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Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (6): 813-824    DOI: 10.3785/j.issn.1008-9209.2022.08.291
Food sciences     
Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens
Xin ZHANG1(),Qingyong ZHU1,Huimin XU1,Mengyuan WU1,Xiao’e CHEN1,Qihe CHEN2,Zhengjie LIU1,2()
1.College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
2.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China
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In this study, the effects of different methods on the extraction of protoilludane sesquiterpene aryl esters from Floccularia luteovirens were studied.Using the ultrasonic-assisted extraction method, the extraction process conditions were optimized by Box-Behnken response surface methodology based on a single-factor experiment. The in vitro antioxidant activity of protoilludane sesquiterpene aryl esters was determined, and the antibacterial activities against three common pathogenic bacteria were evaluated via the inhibition zone method. The results showed that ethyl acetate was the most suitable solvent for the extraction of protoilludane sesquiterpene aryl esters. The optimal extraction conditions for accessing protoilludane sesquiterpene aryl esters from F. luteovirens were as follows: solid-liquid ratio of 1∶33 (mass to volume), ultrasonic time of 33 min, and ultrasonic power of 166 W. Under these conditions, the extraction content of protoilludane sesquiterpene aryl esters was 10.107 μmol/g. In addition, the protoilludane sesquiterpene aryl esters had strong scavenging effects against 2, 2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl free radicals, and had different antibacterial effects on Escherichia coli, Staphylococcus aureus, and Bacillus cereus. This study provides data support and a theoretical basis for the further development of F. luteovirens related products.

Key wordsFloccularia luteovirens      protoilludane sesquiterpene aryl esters      response surface methodology      antioxidant      antibacterial     
Received: 29 August 2022      Published: 25 December 2023
CLC:  S646  
Corresponding Authors: Zhengjie LIU     E-mail:;
Cite this article:

Xin ZHANG,Qingyong ZHU,Huimin XU,Mengyuan WU,Xiao’e CHEN,Qihe CHEN,Zhengjie LIU. Optimization of extraction process and activity of protoilludane sesqui-terpene aryl esters from Floccularia luteovirens. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(6): 813-824.

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本研究探讨了不同提取工艺对黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取效果的影响。采用超声波辅助提取法,在单因素实验基础上,应用Box-Behnken响应面法优化提取工艺条件,同时考察原伊鲁烷型倍半萜芳基酯的体外抗氧化活性,并采用抑菌圈法评估其对3种常见致病菌的抑菌活性。结果表明:乙酸乙酯为黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯的最佳提取溶剂,在料液比为1∶33(质量体积比)、超声时间为33 min、超声功率为166 W的条件下,原伊鲁烷型倍半萜芳基酯提取量达到10.107 μmol/g。此外,原伊鲁烷型倍半萜芳基酯具有较高的清除2,2-联苯基-1-苦基肼基自由基与羟自由基的能力,并对大肠埃希菌、金黄色葡萄球菌、蜡样芽孢杆菌具有不同程度的抑菌效果。本研究为进一步开发黄绿卷毛菇相关产品提供了数据支持与理论依据。

关键词: 黄绿卷毛菇,  原伊鲁烷型倍半萜芳基酯,  响应面法,  抗氧化,  抑菌 



因素 Factor

A 料液比



B 超声时间



C 超声功率



Table 1 Factors and levels of response surface experiments
Fig. 1 Effects of different solvents on extraction contents of protoilludane sesquiterpene aryl esters
Fig. 2 Effects of different factors on the extraction contents of protoilludane sesquiterpene aryl esters from F. luteovirens



A 料液比

Solid-liquid ratio

B 超声时间

Ultrasonic time/min

C 超声功率

Ultrasonic power/W

Y 原伊鲁烷型倍半萜芳基酯提取量

Extraction content of protoilludane

sesquiterpene aryl esters/(μmol/g)

Table 2 Box-Behnken center combination experiment design and results




Sum of squares


Degree of freedom


Mean square







模型 Model6.400 090.711 267.50<0.000 1**
A1.510 011.510 0143.09<0.000 1**
B0.314 010.314 029.800.000 9**
C0.321 610.321 630.520.000 9**
A22.070 012.070 0196.52<0.000 1**
B20.373 310.373 335.430.000 6**
C21.320 011.320 0125.11<0.000 1**
AB0.023 310.023 32.210.181 0
AC0.016 110.016 11.530.255 9
BC0.072 410.072 46.870.034 4*
残差 Residual0.073 870.010 5
失拟项 Lack of fit0.032 130.010 71.030.469 4
纯误差 Pure error0.041 640.010 4
总和 Sum6.470 016
决定系数 Coefficient of determination (R2)0.988 6
校正系数 Coefficient of correction (Radj)0.974 0
Table 3 Results of variance analysis of response surface experiments
Fig. 3 Response surfaces and contours of interactions between the extraction contents of protoilludane sesquiterpene aryl esters and factors (ultrasonic time, ultrasonic power and solid-liquid ratio)
Fig. 4 Scavenging effectson radicals of protoilludane sesquiterpene aryl esters (samples) from F. luteovirens



抑菌圈直径 Diameter of inhibition zone/mm


Experimental group


Negative control


Positive control

大肠埃希菌 Escherichia coli9.4±0.38.9±0.124.7±0.1
金黄色葡萄球菌 Staphylococcus aureus15.7±0.18.3±0.125.4±0.2
蜡样芽孢杆菌 Bacillus cereus16.1±0.18.2±0.125.1±0.1
Table 4 Antibacterial activity of protoilludane sesquiterpene aryl esters from F. luteovirens
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