化; 抑菌

Abstract:

In this study, the effects of different methods on the extraction of protoilludane sesquiterpene aryl esters from Floccularia luteovirens were studied.Using the ultrasonic-assisted extraction method, the extraction process conditions were optimized by Box-Behnken response surface methodology based on a single-factor experiment. The in vitro antioxidant activity of protoilludane sesquiterpene aryl esters was determined, and the antibacterial activities against three common pathogenic bacteria were evaluated via the inhibition zone method. The results showed that ethyl acetate was the most suitable solvent for the extraction of protoilludane sesquiterpene aryl esters. The optimal extraction conditions for accessing protoilludane sesquiterpene aryl esters from F. luteovirens were as follows: solid-liquid ratio of 1∶33 (mass to volume), ultrasonic time of 33 min, and ultrasonic power of 166 W. Under these conditions, the extraction content of protoilludane sesquiterpene aryl esters was 10.107 μmol/g. In addition, the protoilludane sesquiterpene aryl esters had strong scavenging effects against 2, 2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl free radicals, and had different antibacterial effects on Escherichia coli, Staphylococcus aureus, and Bacillus cereus. This study provides data support and a theoretical basis for the further development of F. luteovirens related products.

Key words: Floccularia luteovirens protoilludane sesquiterpene aryl esters response surface methodology antioxidant antibacterial

收稿日期: 2022-08-29 出版日期: 2023-12-25

CLC:

S646

基金资助: 浙江省省属高校、科研院所基本科研业务费项目(2021J007);浙江海洋大学人才引进科研基金项目(11135090721)

通讯作者: 刘政捷 E-mail: zx445452022@163.com;liuzjemail@163.com

作者简介: 张鑫（https://orcid.org/0000-0001-9594-3981），E-mail：zx445452022@163.com

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	张鑫
	朱青永
	徐慧敏
	吴梦园
	陈小娥
	陈启和
	刘政捷

引用本文:

张鑫,朱青永,徐慧敏,吴梦园,陈小娥,陈启和,刘政捷. 黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取工艺优化及活性研究[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(6): 813-824.

Xin ZHANG,Qingyong ZHU,Huimin XU,Mengyuan WU,Xiao’e CHEN,Qihe CHEN,Zhengjie LIU. Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(6): 813-824.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.08.291 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I6/813

