| Animal sciences & veterinary medicine |
|
|
|
|
| Effects of Callicarpa kwangtungensis Chun. extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows |
Xiaolian CHEN1,Wenjing SONG1,Quanyong ZHOU1,Qiongli SONG1,Zhiheng ZOU1,Linxiu LIU1,Lizhen HU1,Qipeng WEI1( ),Jingsheng YAN2, Dalieya?AHEMAITI3 |
1.Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2.Jinggangshan Animal Husbandry and Veterinary Bureau, Jinggangshan 343604, Jiangxi, China
3.Procuratorial Supervision of Veterinary Medicine and Feed of Xinjiang Uygur Autonomous Region, Urumqi 830063, China |
|
|
|
Abstract Fifty sows with similar body mass, parity and due dates were selected and randomly divided into five groups with five replicates per group and two sows per replicate. The sows in the control group were fed on a basal diet, and those in the experimental groups were fed on the basal diet supplemented with 50, 100, 150 and 200 mg/kg Callicarpa kwangtungensis Chun. extract, respectively, from the 30th day of gestation to the 25th day of lactation in order to investigate the effects of C. kwangtungensis extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows. The results showed as follows: 1) Compared with the control group, dietary 50 and 100 mg/kg C. kwangtungensis extract significantly increased the birth body mass, and dietary 100 and 150 mg/kg C. kwangtungensis extract significantly increased the birth litter body mass, and dietary 150 mg/kg C. kwangtungensis extract significantly increased the weaning body mass and weaning litter body mass of piglets (P＜0.05); 2) C. kwangtungensis extract could improve serum superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) content of sows at mid-gestation, farrowing and weaning stages with different degrees; 3) C. kwangtungensis extract could reduce the levels of serum inflammatory cytokine including interleukin-1β (IL-1β), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) of sows at mid-gestation, farrowing and weaning stages with different degrees; 4) C. kwangtungensis extract could significantly increase the beneficial bacterial amounts of Bifidobacterium and Lactobacillus in the sows, and dietary 100 mg/kg and above C. kwangtungensis extract significantly reduced the amount of intestinal Escherichia coli and Clostridium perfringens (P＜0.05); 5）by establishing quadratic regression curves, the optimal addition range of C. kwangtungensis extract in the sows was 121-165 mg/kg. In conclusion, the recommended optimal addition range of C. kwangtungensis extract in the sows is 121-165 mg/kg to improve reproductive performance and serum antioxidant level, alleviate postpartum inflammatory reaction, and optimize intestinal flora structure.
|
|
Received: 21 June 2019
Published: 03 June 2020
|
|
|
|
Corresponding Authors:
Qipeng WEI
E-mail: weiqp66@sina.com
|
|
Cite this article:
Xiaolian CHEN,Wenjing SONG,Quanyong ZHOU,Qiongli SONG,Zhiheng ZOU,Linxiu LIU,Lizhen HU,Qipeng WEI,Jingsheng YAN, Dalieya?AHEMAITI. Effects of Callicarpa kwangtungensis Chun. extract on reproductive performance, immune function, antioxidant capacity and intestinal flora of sows. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(3): 360-368.
