Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from <i>Floccularia luteovirens</i>

doi:10.3785/j.issn.1008-9209.2022.08.291

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (6): 813-824 DOI: 10.3785/j.issn.1008-9209.2022.08.291

Food sciences

Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens

Xin ZHANG¹(

),Qingyong ZHU¹,Huimin XU¹,Mengyuan WU¹,Xiao’e CHEN¹,Qihe CHEN²,Zhengjie LIU^1,²(

)

^1.College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
^2.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China

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Abstract

In this study, the effects of different methods on the extraction of protoilludane sesquiterpene aryl esters from Floccularia luteovirens were studied.Using the ultrasonic-assisted extraction method, the extraction process conditions were optimized by Box-Behnken response surface methodology based on a single-factor experiment. The in vitro antioxidant activity of protoilludane sesquiterpene aryl esters was determined, and the antibacterial activities against three common pathogenic bacteria were evaluated via the inhibition zone method. The results showed that ethyl acetate was the most suitable solvent for the extraction of protoilludane sesquiterpene aryl esters. The optimal extraction conditions for accessing protoilludane sesquiterpene aryl esters from F. luteovirens were as follows: solid-liquid ratio of 1∶33 (mass to volume), ultrasonic time of 33 min, and ultrasonic power of 166 W. Under these conditions, the extraction content of protoilludane sesquiterpene aryl esters was 10.107 μmol/g. In addition, the protoilludane sesquiterpene aryl esters had strong scavenging effects against 2, 2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl free radicals, and had different antibacterial effects on Escherichia coli, Staphylococcus aureus, and Bacillus cereus. This study provides data support and a theoretical basis for the further development of F. luteovirens related products.

Key words： Floccularia luteovirens protoilludane sesquiterpene aryl esters response surface methodology antioxidant antibacterial

Received: 29 August 2022 Published: 25 December 2023

CLC:

S646

Corresponding Authors: Zhengjie LIU E-mail: zx445452022@163.com;liuzjemail@163.com

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Cite this article:

Xin ZHANG,Qingyong ZHU,Huimin XU,Mengyuan WU,Xiao’e CHEN,Qihe CHEN,Zhengjie LIU. Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(6): 813-824.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.08.291 OR https://www.zjujournals.com/agr/Y2023/V49/I6/813

黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取工艺优化及活性研究

本研究探讨了不同提取工艺对黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取效果的影响。采用超声波辅助提取法，在单因素实验基础上，应用Box-Behnken响应面法优化提取工艺条件，同时考察原伊鲁烷型倍半萜芳基酯的体外抗氧化活性，并采用抑菌圈法评估其对3种常见致病菌的抑菌活性。结果表明：乙酸乙酯为黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯的最佳提取溶剂，在料液比为1∶33（质量体积比）、超声时间为33 min、超声功率为166 W的条件下，原伊鲁烷型倍半萜芳基酯提取量达到10.107 μmol/g。此外，原伊鲁烷型倍半萜芳基酯具有较高的清除2，2-联苯基-1-苦基肼基自由基与羟自由基的能力，并对大肠埃希菌、金黄色葡萄球菌、蜡样芽孢杆菌具有不同程度的抑菌效果。本研究为进一步开发黄绿卷毛菇相关产品提供了数据支持与理论依据。

关键词： 黄绿卷毛菇, 原伊鲁烷型倍半萜芳基酯, 响应面法, 抗氧化, 抑菌

Table 1 Factors and levels of response surface experiments

Fig. 1 Effects of different solvents on extraction contents of protoilludane sesquiterpene aryl esters

Fig. 2 Effects of different factors on the extraction contents of protoilludane sesquiterpene aryl esters from F. luteovirens

Table 2 Box-Behnken center combination experiment design and results

Table 3 Results of variance analysis of response surface experiments

Fig. 3 Response surfaces and contours of interactions between the extraction contents of protoilludane sesquiterpene aryl esters and factors (ultrasonic time, ultrasonic power and solid-liquid ratio)

Fig. 4 Scavenging effectson radicals of protoilludane sesquiterpene aryl esters (samples) from F. luteovirens

Table 4 Antibacterial activity of protoilludane sesquiterpene aryl esters from F. luteovirens


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