海藻酸钠/聚氧化乙烯支架电直写成型及影响因素分析

doi:10.3785/j.issn.1008-973X.2025.07.021

浙江大学学报(工学版)

2025, Vol. 59

Issue (7): 1532-1538 DOI: 10.3785/j.issn.1008-973X.2025.07.021

机械与能源工程

海藻酸钠/聚氧化乙烯支架电直写成型及影响因素分析

孙蕾1(

),王春静1,张文磊1,马志鹏2,程永强1

1. 太原理工大学集成电路学院，山西太原 030024
2. 浙江大学航空航天学院，浙江杭州 310058

SA/PEO composite scaffold electrohydrodynamic direct writing fabrication and influencing factors analysis

Lei SUN1(

),Chunjing WANG1,Wenlei ZHANG1,Zhipeng MA2,Yongqiang CHENG1

1. College of Integrated Circuits, Taiyuan University of Technology, Taiyuan 030024, China
2. School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310058, China

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摘要：

为了提升电流体动力直写（EHDDW）在组织工程支架制备中的打印精度，降低设备及生产成本，自主搭建低成本电直写打印平台. 开发不同浓度梯度的海藻酸钠（SA）/聚氧化乙烯（PEO）复合生物墨水，生物学表征及打印测试结果表明，2SA/7PEO复合生物墨水具有良好的细胞相容性及电直写性能. 结合理论分析及实验探究打印参数对复合生物墨水沉积纤维宽度的影响机制，结果表明，增大电压、减小供液流率及提升基板移速均可提高纤维分辨率. 当电压为2 400 V，注射泵流率为200 nL/min，基板移速为300 mm/min时，SA/PEO复合生物墨水的最高打印精度为2.48±0.27 μm. 采用微射流模式/锥射流模式制备SA/PEO复合生物墨水单层/多层网格微图案，证明了自主搭建的电直写打印平台具备制备复杂、多层次生物微结构的潜力.

关键词： 电流体动力直写(EHDDW); 海藻酸钠(SA); 聚氧化乙烯(PEO); 锥射流模式; 组织工程

Abstract:

To improve the printing accuracy of electrohydrodynamic direct writing (EHDDW) in tissue engineering scaffold preparation, and to reduce equipment production costs, a low-cost EHDDW printing platform was constructed independently. Sodium alginate (SA)/polyethylene oxide (PEO) composite bioinks with varying concentration gradients were developed, and the biological characterization and printing test results reveal that the 2SA/7PEO composite bioink exhibits good cytocompatibility and EHDDW printing performance. Through theoretical analysis and experimental study, the influence mechanism of printing parameters on the fiber width of composite bioink was explored. Results show that increasing the voltage, decreasing the fluid flow rate, and accelerating the substrate movement speed can enhance the resolution of fibers. The SA/PEO composite bioink achieved a maximum printing resolution of 2.48±0.27 μm with an applied voltage of 2 400 V, a syringe pump flow rate of 200 nL/min, and a substrate movement speed of 300 mm/min. Single-layer/multi-layer grid micropatterns were prepared using fine-jet mode/cone-jet mode, proving that the self-constructed EHDDW printing platform has the potential to prepare complex and multilayered bio-microstructures.

Key words: electrohydrodynamic direct writing (EHDDW) sodium alginate (SA) polyethylene oxide (PEO) cone-jet mode tissue engineering

收稿日期: 2024-06-14 出版日期: 2025-07-25

CLC:

TH 16

基金资助: 山西省自然科学基金资助项目（202103021223069）.

作者简介: 孙蕾（1990—），女，讲师，从事微纳加工技术与微纳生物传感器研究. orcid.org/0000-0001-8742-0742. E-mail：sunlei@tyut.edu.cn

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引用本文:

孙蕾,王春静,张文磊,马志鹏,程永强. 海藻酸钠/聚氧化乙烯支架电直写成型及影响因素分析[J]. 浙江大学学报(工学版), 2025, 59(7): 1532-1538.

Lei SUN,Chunjing WANG,Wenlei ZHANG,Zhipeng MA,Yongqiang CHENG. SA/PEO composite scaffold electrohydrodynamic direct writing fabrication and influencing factors analysis. Journal of ZheJiang University (Engineering Science), 2025, 59(7): 1532-1538.

链接本文:

https://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2025.07.021 或 https://www.zjujournals.com/eng/CN/Y2025/V59/I7/1532

图 1 电流体动力直写打印平台

图 2 海藻酸钠/聚氧化乙烯水凝胶的制备过程

图 3 海藻酸钠/聚氧化乙烯水凝胶生物学表征

图 4 海藻酸钠/聚氧化乙烯复合生物墨水电直写打印

表 1 复合生物墨水在2种喷射模式下的开启电压

图 5 2SA/7PEO复合生物墨水的不同模式

图 6 施加电压对沉积纤维线宽的影响

图 7 注射泵流率对沉积纤维线宽的影响

图 8 基板移速对沉积纤维线宽的影响

图 9 不同喷射模式的网格结构

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