URL:
http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.06.210 OR http://www.zjujournals.com/agr/Y2020/V46/I3/360
|
广东紫珠提取物对母猪繁殖性能、免疫、抗氧化功能及肠道微生物的影响
选择体质量、胎次和预产期相近的妊娠母猪50头，随机分成5组，每组5个重复，每个重复2头，对照组饲喂基础饲粮，试验组分别饲喂在基础饲粮中添加50、100、150和200 mg/kg广东紫珠提取物的试验饲粮，试验周期从母猪妊娠第30天开始至产后第25天结束，研究饲粮中添加广东紫珠提取物对母猪繁殖性能、免疫和抗氧化性能及肠道微生物的影响。结果表明：1）与对照组相比，50和100 mg/kg组仔猪初生体质量均显著高于对照组（P＜0.05），100和150 mg/kg组的仔猪初生窝体质量均显著高于对照组（P＜0.05），150 mg/kg组仔猪断奶体质量及断奶窝体质量均显著高于对照组（P＜0.05）；2）添加广东紫珠提取物可不同程度提高母猪孕中期、分娩时和断奶时血清超氧化物歧化酶活性，降低血清丙二醛含量；3）添加广东紫珠提取物可不同程度降低母猪孕中期、分娩时和断奶时血清白细胞介素1β（interleukin-1β, IL-1β）、白细胞介素6（interleukin-6, IL-6）、肿瘤坏死因子α（tumour necrosis factor-α, TNF-α）等炎症细胞因子水平；4）添加广东紫珠提取物可显著增加母猪肠道乳杆菌属和双歧杆菌属细菌数量，添加100 mg/kg及以上的广东紫珠提取物可降低大肠埃希菌和产气荚膜梭菌数量（P＜0.05）；5）通过建立二次回归曲线，得出母猪饲粮中广东紫珠提取物最适添加量为121～165 mg/kg。综上所述，母猪饲粮中添加121～165 mg/kg广东紫珠提取物可改善母猪的繁殖性能，提高母猪血清抗氧化水平，缓解母猪产后炎症反应，优化母猪肠道菌群结构。
关键词：
广东紫珠提取物,
母猪,
繁殖性能,
免疫功能,
抗氧化性能,
肠道微生物
|
|
| [1] |
FANG H L, NIE W, ZHU P L, et al. Genetic diversity in Callicarpa kwangtungensis Chun. based on morphological, biochemical and ISSR markers. Journal of Applied Research on Medicinal and Aromatic Plants, 2018,10:41-48. DOI:10.1016/j.jarmap.2018.06.001
doi: 10.1016/j.jarmap.2018.06.001
|
|
|
| [2] |
李珅.广东紫珠的化学成分研究.天津:南开大学,2016:18.
LI S. Studies on the chemical constituents of Callicarpa kwangtungensis Chun. Tianjin: Nankai University, 2016:18. (in Chinese with English abstract)
|
|
|
| [3] |
聂韡,房海灵,邓绍勇,等.不同产地广东紫珠药材苯乙醇苷类成分含量与抗氧化能力的相关性.江苏农业科学,2016,44(3):273-275. DOI:10.15889/j.issn.1002-1302.2016.03.078
NIE W, FANF H L, DENG S Y, et al. Correlation analysis of phenylethanoid glycoside content and antioxidant activity of Callicarpa kwangtungensis Chun. from different origins. Jiangsu Agricultural Sciences, 2016,44(3):273-275. (in Chinese)
doi: 10.15889/j.issn.1002-1302.2016.03.078
|
|
|
| [4] |
ZHOU G P, YU Y, YUAN M M, et al. Four new triterpenoids from Callicarpa kwangtungensis. Molecules, 2015,20(5):9071-9083. DOI:10.3390/molecules20059071
doi: 10.3390/molecules20059071
|
|
|
| [5] |
YUAN M M, ZHONG R J, CHEN G, et al. Two new triterpenoids from Callicarpa kwangtungensis. Journal of Asian Natural Products Research, 2015,17(2):138-142. DOI:10.1080/10286020.2014.969246
doi: 10.1080/10286020.2014.969246
|
|
|
| [6] |
CAI H, XIE Z Y, LIU G H, et al. Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun. PLoS One, 2014,9(3):e93000. DOI:10.1371/journal.pone.0093000
doi: 10.1371/journal.pone
|
|
|
| [7] |
贾安,杨义芳,孔德云,等.广东紫珠超临界提取物的GC-MS成分分析及体外抗菌活性.中国医药工业杂志,2012,43(3):178-181. DOI:10.3969/j.issn.1001-8255.2012.03.007
JIA A, YANG Y F, KONG D Y, et al. Component analysis of SFE-CO2 extracts of Callicarpa kwangtungensis Chun. by GC-MS and antimicrobial activity. Chinese Journal of Pharmaceuticals, 2012,43(3):178-181. (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-8255.2012.03.007
|
|
|
| [8] |
杨必成,邓薇,王枫,等.广东紫珠对脂多糖诱导巨噬细胞炎症因子分泌的影响.中国妇幼保健,2018,33(3):648-650. DOI:10.7620/zgfybj.j.issn.1001-4411.2018.03.57
YANG B C, DENG W, WANG F, et al. Effect of Callicarpa kwangtungensis Chun on secretion of inflammation factors in macrophages induced by lipopolysaccharide. Maternal and Child Health Care of China, 2018,33(3):648-650. (in Chinese with English abstract)
doi: 10.7620/zgfybj.j.issn.1001-4411.2018.03.57
|
|
|
| [9] |
魏运锑.紫珠在兽医临床上的应用.中兽医医药杂志,2009,28(1):71-72.