表1 响应面实验因素和水平

图1 不同溶剂对原伊鲁烷型倍半萜芳基酯提取量的影响

图2 不同因素对黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取量的影响

表2 Box-Behnken中心组合实验设计与结果

表3 响应面实验的方差分析结果

图3 原伊鲁烷型倍半萜芳基酯提取量与各因素（超声时间、超声功率和料液比）交互作用的响应面与等高线

图4 黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯（样品）对自由基的清除作用

表4 黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯的抑菌活性

30	LI L, PENG C, LI X R, et al. A pair of enantiomeric sesquiterpenoids from florets of Carthamus tinctorius [J]. China Journal of Chinese Materia Medica, 2022, 47(20): 5530-5536. (in Chinese with English abstract) doi: 10.19540/j.cnki.cjcmm.20220612.201
31	师聪,陈学红,解春芝,等.草果不同低共熔溶剂提取物活性成分与其抗氧化活性的相关性分析[J].中国食品添加剂,2022,33(6):24-29. DOI:10.19804/j.issn1006-2513.2022.06.004 SHI C, CHEN X H, XIE C Z, et al. Analysis of the active components in Amomum tsaoko with different low-eutectic solvent and their correlation with antioxidant activity[J]. China Food Additives, 2022, 33(6): 24-29. (in Chinese with English abstract) doi: 10.19804/j.issn1006-2513.2022.06.004
32	李宇,刘翠君,艾伦强,等.恩施产区湖北贝母乙酸乙酯组分挥发性成分与抗菌活性研究[J].浙江农业学报,2021,33(6):1088-1094. LI Y, LIU C J, AI L Q, et al. Study on volatile components and antibacterial activity of ethyl acetate component of Fritillaria hupehensis Hsiao et K. C. Hsia[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1088-1094. (in Chinese with English abstract)
33	DÖRFER M, GRESSLER M, HOFFMEISTER D. Diversity and bioactivity of Armillaria sesquiterpene aryl ester natural products[J]. Mycological Progress, 2019, 18(8): 1027-1037. DOI: 10.1007/s11557-019-01508-z doi: 10.1007/s11557-019-01508-z
34	SIDDIQUI N A, ALAM P, ALREHAILY A J, et al. Optimization of ultrasound-assisted parthenolide extraction from Tarchonanthus camphoratus leaves using response surface methodology: HPTLC and cytotoxicity analysis[J]. Arabian Journal of Chemistry, 2021, 14(6): 103194. DOI: 10.1016/j.arabjc.2021.103194 doi: 10.1016/j.arabjc.2021.103194
35	TRENDAFILOVA A, CHANEV C, TODOROVA M. Ultrasound-assisted extraction of alantolactone and isoalan-tolactone from Inula helenium roots[J]. Pharmacognosy Magazine, 2010, 6(23): 234-237. DOI: 10.4103/0973-1296.66942 doi: 10.4103/0973-1296.66942
36	李英迪,曹艳,夏其乐,等.猴头菇三萜提取工艺优化及其抗氧化活性分析[J].食品工业科技,2021,42(22):153-159. DOI:10.13386/j.issn1002-0306.2020040354 LI Y D, CAO Y, XIA Q L, et al. Optimization of extraction process of Hericium erinaceus triterpenes and its antioxidant activity[J]. Science and Technology of Food Industry, 2021, 42(22): 153-159. (in Chinese with English abstract) doi: 10.13386/j.issn1002-0306.2020040354
37	TIAN Y T, ZENG H L, XU Z B, et al. Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus)[J]. Carbohydrate Polymers, 2012, 88(2): 522-529. DOI: 10.1016/j.carbpol.2011.12.042 doi: 10.1016/j.carbpol.2011.12.042
38	SANTOS K A, GONÇALVES J E, CARDOZO-FILHO L, et al. Pressurized liquid and ultrasound-assisted extraction of α-bisabolol from candeia (Eremanthus erythropappus) wood[J]. Industrial Crops and Products, 2019, 130: 428-435. DOI: 10.1016/j.indcrop.2019.01.013 doi: 10.1016/j.indcrop.2019.01.013
1	戴大日,谢占玲,郭璟,等.黄绿卷毛菇优良菌株筛选及其发酵特性[J].食用菌学报,2020,27(4):115-119. DOI:10.16488/j.cnki.1005-9873.2020.04.014 DAI D R, XIE Z L, GUO J, et al. Strain screening and growth characteristics of Floccularia luteovirens [J]. Acta Edulis Fungi, 2020, 27(4): 115-119. (in Chinese with English abstract) doi: 10.16488/j.cnki.1005-9873.2020.04.014
