Comparison on characteristics and 1, 1-diphenyl-2-picrylhydrazyl scavenging activity of theabrownine fractions from Tibetan tea

doi:10.3785/j.issn.1008-9209.2020.03.061

Journal of Zhejiang University (Agriculture and Life Sciences)

2020, Vol. 46

Issue (5): 582-590 DOI: 10.3785/j.issn.1008-9209.2020.03.061

Food sciences

Comparison on characteristics and 1, 1-diphenyl-2-picrylhydrazyl scavenging activity of theabrownine fractions from Tibetan tea

Zhixiong CHEN¹(

),Liqiang TAN²(

),Yuyu ZHANG¹,Wei XU²,Xupeng ZHAO¹,Wengang XIE^1,²,Yang LIU¹,Yan LIU¹

^1.College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China
^2.College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

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Abstract

A membrane separation technology was used to make a grading preparation of theabrownines from Tibetan tea, and four theabrownine fractions (TBFs) with the molecular mass of 5-10, ＞10-50, ＞50-100, and ＞100 kDa were got, which were named as TBFs1, TBFs2, TBFs3 and TBFs4, respectively. The physicochemical properties, microstructure, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity of the four TBFs were comparatively studied. The results showed that the molecular mass of theabrownines was not less than 5 kDa, and the four TBFs with different ranges of molecular mass were all weakly acidic, which had different contents of phenolic hydroxyl, carboxyl group, and the complex compounds including proteins and polysaccharides. Spectroscopic analysis showed that the theabrownines were polyhydroxyphenols and were characterized by an absorption peak near 202 nm. The scanning electron microscope observation showed that the aggregation state of the TBFs was significantly different from each other. Within the range of 10-80 μg/mL, there was a positive linear correlation between the theabrownine concentration and its DPPH radical scavenging ability. In addition, the four TBFs showed significantly different antioxidant activities from each other, and the most active fraction was TBFs4 (＞100 kDa).

Key words： Tibetan tea theabrownines membrane separation physicochemical properties antioxidant activity 1, 1-diphenyl-2-picrylhydrazyl

Received: 06 March 2020 Published: 19 November 2020

CLC:

TS 272

Corresponding Authors: Zhixiong CHEN,Liqiang TAN E-mail: zxchan1987@hotmail.com;Tanliqiang@sicau.edu.cn

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	Zhixiong CHEN
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	Wei XU
	Xupeng ZHAO
	Wengang XIE
	Yang LIU
	Yan LIU

Cite this article:

Zhixiong CHEN,Liqiang TAN,Yuyu ZHANG,Wei XU,Xupeng ZHAO,Wengang XIE,Yang LIU,Yan LIU. Comparison on characteristics and 1, 1-diphenyl-2-picrylhydrazyl scavenging activity of theabrownine fractions from Tibetan tea. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(5): 582-590.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.03.061 OR http://www.zjujournals.com/agr/Y2020/V46/I5/582

藏茶茶褐素组分特征及其清除1，1-二苯基-2-三硝基苯肼自由基活性的比较

借助膜分离技术对藏茶茶褐素进行分级制备，得到4个茶褐素组分（theabrownine fractions, TBFs），其分子质量范围分别为5～10 kDa（TBFs1）、＞10～50 kDa（TBFs2）、＞50～100 kDa（TBFs3）和＞100 kDa（TBFs4），并对这4个组分进行理化特性、微观结构和清除1，1-二苯基-2-三硝基苯肼（1, 1-diphenyl-2-picrylhydrazyl, DPPH）自由基活性的比较研究。结果表明，藏茶茶褐素的分子质量不低于5 kDa，4个不同分子质量范围的茶褐素组分均呈弱酸性，具有不同含量的酚羟基、羧基以及蛋白质和多糖等络合物。光谱学分析显示，藏茶茶褐素属多羟基酚类物质，在202 nm附近有一特征吸收峰；扫描电镜观察发现，不同茶褐素组分的聚集形态差异明显。活性分析表明，藏茶茶褐素在10～80 μg/mL范围内其质量浓度与DPPH自由基的清除率呈线性正相关，且各组分间活性差异显著，其中活性最强的茶褐素组分为TBFs4（＞100 kDa）。

关键词： 藏茶, 茶褐素, 膜分离, 理化特征, 抗氧化活性, 1，1-二苯基-2-三硝基苯肼

Fig. 1 Extraction and separation flow diagram of theabrownines from Tibetan teaTBFs: Theabrownine fractions.

Table 1 Yield of theabrownines with different molecular mass distribution

Fig. 2 Ultraviolet-visible spectrogram of theabrownines with different molecular mass distribution

Fig. 3 Infrared spectrogram of theabrownines with different molecular mass distribution

Table 2 Determination result on pH and compositions of theabrownines with different molecular mass distribution

Fig. 4 Scanning electron microscopy of theabrownines with different molecular mass distribution

Fig. 5 DPPH radical scavenging ability of theabrownine fractions with different concentrations

Table 3 Median inhibition concentration (IC₅₀) of different theabrownine fractions on scavenging DPPH radicals


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