WEI Y T. Application of Callicarpa kwangtungensis Chun. in veterinary clinic. Journal of Traditional Chinese Veterinary Medicine, 2009,28(1):71-72. (in Chinese)
|
|
|
| [10] |
孔育玲,赖炳群,王道金,等.紫珠草等中草药防治母猪产后三联症试验.江西畜牧兽医杂志,2014(4):10-11.
KONG Y L, LAI B Q, WANG D J, et al. Study on prevention and treatment of postpartum triad in sows with Callicarpa kwangtungensis Chun. Chinese herbs. Jiangxi Journal of Animal Husbandry & Veterinary Medicine, 2014(4):10-11. (in Chinese)
|
|
|
| [11] |
王胜萍,赖柄群,黎雄.紫珠浸膏干粉防治母猪产科疾病试验效果分析.江西畜牧兽医杂志,2017(2):14-15.
WANG S P, LAI B Q, LI X. Analysis of the effect of Callicarpa kwangtungensis Chun. extract dry powder on the prevention and treatment of obstetric diseases in sows. Jiangxi Journal of Animal Husbandry & Veterinary Medicine, 2017(2):14-15. (in Chinese)
|
|
|
| [12] |
EHSAN K, SHUCONG L, PLAIZIER J C, et al. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Applied and Environmental Microbiology, 2009,75(22):7115-7124. DOI:10.1128/AEM.00739-09
doi: 10.1128/AEM
|
|
|
| [13] |
LAN Y F, XUN S, TAMMINGA S, et al. Real-time PCR detection of lactic acid bacteria in cecal contents of Eimeria tenella-infected broilers fed soybean oligosaccharides and soluble soybean polysaccharides. Poultry Science, 2004,83(10):1696-1702.
|
|
|
| [14] |
XIANG Z T, QI H W, HAN G Q, et al. Real-time TaqMan polymerase chain reaction to quantify the effects of different sources of dietary starch on Bifidobacterium in the intestinal tract of piglets. African Journal of Biotechnology, 2013,10(25):5059-5067. DOI:10.1186/1475-2859-10-44
doi: 10.1186/1475-2859-10-44
|
|
|
| [15] |
WISE M G, SIRAGUSA G R. Quantitative detection of Clostridium perfringens in the broiler fowl gastrointestinal tract by real-time PCR. Applied and Environmental Microbiology, 2005,71(7):3911-3916. DOI:10.1128/AEM.71.7.3911-3916.2005
doi: 10.1128/AEM.71.7.3911-3916.2005
|
|
|
| [16] |
LAYTON A, MCKAY L, WILLIAMS D, et al. Development of Bacteroides 16S rRNA gene taqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water. Applied and Environmental Microbiology, 2006,72(6):4214-4224. DOI:10.1128/AEM.01036-05
doi: 10.1128/AEM.01036-05
|
|
|
| [17] |
VANDERHAEGHE C, DEWULF J, DAEMS A, et al. Influence of postpartum cloprostenol treatment in sows on subsequent reproductive performance under field conditions. Reproduction in Domestic Animals, 2010,43(4):484-489. DOI:10.1111/j.1439-0531.2007.00942.x
doi: 10.1111/j.1439-0531.2007.00942.x
|
|
|
| [18] |
陶新,孙雨晴,门小明,等.复合植物提取物替代饲用抗生素对断奶仔猪生长性能和血清指标的影响.动物营养学报,2018,30(1):163-169. DOI:10.3969/j.issn.1006-267x.2018.01.021
TAO X, SUN Y Q, MEN X M, et al. Effects of composite plant extract replacing feed antibiotics on growth performance and serum indexes of weaned piglets. Chinese Journal of Animal Nutrition, 2018,30(1):163-169. (in Chinese with English abstract)
doi: 10.3969/j.issn.1006-267x.2018.01.021
|
|
|
| [19] |
王浩,印遇龙,邓百川,等.植物提取物的特性及其在母猪生产中的应用.动物营养学报,2017,29(11):3852-3862. DOI:10.3969/j.issn.1006-267x.2017.11.003
WANG H, YIN Y L, DENG B C, et al. Plant extracts: characteristics and application in sow production. Chinese Journal of Animal Nutrition, 2017,29(11):3852-3862. (in Chinese with English abstract)
doi: 10.3969/j.issn.1006-267x.2017.11.003
|
|
|
| [20] |
NUNTAPAITOON M, MUNS R, TUMMARUK P. Newborn traits associated with pre-weaning growth and survival in piglets. Asian-Australasian Journal of Animal Sciences, 2018,31(2):237-244. DOI:10.5713/ajas.16.0962
doi: 10.5713/ajas.16.0962
|
|
|
| [21] |
何余湧,陆伟,胡善辉,等.不同体重对哺乳仔猪消化道发育及断奶后生产性能的影响.动物营养学报,2008,20(6):630-635.