2	谢占玲,田飞,余静,等.基于LSU和ITS的青藏高原黄绿卷毛菇种群遗传多样性分析[J].菌物学报,2015,34(1):26-37. DOI:10.13346/j.mycosystema.130159 XIE Z L, TIAN F, YU J, et al. The genetic structure analysis of Floccularia luteovirens using LUS and ITS assay[J]. Mycosystema, 2015, 34(1): 26-37. (in Chinese with English abstract) doi: 10.13346/j.mycosystema.130159
39	ILLURI R, KUMAR M, EYINI M, et al. Production, partial purification and characterization of ligninolytic enzymes from selected basidiomycetes mushroom fungi[J]. Saudi Journal of Biological Sciences, 2021, 28(12): 7207-7218. DOI: 10.1016/j.sjbs.2021.08.026 doi: 10.1016/j.sjbs.2021.08.026
40	ABOUHEIF S A, SALLAM S M, SOHAFY S M EL, et al. Optimization of terpene lactones and ginkgolic acids extraction from Ginkgo biloba L. leaves by natural deep eutectic solvents using experimental design and HPTLC-MS analysis[J]. Microchemical Journal, 2022, 176: 107246. DOI: 10.1016/j.microc.2022.107246 doi: 10.1016/j.microc.2022.107246
3	芦笛.唐代藏医籍所载真菌药探析[J].食药用菌,2013,21(4):252-254. LU D. Analysis of fungal drugs in Tibetan medical books in Tang Dynasty[J]. Edible and Medicinal Mushrooms, 2013, 21(4): 252-254. (in Chinese)
41	MA Q, XIE H H, JIANG Y M, et al. Phenolics and sesquiterpenes from litchi pericarp[J]. Journal of Functional Foods, 2014, 9: 156-161. DOI: 10.1016/j.jff.2014.04.020 doi: 10.1016/j.jff.2014.04.020
42	OUAHIOUNE L AIT, WRONA M, BECERRIL R, et al. Ceratonia siliqua L. kibbles, seeds and leaves as a source of volatile bioactive compounds for antioxidant food biopackaging applications[J]. Food Packaging and Shelf Life, 2022, 31: 100764. DOI: 10.1016/j.fpsl.2021.100764 doi: 10.1016/j.fpsl.2021.100764
4	刘昆,蒋俊,郑巧平,等.黄绿卷毛菇研究进展[J].中国食用菌,2019,38(5):1-5. DOI:10.13629/j.cnki.53-1054.2019.05.001 LIU K, JIANG J, ZHENG Q P, et al. Research progress on Floccularia luteovirens [J]. Edible Fungi of China, 2019, 38(5): 1-5. (in Chinese with English abstract) doi: 10.13629/j.cnki.53-1054.2019.05.001
43	DUAN Y C, FENG J, BAI N, et al. Four novel antibacterial sesquiterpene-α-amino acid quaternary ammonium hybrids from the mycelium of mushroom Stereum hirsutum [J]. Fitoterapia, 2018, 128: 213-217. DOI: 10.1016/j.fitote.2018.05.026 doi: 10.1016/j.fitote.2018.05.026
44	MASYITA A, MUSTIKA SARI R, ASTUTI A DWI, et al. Terpenes and terpenoids as main bioactive compounds of essential oils, their roles in human health and potential application as natural food preservatives[J]. Food Chemistry: X, 2022, 13: 100217. DOI: 10.1016/j.fochx.2022.100217 doi: 10.1016/j.fochx.2022.100217
5	刁治民.青海草地黄绿蜜环菌生态学特性及营养价值的研究[J].中国食用菌,1997,16(4):21-22. DIAO Z M. Study on ecology characters and nutrient value of Armillaria luteo-virens in Qinghai grassland[J]. Edible Fungi of China, 1997, 16(4): 21-22. (in Chinese with English abstract)
6	王维恩.超临界CO2萃取黄绿卷毛菇脂肪酸成分的GC-MS分析[J].菌物学报,2015,34(2):321-327. DOI:10.13346/j.mycosystema.140170 doi: 10.13346/j.mycosystema.140170
45	KUDUMELA R G, MAZIMBA O, MASOKO P. Isolation and characterisation of sesquiterpene lactones from Schkuhria pinnata and their antibacterial and anti-inflammatory activities[J]. South African Journal of Botany, 2019, 126: 340-344. DOI: 10.1016/j.sajb.2019.04.002 doi: 10.1016/j.sajb.2019.04.002
6	WANG W E. GC-MS analysis of supercritical carbon dioxide extraction products from Floccularia luteovirens [J]. Mycosystema, 2015, 34(2): 321-327. (in Chinese with English abstract) doi: 10.13346/j.mycosystema.140170
