Breeding and utilization of recessive genic male sterile line LY31AB in <i>Brassica napus</i> L.

doi:10.3785/j.issn.1008-9209.2023.03.291

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (4): 507-515 DOI: 10.3785/j.issn.1008-9209.2023.03.291

Research articles

Breeding and utilization of recessive genic male sterile line LY31AB in Brassica napus L.

Mingchao XU¹(

),Qian HUANG²,Kangni ZHANG²,Xudong ZOU¹,Xingfan CHEN¹,Hong LU¹,Yanhui WANG¹(

),Zhi ZHANG¹(

)

^1.Leshan Academy of Agricultural Sciences, Leshan 614000, Sichuan, China
^2.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China

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Abstract

In order to make full use of the advantages of genic male sterility (GMS), the commercial rapeseed ‘Q1012’ of Brassica napus L. was used as the source to select the double-low and high-quality recessive GMS line LY31AB of B. napus by the pedigree breeding method. LY31AB fertility, resistance, combining ability and other characteristics were analyzed and its heterosis was utilized. The results indicated that the sterility of LY31AB was genetically stable, which was controlled by two pairs of recessive overlapping genes, and showed good resistance to sclerotiniadisease and lodging resistance in the field. LY31AB had high combining ability, wide recovery sources and strong heterosis, and most of its combinations showed obvious heterosis. Four new double-low and high-quality rape varieties, Leyou 8, Deleyou 1632, Wanjiayou 520 and Leyou 7, which were bred using LY31AB and registered, also showed good resistance tosclerotiniadisease and lodging resistance, and were widely in promotion and application. To sum up, LY31AB is a sterile line with clear pedigree, stable genetic characters, good quality, good fertility, good resistance, high combining ability, wide recovery sources and good heterosis utilization value, and has broad application prospects.

Key words： genic male sterile line breeding fertility resistance combining ability heterosis utilization

Received: 29 March 2023 Published: 25 August 2023

CLC:

S565.4

Corresponding Authors: Yanhui WANG,Zhi ZHANG E-mail: 1048515670@qq.com;103434183@qq.com;85943012@qq.com

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	Mingchao XU
	Qian HUANG
	Kangni ZHANG
	Xudong ZOU
	Xingfan CHEN
	Hong LU
	Yanhui WANG
	Zhi ZHANG

Cite this article:

Mingchao XU,Qian HUANG,Kangni ZHANG,Xudong ZOU,Xingfan CHEN,Hong LU,Yanhui WANG,Zhi ZHANG. Breeding and utilization of recessive genic male sterile line LY31AB in Brassica napus L.. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(4): 507-515.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2023.03.291 OR https://www.zjujournals.com/agr/Y2023/V49/I4/507

甘蓝型油菜隐性细胞核雄性不育系LY31AB的选育及利用

为充分利用细胞核雄性不育的优势，以甘蓝型油菜‘Q1012’商品油菜籽为来源，用系谱选育法选育出双低优质甘蓝型油菜隐性细胞核雄性不育系LY31AB，分析其育性、抗性、配合力等特点，并进行杂种优势利用。结果表明：LY31AB不育性遗传稳定，受2对隐性重叠基因控制，且在田间表现出良好的菌核病抗性和抗倒性；LY31AB配合力高、恢复源广、杂种优势强，其所配组合大多表现出明显的杂种优势；利用LY31AB育成并登记的乐油8号、得乐油1632、万家油520、乐油7号4个双低优质油菜新品种也均表现出菌核病抗性和良好的抗倒性，已被大面积推广应用。综上所述，LY31AB是一个选育系谱清楚、遗传性状稳定、品质优、丰产性好、抗性好、配合力高、恢复源广且具有良好杂种优势利用价值的不育系，有着广阔的应用前景。

关键词： 细胞核雄性不育系, 选育, 育性, 抗性, 配合力, 杂种优势利用

Table 1 Design of 20 combinations of combining abilities based on incomplete diallel cross (NC Ⅱ)

Fig. 1 Morphology of LY31AB flower organsA. Alabastrum of sterile plant; B. Alabastrum of fertile plant; C. Flower of sterile plant; D. Flower of fertile plant.

Fig. 2 Morphology of LY31AB mature plants at a cultivation density of 6 000 plants/667 m²A. Sterile plant; B. Fertile plant.

Table 2 Performance of main agronomic traits in LY31AB

Table 3 Fertility performance of LY31AB at flowering stage from 2013 to 2015

Table 4 Fertility status of hybrid offsprings between LY31AB and four inbred lines

Table 5 Resistance performance of LY31AB in the field

Table 6 Performance of the varieties bred from LY31AB in the Sichuan provincial joint experiment of new B. napus varieties

Table 7 Quality testing and resistance identification results of the varieties bred from LY31AB


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