HE Y Y, LU W, HU S H, et al. Effects of different body weight on the development of digestive tract and the postweaning performance of suckling piglets. Chinese Journal of Animal Nutrition, 2008,20(6):630-635. (in Chinese with English abstract)
|
|
|
| [22] |
杜莉,夏双双,汪德中,等.博落回及其提取物对哺乳母猪生产性能的影响.家畜生态学报,2017,38(4):44-47. DOI:10.3969/j.issn.1673-1182.2017.04.008
DU L, XIA S S, WANG D Z, et al. Effects of plume poppy (Macleaya cordata) and its extract on performance of lactating sows. Acta Ecologiae Animalis Domastici, 2017,38(4):44-47. (in Chinese with English abstract)
doi: 10.3969/j.issn.1673-1182.2017.04.008
|
|
|
| [23] |
曾丹,黄忠义,苏登楷,等.党参提取物对母猪繁殖性能和血液指标的影响.饲料研究,2016(13):31-34, 40. DOI:10.13557/j.cnki.issn1002-2813.2016.13.008
ZENG D, HUANG Z Y, SU D K, et al. Effects of Codonopsis pilosula extract on performance and blood index of sows. Feed Research, 2016(13):31-34, 40. (in Chinese with English abstract)
doi: 10.13557/j.cnki.issn1002-2813.2016.13.008
|
|
|
| [24] |
SORDILLO L M. Factors affecting mammary gland immunity and mastitis susceptibility. Livestock Production Science, 2005,98(1/2):89-99. DOI:10.1016/j.livprodsci.2005.10.017
doi: 10.1016/j.livprodsci.2005.10.017
|
|
|
| [25] |
FANG Y Z, YANG S, WU G. Free radicals, antioxidants, and nutrition. Nutrition, 2002,18(10):872-879. DOI:10.1016/S0899-9007(02)00916-4
doi: 10.1016/S0899-9007(02)00916-4
|
|
|
| [26] |
龙沈飞,王文涛,朴香淑.连翘的作用机理及其在猪和鸡生产中的应用.动物营养学报,2019,31(4):1499-1510. DOI:10.3969/j.issn.1006-267x.2019.04.005
LONG S F, WANG W T, PIAO X S. Mechanism and application of Forsythia suspensa in pig and chicken production. Chinese Journal of Animal Nutrition, 2019,31(4):1499-1510. (in Chinese with English abstract)
doi: 10.3969/j.issn.1006-267x.2019.04.005
|
|
|
| [27] |
KAAJA R, LAIVUORI H, PULKKI P, et al. Is there any link between insulin resistance and inflammation in established preeclampsia? Metabolism, 2004,53(11):1433-1435. DOI:10.1016/j.metabol.2004.06.009
doi: 10.1016/j.metabol.2004.06.009
|
|
|
| [28] |
洪燕龙.妇炎康泡腾片的制剂工艺、质量标准和广东紫珠化学成分的研究.北京:北京中医药大学,2004:30-38.
HONG Y L. Preparation and quality control of the effervescent tablet and the chemical constituents of Callicarpa kwangtun-gensis Chun. Beijing: Beijing University of Chinese Medicine, 2004:30-38. (in Chinese with English abstract)
|
|
|
| [29] |
周伯庭,李新中,钟广蓉,等.广东紫珠地上部位主要药效学试验.中国现代医学杂志,2006，16(2):204-206.
ZHOU B T, LI X Z, ZHONG G R, et al. Elementary pharmacodynamical studies of aerial part of Callicarpa kwangtungensis Chun. China Journal of Modern Medicine, 2006，16(2):204-206. (in Chinese with English abstract)
|
|
|
| [30] |
QU H H, ZHANG Y M, WANG Y, et al. Antioxidant and antibacterial activity of two compounds (forsythiaside and forsythin) isolated from Forsythia suspensa. Journal of Pharmacy and Pharmacology, 2008,60:261-266. DOI:10.1211/jpp.60.2.0016
doi: 10.1211/jpp.60.2.0016
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