7	王欢,王淑敏,陈长宝,等.黄绿卷毛菇对硝酸甘油诱导偏头痛大鼠的镇痛、抗炎作用[J].菌物学报,2020,39(5):917-922. DOI:10.13346/j.mycosystema.190384 WANG H, WANG S M, CHEN C B, et al. Anti-inflammatory and analgetic effects of the aqueous extract of Floccularia luteovirens on NTG-induced migraine in rats[J]. Mycosystema, 2020, 39(5): 917-922. (in Chinese with English abstract) doi: 10.13346/j.mycosystema.190384
8	FENG K, LIU Q H, NG T B, et al. Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens [J]. Biochemical and Biophysical Research Communications, 2006, 345(4): 1573-1578. DOI: 10.1016/j.bbrc.2006.05.061 doi: 10.1016/j.bbrc.2006.05.061
9	GAN X L, BAO X M, LIU B L, et al. Chemical constituents and molecular mechanism of the yellow phenotype of yellow mushroom (Floccularia luteovirens)[J]. Journal of Fungi, 2022, 8(3): 314. DOI: 10.3390/jof8030314 doi: 10.3390/jof8030314
10	LIU Z J, LU H Y, ZHANG X L, et al. The genomic and transcriptomic analyses of Floccularia luteovirens, a rare edible fungus in the Qinghai-Tibet Plateau, provide insights into the taxonomy placement and fruiting body formation[J]. Journal of Fungi, 2021, 7(11): 887. DOI: 10.3390/jof7110887 doi: 10.3390/jof7110887
11	CADELIS M M, COPP B R, WILES S. A review of fungal protoilludane sesquiterpenoid natural products[J]. Antibiotics, 2020, 9(12): 928. DOI: 10.3390/antibiotics9120928 doi: 10.3390/antibiotics9120928
12	KOBORI H, SEKIYA A, SUZUKI T, et al. Bioactive sesquiterpene aryl esters from the culture broth of Armillaria sp.[J]. Journal of Natural Products, 2015, 78(1): 163-167. DOI: 10.1021/np500322t doi: 10.1021/np500322t
13	ZHANG T W, DU Y H, LIU X F, et al. Study on antidepressant-like effect of protoilludane sesquiterpenoid aromatic esters from Armillaria mellea [J]. Natural Product Research, 2021, 35(6): 1042-1045. DOI: 10.1080/14786419.2019.1614577 doi: 10.1080/14786419.2019.1614577
14	KÖNIG S, ROMP E, KRAUTH V, et al. Melleolides from honey mushroom inhibit 5-lipoxygenase via Cys159[J]. Cell Chemical Biology, 2019, 26(1): 60-70. DOI: 10.1016/j.chembiol.2018.10.010 doi: 10.1016/j.chembiol.2018.10.010
15	MISIEK M, HOFFMEISTER D. Sesquiterpene aryl ester natural products in North American Armillaria species[J]. Mycological Progress, 2012, 11(1): 7-15. DOI: 10.1007/s11557-010-0720-3 doi: 10.1007/s11557-010-0720-3
16	BOHNERT M, NÜTZMANN H W, SCHROECKH V, et al. Cytotoxic and antifungal activities of melleolide antibiotics follow dissimilar structure-activity relationships[J]. Phyto-chemistry, 2014, 105: 101-108. DOI: 10.1016/j.phytochem.2014.05.009 doi: 10.1016/j.phytochem.2014.05.009
17	LI H T, TANG L H, LIU T, et al. Protoilludane-type sesquiterpenoids from Armillaria sp. by co-culture with the endophytic fungus Epicoccum sp. associated with Gastrodia elata [J]. Bioorganic Chemistry, 2020, 95: 103503. DOI: 10.1016/j.bioorg.2019.103503 doi: 10.1016/j.bioorg.2019.103503
18	赵岩,张婷文,王德,等.一种蜜环菌及其制品脑心舒等有效部位含量测定方法:CN109541045B[P].2019-08-27. DOI:10.1109/icde48307.2020.00009 ZHAO Y, ZHANG T W, WANG D, et al. : CN109541045B[P]. 2019-08-27. (in Chinese) doi: 10.1109/icde48307.2020.00009
19	SHAHIDI S A. Effect of solvent type on ultrasound-assisted extraction of antioxidant compounds from Ficaria kochii: optimization by response surface methodology[J]. Food and Chemical Toxicology, 2022, 163: 112981. DOI: 10.1016/j.fct.2022.112981 doi: 10.1016/j.fct.2022.112981
