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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (3): 368-375    DOI: 10.3785/j.issn.1008-9209.2022.07.011
Food sciences     
Fabrication of thymol-loaded polycaprolactone nanofiber membrane for freshness preservation
Rong JIN1(),Zihan CAI2,Yile ZHAO2,Chaoyi SHEN3,Zhichao YANG3,Di WU2,4(),Kunsong CHEN2
1.Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, Zhejiang, China
2.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
3.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China
4.Zhongyuan Institute, Zhejiang University, Zhengzhou 450000, Henan, China
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Abstract  

The existing traditional active preservation packaging has disadvantages such as low porosity and poor air permeability, so the fabrication of nano-scale packaging materials that can release slowly and retain freshness is a hot research topic in the field of storage and logistics of postharvest fruits and vegetables. In this study, polycaprolactone (PCL) nanofiber membranes loaded by thymol (THY) were prepared by the solution blow spinning technique, and the properties of THY/PCL nanofiber membranes were evaluated by material characterization and bacterial inhibition experiments. The results showed that the THY/PCL nanofiber membranes had lower crystallinity and increased thermal stability, while their water vapor permeable properties, surface hydrophobicity, and mechanical properties were not affected. In addition, the THY/PCL nanofiber membranes showed good antibacterial activity against Escherichia coli and Staphylococcus aureus, demonstrating a good application prospect in the field of postharvest preservation of fruits and vegetables.

Key words： solution blow spinning      nanofiber      thymol      polycaprolactone      fruits and vegetables      antibacterial     
Received: 01 July 2022      Published: 25 June 2023
CLC:  S609.3  
Corresponding Authors: Di WU     E-mail: rong@zju.edu.cn;di_wu@zju.edu.cn
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Rong JIN
Zihan CAI
Yile ZHAO
Chaoyi SHEN
Zhichao YANG
Di WU
Kunsong CHEN
Cite this article:

Rong JIN,Zihan CAI,Yile ZHAO,Chaoyi SHEN,Zhichao YANG,Di WU,Kunsong CHEN. Fabrication of thymol-loaded polycaprolactone nanofiber membrane for freshness preservation. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(3): 368-375.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.07.011     OR     https://www.zjujournals.com/agr/Y2023/V49/I3/368


负载百里酚的聚己内酯纳米纤维膜制备及保鲜应用研究

现有的传统活性保鲜包装存在孔隙率低、透气性差等缺点，因此制备纳米级保鲜控释包装材料是果蔬采后贮藏和物流领域中的研究热点。本研究采用溶液吹塑纺丝技术制备负载百里酚（thymol, THY）的聚己内酯（polycaprolactone, PCL）纳米纤维膜，并通过材料表征和抑菌实验对THY/PCL纳米纤维膜的性能进行评价。结果表明：负载THY后，PCL纳米纤维膜的结晶度降低，热稳定性提高，而水蒸气透过性能、表面疏水性与机械性能未受影响。此外，THY/PCL纳米纤维膜对大肠埃希菌和金黄色葡萄球菌表现出良好的抑菌活性，展现了在果蔬采后保鲜领域良好的应用前景。


关键词： 溶液吹塑纺丝,  纳米纤维,  百里酚,  聚己内酯,  果蔬,  抑菌 
Fig. 1 Scanning electron microscope images of PCL nanofibers
Fig. 2 Fourier transform infrared spectra of THY, THY/PCL, and PCL nanofiber membranes
Fig. 3 X-ray diffraction results of THY (A) and THY/PCL nanofiber membranes (B)
Fig. 4 DSC curve (A) and TGA curves (B-C) of PCL and THY/PCL nanofiber membranes
Fig. 5 Mechanical properties of PCL and THY/PCL nanofiber membranesDifferent or same lowercase letters above bars indicate significant or no significant differences at the 0.05 probability level, respectively, and the same as below.
Fig. 6 Water contact angles of PCL and THY/PCL nanofiber membranes
Fig. 7 Water vapor permeability of PCL and THY/PCL nanofiber membranes
Fig. 8 Antibacterial activity of PCL and THY/PCL nanofiber membranes against S. aureus and E. coli
Fig. 9 Inhibition zone diameters of S. aureus (A) and E. coli (B) under the treatments of PCL and THY/PCL nanofiber membranes
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