Effects of monochromatic lights on the growth and antioxidant enzyme activity of <i>Sanghuangporus sanghuang</i>

doi:10.3785/j.issn.1008-9209.2018.12.281

Journal of Zhejiang University (Agriculture and Life Sciences)

2019, Vol. 45

Issue (5): 542-549 DOI: 10.3785/j.issn.1008-9209.2018.12.281

Crop cultivation & physiology

Effects of monochromatic lights on the growth and antioxidant enzyme activity of Sanghuangporus sanghuang

Yuzhen WANG¹(

),Yao MA^1,²,Qi CHEN¹,Hongyan MA¹,Jing YANG¹,Chen LIU¹,Junzhi LI³(

),Xiaokui MA¹(

)

1. National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/College of Life Sciences, Shaanxi Normal University, Xi’an 710100, China
2. Buchang Pharmaceuticals, Xi’an 710075, China
3. Microbiology Institute of Shaanxi, Xi’an 710043, China

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Abstract

This study aimed to explore the influence of different monochromatic lights on the growth and antioxidant enzyme activity of the mycelia of fungus Sanghuangporus sanghuang. We used a light emitting diode (LED)-based light source as a monochromatic light source, including the blue light (455-490 nm), green light (515-540 nm), yellow light (580-600 nm) and red light (610-710 nm), and taking the white light (390-710 nm) as a control. The growth status, morphological characteristics, malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD) and catalase (CAT) of this strain were studied under the irradiation of different monochromatic lights. The results indicated that, compared with those under the white light, the colony diameter of S. sanghuang increased significantly and the mycelia were denser under the red light, and the fungal growth was inhibited under the blue light and green light, but there was a minimal impact on the fungal growth under the yellow light. The SOD activity of mycelium increased significantly after 9 d of irradiation under the blue light, green light and yellow light, respectively (P＜0.05), while it decreased significantly after 3 d of irradiation under the red light. The CAT activity of mycelium increased under the four monochromatic lights. On the 11th day, the CAT activity increased significantly (P＜0.05), reaching to (31.82±1.60) U/(g?min) under the yellow light. The highest MDA content was observed under the blue light on the 9th day, reaching to (1.07±0.03) mmol/g, which was around 1.16 times under the white light; however, no significant change in the MDA content was observed under the other monochromatic lights. In summary, different monochromatic lights impose different influences on the growth and antioxidant enzyme activity of S. sanghuang, and the red light is more favorable for the growth than the other tested lights.

Key words： monochromatic light Sanghuangporus sanghuang mycelium growth antioxidant enzyme activity

Received: 28 December 2018 Published: 05 December 2019

CLC:

S 567.3

Corresponding Authors: Junzhi LI,Xiaokui MA E-mail: wqcfyz@163.com;xianjunzhi@163.com;biomarkuis@gmail.com;xianjunzhi@163.com

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	Yuzhen WANG
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	Hongyan MA
	Jing YANG
	Chen LIU
	Junzhi LI
	Xiaokui MA

Cite this article:

Yuzhen WANG,Yao MA,Qi CHEN,Hongyan MA,Jing YANG,Chen LIU,Junzhi LI,Xiaokui MA. Effects of monochromatic lights on the growth and antioxidant enzyme activity of Sanghuangporus sanghuang. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(5): 542-549.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2018.12.281 OR http://www.zjujournals.com/agr/Y2019/V45/I5/542

单色光对桑黄生长及其抗氧化酶活性的影响

为探究不同单色光对桑黄生长及其抗氧化酶活性的影响，以白光（390～710 nm）为对照，采用半导体发光二极管（light emitting diode, LED）灯管为单色光光源，选择蓝光（455～490 nm）、绿光（515～540 nm）、黄光（580～600 nm）和红光（610～710 nm）4种单色光，分别对桑黄进行照射培养，观察桑黄的生长状况及形态特征，分析桑黄菌丝体超氧化物歧化酶（superoxide dismutase, SOD）和过氧化氢酶（catalase, CAT）活性及丙二醛（malondialdehyde, MDA）含量的变化。结果表明：与对照白光照射相比，在单色光红光照射下桑黄菌落直径显著增加，菌丝更加密集；在蓝光和绿光照射下，桑黄生长受到抑制；黄光照射对桑黄的生长影响较小。与白光照射相比，在第9天后，蓝光、绿光和黄光照射下桑黄菌丝体SOD活性显著增加（P＜0.05），而在第3天后，红光照射下菌丝体SOD活性显著降低（P＜0.05）。菌丝体CAT活性在蓝光、绿光、黄光和红光长时间照射下比在白光照射下有所增加，在第11天时，CAT活性显著增加（P＜0.05），尤其在黄光照射下达到(31.82±1.60） U/（g?min)。在蓝光照射第9天时，菌丝体MDA含量最高，达到（1.07±0.03） mmol/g，约为白光照射下1.16倍，而在其他单色光照射下MDA含量变化不显著。综上表明，不同单色光照射对桑黄生长及其抗氧化酶活性的影响不同，且红光照射更有利于桑黄生长。

关键词： 单色光, 桑黄, 菌丝生长, 抗氧化酶活性

Fig. 1 Influence of different monochromatic lights on my-celial morphology

Fig. 2 Influence of different monochromatic lights on mycelial dry mass concentration

Table 1 Influence of different monochromatic lights on mycelium growth

Fig. 3 Influence of different monochromatic lights on SOD activitySingle asterisk (*) indicates significant differences between the treatment and the control at the 0.05 probability level.

Fig. 4 Influence of different monochromatic lights on CAT activitySingle asterisk (*) indicates significant differences between the treatment and the control at the 0.05 probability level.

Fig. 5 Influence of different monochromatic lights on the MDA contentSingle asterisk (*) indicates significant differences between the treatment and the control at the 0.05 probability level.


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