20	孙晓波,韦桂凤,王小明,等.Box-Behnken模型优化油茶饼粕多酚提取工艺及抗氧化活性研究[J].食品研究与开发,2022,43(9):109-117. DOI:10.12161/j.issn.1005-6521.2022.09.015 SUN X B, WEI G F, WANG X M, et al. Optimization of the extraction process and antioxidant activity of polyphenols from Camellia seed cake using the Box-Behnken model[J]. Food Research and Development, 2022, 43(9): 109-117. (in Chinese with English abstract) doi: 10.12161/j.issn.1005-6521.2022.09.015
21	徐树来,徐瑶,尤婷婷,等.超高压辅助提取灵芝三萜的工艺优化及抗氧化活性评价[J].食品工业科技,2022,43(20):274-280. DOI:10.13386/j.issn1002-0306.2022030181 XU S L, XU Y, YOU T T, et al. Optimization of ultra-high pressure assisted extraction of the triterpenoids from the Ganoderma lucidum and evaluation of its antioxidant activity[J]. Science and Technology of Food Industry, 2022, 43(20): 274-280. (in Chinese with English abstract) doi: 10.13386/j.issn1002-0306.2022030181
22	谭才邓,朱美娟,杜淑霞,等.抑菌试验中抑菌圈法的比较研究[J].食品工业,2016,37(11):122-125. TAN C D, ZHU M J, DU S X, et al. Study on the inhibition zone method in antimicrobial test[J]. The Food Industry, 2016, 37(11): 122-125. (in Chinese with English abstract)
23	GALVAN D’ALESSANDRO L, KRIAA K, NIKOV I, et al. Ultrasound assisted extraction of polyphenols from black chokeberry[J]. Separation and Purification Technology, 2012, 93: 42-47. DOI: 10.1016/j.seppur.2012.03.024 doi: 10.1016/j.seppur.2012.03.024
24	胡栋宝,黄淑佩,祝晓慧,等.响应面优化鸡油菌多酚的超声辅助提取工艺及其抗氧化活性[J].食品工业科技,2022,43(18):177-184. DOI:10.13386/j.issn1002-0306.2021110285 HU D B, HUANG S P, ZHU X H, et al. Ultrasound-assisted extraction of polyphenols from the mushroom of Cantharelles cibarius and its antioxidant activity[J]. Science and Technology of Food Industry, 2022, 43(18): 177-184. (in Chinese with English abstract) doi: 10.13386/j.issn1002-0306.2021110285
25	张腊腊,胡浩斌,韩明虎,等.响应面优化离子液体超声辅助提取黄花菜总黄酮工艺研究[J].中国调味品,2022,47(3):192-195. DOI:10.3969/j.issn.1000-9973.2022.03.038 ZHANG L L, HU H B, HAN M H, et al. Study on process optimization of ionic liquid ultrasonic-assisted extraction of total flavonoids from Hemerocallis citrina by response surface method[J]. China Condiment, 2022, 47(3): 192-195. (in Chinese with English abstract) doi: 10.3969/j.issn.1000-9973.2022.03.038
26	缪文玉,李冠文,殷孝莹,等.新鲜枸杞叶中黄酮提取工艺的优化及抗氧化性分析[J].山西农业科学,2022,50(5):762-770. DOI:10.3969/j.issn.1002-2481.2022.05.21 MIAO W Y, LI G W, YIN X Y, et al. Optimization of extraction technology and analysis of antioxidant activity of flavonoids from fresh Lycium barbarum leaves[J]. Journal of Shanxi Agricultural Sciences, 2022, 50(5): 762-770. (in Chinese with English abstract) doi: 10.3969/j.issn.1002-2481.2022.05.21
27	AMIRI S, SHAKERI A, SOHRABI M R, et al. Optimization of ultrasonic assisted extraction of fatty acids from Aesculus hippocastanum fruit by response surface methodology[J]. Food Chemistry, 2019, 271: 762-766. DOI: 10.1016/j.foodchem.2018.07.144 doi: 10.1016/j.foodchem.2018.07.144
28	景炳年,魏磊,王伟,等.响应面优化亚麻荠饼粕多酚提取工艺及其抑菌和抗氧化活性研究[J].中国食品添加剂,2022,33(6):37-44. DOI:10.19804/j.issn1006-2513.2022.06.006 JING B N, WEI L, WANG W, et al. Optimization of extraction conditions for polyphenol from Camelina sativa L. by response surface method and its antibacterial and antioxidant activity[J]. China Food Additives, 2022, 33(6): 37-44. (in Chinese with English abstract) doi: 10.19804/j.issn1006-2513.2022.06.006
29	刘娜.南鹤虱倍半萜成分的提取、纯化和鉴定及其抗氧化活性研究[D].山东,济南:山东中医药大学,2016. LIU N. Study on the extraction, purification, identification and the antioxidant activity of the sesquiterpenoid compound from Carotae fructus [D]. Jinan, Shandong: Shandong University of Traditional Chinese Medicine, 2016. (in Chinese with English abstract)

