广东紫珠提取物对母猪繁殖性能、免疫、抗氧化功能及肠道微生物的影响

doi:10.3785/j.issn.1008-9209.2019.06.210

浙江大学学报（农业与生命科学版）

2020, Vol. 46

Issue (3): 360-368 DOI: 10.3785/j.issn.1008-9209.2019.06.210

动物科学与动物医学

广东紫珠提取物对母猪繁殖性能、免疫、抗氧化功能及肠道微生物的影响

陈小连¹,宋文静¹,周泉勇¹,宋琼莉¹,邹志恒¹,刘林秀¹,胡利珍¹,韦启鹏¹(

),严景生²,达列亚•阿合买提null³

^1.江西省农业科学院畜牧兽医研究所，南昌 330200
^2.江西省井冈山市畜牧兽医局，江西井冈山 343604
^3.新疆维吾尔自治区兽药饲料监察所，乌鲁木齐 830063

Effects of Callicarpa kwangtungensis Chun. extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows

Xiaolian CHEN¹,Wenjing SONG¹,Quanyong ZHOU¹,Qiongli SONG¹,Zhiheng ZOU¹,Linxiu LIU¹,Lizhen HU¹,Qipeng WEI¹(

),Jingsheng YAN², Dalieya?AHEMAITI³

^1.Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
^2.Jinggangshan Animal Husbandry and Veterinary Bureau, Jinggangshan 343604, Jiangxi, China
^3.Procuratorial Supervision of Veterinary Medicine and Feed of Xinjiang Uygur Autonomous Region, Urumqi 830063, China

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摘要：

选择体质量、胎次和预产期相近的妊娠母猪50头，随机分成5组，每组5个重复，每个重复2头，对照组饲喂基础饲粮，试验组分别饲喂在基础饲粮中添加50、100、150和200 mg/kg广东紫珠提取物的试验饲粮，试验周期从母猪妊娠第30天开始至产后第25天结束，研究饲粮中添加广东紫珠提取物对母猪繁殖性能、免疫和抗氧化性能及肠道微生物的影响。结果表明：1）与对照组相比，50和100 mg/kg组仔猪初生体质量均显著高于对照组（P＜0.05），100和150 mg/kg组的仔猪初生窝体质量均显著高于对照组（P＜0.05），150 mg/kg组仔猪断奶体质量及断奶窝体质量均显著高于对照组（P＜0.05）；2）添加广东紫珠提取物可不同程度提高母猪孕中期、分娩时和断奶时血清超氧化物歧化酶活性，降低血清丙二醛含量；3）添加广东紫珠提取物可不同程度降低母猪孕中期、分娩时和断奶时血清白细胞介素1β（interleukin-1β, IL-1β）、白细胞介素6（interleukin-6, IL-6）、肿瘤坏死因子α（tumour necrosis factor-α, TNF-α）等炎症细胞因子水平；4）添加广东紫珠提取物可显著增加母猪肠道乳杆菌属和双歧杆菌属细菌数量，添加100 mg/kg及以上的广东紫珠提取物可降低大肠埃希菌和产气荚膜梭菌数量（P＜0.05）；5）通过建立二次回归曲线，得出母猪饲粮中广东紫珠提取物最适添加量为121～165 mg/kg。综上所述，母猪饲粮中添加121～165 mg/kg广东紫珠提取物可改善母猪的繁殖性能，提高母猪血清抗氧化水平，缓解母猪产后炎症反应，优化母猪肠道菌群结构。

关键词： 广东紫珠提取物; 母猪; 繁殖性能; 免疫功能; 抗氧化性能; 肠道微生物

Abstract:

Fifty sows with similar body mass, parity and due dates were selected and randomly divided into five groups with five replicates per group and two sows per replicate. The sows in the control group were fed on a basal diet, and those in the experimental groups were fed on the basal diet supplemented with 50, 100, 150 and 200 mg/kg Callicarpa kwangtungensis Chun. extract, respectively, from the 30th day of gestation to the 25th day of lactation in order to investigate the effects of C. kwangtungensis extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows. The results showed as follows: 1) Compared with the control group, dietary 50 and 100 mg/kg C. kwangtungensis extract significantly increased the birth body mass, and dietary 100 and 150 mg/kg C. kwangtungensis extract significantly increased the birth litter body mass, and dietary 150 mg/kg C. kwangtungensis extract significantly increased the weaning body mass and weaning litter body mass of piglets (P＜0.05); 2) C. kwangtungensis extract could improve serum superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) content of sows at mid-gestation, farrowing and weaning stages with different degrees; 3) C. kwangtungensis extract could reduce the levels of serum inflammatory cytokine including interleukin-1β (IL-1β), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) of sows at mid-gestation, farrowing and weaning stages with different degrees; 4) C. kwangtungensis extract could significantly increase the beneficial bacterial amounts of Bifidobacterium and Lactobacillus in the sows, and dietary 100 mg/kg and above C. kwangtungensis extract significantly reduced the amount of intestinal Escherichia coli and Clostridium perfringens (P＜0.05); 5）by establishing quadratic regression curves, the optimal addition range of C. kwangtungensis extract in the sows was 121-165 mg/kg. In conclusion, the recommended optimal addition range of C. kwangtungensis extract in the sows is 121-165 mg/kg to improve reproductive performance and serum antioxidant level, alleviate postpartum inflammatory reaction, and optimize intestinal flora structure.

Key words: Callicarpa kwangtungensis Chun. extract sow reproductive performance immune function antioxidant capacity intestinal flora

收稿日期: 2019-06-21 出版日期: 2020-06-03

CLC:

S 828

基金资助: 江西省科技厅科技“三援”项目(20133BFB29008);江西省生猪产业技术体系项目（JXARS-03-营养与饲料岗位）;江西省现代农业科研协同创新专项(JXXTCX2015004);江西省科技专项资金项目(2119044)

通讯作者: 韦启鹏 E-mail: weiqp66@sina.com

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引用本文:

陈小连,宋文静,周泉勇,宋琼莉,邹志恒,刘林秀,胡利珍,韦启鹏,严景生,达列亚•阿合买提null. 广东紫珠提取物对母猪繁殖性能、免疫、抗氧化功能及肠道微生物的影响[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(3): 360-368.

Xiaolian CHEN,Wenjing SONG,Quanyong ZHOU,Qiongli SONG,Zhiheng ZOU,Linxiu LIU,Lizhen HU,Qipeng WEI,Jingsheng YAN, Dalieya?AHEMAITI. Effects of Callicarpa kwangtungensis Chun. extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(3): 360-368.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.06.210 或 http://www.zjujournals.com/agr/CN/Y2020/V46/I3/360

表1 基础饲粮组成及营养水平（风干基础）

表2 广东紫珠提取物对母猪繁殖性能的影响

表3 广东紫珠提取物对母猪血清抗氧化水平的影响

表4 广东紫珠提取物对母猪促炎因子的影响 (pg/mL)

图1 广东紫珠提取物对母猪肠道菌群的影响短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。

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