A 3000 m-class sediment including overlying water pressure-retaining sampler based on heavy-duty remote operated vehicle (ROV) robotic operation and transfer derice matched with the sampler were proposed aiming at the calculation of the leakage flux in the methane leakage area, sample gas composition loss, microbial mortality and organic component decomposition caused by non-pressure-retaining non-gas-tight sampling device. The sampler could achieve low disturbance in the situ packaging of samples. The transfer device realized the separation of overlying water and the transfer of sediment to different incubators under the pressure-retaining condition. The pressure-retaining transfer device transferred the overlying water by compressing the internal volume and transferred the sediment by secondary sampling based on the threshold pressure adjustable relief valve. The results showed that the sampler obtained more than 700 mL pressure-retaining samples in each of the three sea trials in the South China Sea and the pressure drop of sampler was only 1.53 MPa in the 3 000 m sea trial. The sampling rate and the pressure retaining capacity of the sampler were verified in the sea trial. The transfer device completed the transfer of sediment and overlying water separation under 30 MPa high pressure conditions and kept the pressure fluctuation not more than 4.8% during the transfer process. The pressure in the culture kettle decreased only 4.7% relative to the pressure in the sampler after the transfer was completed.
Jin GUO,Jia-wang CHEN,Hao WANG,Ying WANG,Wei WANG,Yu-ping FANG,Peng ZHOU. Design of sampler and associated transfer device of interface between sediment and overlying water. Journal of ZheJiang University (Engineering Science), 2023, 57(5): 1021-1029.
Tab.2Mechanical parameters of stainless steel (17-4PH)
Fig.2Pressure retaining separation and transfer device of sediment and overlying water
Fig.3Section view of push transfer system
Fig.4Section view of sediment culture system
Fig.5Diagram of working principle
Fig.6Model after matching sampler and transfer system
Fig.7Schematic diagram of transferring sediment to culture kettle
Fig.8Sea trial of sediment overlying water interface sampler
Fig.9Seafloor sampling process based on manipulator
Fig.10Samples of sediment, overlying water and dissolved gas obtained by sampler on seafloor
Fig.11Pressure drops of sampler within two hours after sampling in three stations
Fig.12Performance test of sediment overlying water pressure retaining transfer device
Fig.13Pressure fluctuation of transfer device during sample transfer
Fig.14Transfer results of sediment and overlying water samples
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