[1]	罗裳,王志远,李长威,蒋娜,韩永亮,荣湘民,杨兰. 过氧化钙缓解冬油菜苗期渍害胁迫的效应研究[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(4): 516-525.
[2]	金蓉,蔡子涵,赵以勒,沈超怡,杨智超,吴迪,陈昆松. 负载百里酚的聚己内酯纳米纤维膜制备及保鲜应用研究[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(3): 368-375.
[3]	唐政,余越,刘羽飞,岑海燕. 田间作物表型获取无人车平台主体结构设计与优化[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(2): 280-292.
[4]	陈海天,周学军,沙军静,李晓丽,王瑾,何勇. 铅气溶胶胁迫下茶树叶片生理生化指标变化及光谱快速检测[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(1): 117-128.
[5]	李子婧,刘帆,汤胜,马庆旭,韩科峰,吴良欢. *纤维素降解菌长枝木霉菌（Trichoderma longibrachiatum）ZJ-10的筛选及产酶条件优化*[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(5): 614-624.
[6]	陈一,冀飞,刘建新,王迪铭. 日粮添加硒代蛋氨酸锌对泌乳盛期奶牛泌乳性能和血浆生化指标的影响[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(4): 517-524.
[7]	王振杰,张康,梁莉,熊晴晴,杜华华. 铁饱和度对乳铁蛋白抑菌活性的影响[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(3): 377-382.
[8]	周认,蔡宇,林恬逸,柴明良. 模拟干旱胁迫下褪黑素和表油菜素内酯对沟叶结缕草长期继代培养愈伤组织再生的影响[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(1): 36-44.
[9]	朱泰霖,王慧心,陈杰标,王岳,曹锦萍,李鲜,孙崇德. 不同品种柑橘果实的类黄酮分离纯化及其抗氧化活性研究[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(6): 704-718.
[10]	袁新程,蒋飞,施永海,徐嘉波,刘永士,邓平平. 高温胁迫对美洲鲥1^＋龄鱼种抗氧化与非特异性免疫相关指标的影响[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(1): 107-117.
[11]	陈智雄,谭礼强,张钰玉,胥伟,赵许朋,谢文钢,刘阳,刘燕. 藏茶茶褐素组分特征及其清除1，1-二苯基-2-三硝基苯肼自由基活性的比较[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(5): 582-590.
[12]	陈小连,宋文静,周泉勇,宋琼莉,邹志恒,刘林秀,胡利珍,韦启鹏,严景生,达列亚•阿合买提null. 广东紫珠提取物对母猪繁殖性能、免疫、抗氧化功能及肠道微生物的影响[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(3): 360-368.
[13]	陈海念,冯蓉,杨胜竹,曹本福,文明江,刘丽,陆引罡. 1株生防菌的鉴定及其发酵条件优化[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(2): 177-188.
[14]	顾天天,田勇,周玮,刘国发,陈黎,曾涛,吴信生,徐琪,陈国宏,卢立志. 上笼应激对绍兴鸭十二指肠组织结构、抗氧化能力及基因mRNA表达量的影响[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(2): 234-242.
[15]	Elmon CHINDUDZI,苏帮荣,郭伊,钟镇涛,Jane MAKONI,祝水金,陈进红. Heat tolerance evaluation of transgenic cotton germplasms with insect resistance and herbicide tolerance[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(6): 647-656.

Viewed

Full text

Abstract

Cited

Shared

Discussed