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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (4): 516-525    DOI: 10.3785/j.issn.1008-9209.2023.02.211
Research articles     
Effect of CaO2 on alleviating waterlogging stress of winter rape at seedling stage
Shang LUO1,2(),Zhiyuan WANG1,2(),Changwei LI1,2,Na JIANG1,2,Yongliang HAN1,2,Xiangmin RONG1,2,Lan YANG1,2()
1.College of Resources, Hunan Agricultural University, Changsha 410128, Hunan, China
2.National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, Hunan, China
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Abstract  

To evaluate the effect of CaO2 on alleviating waterlogging stress of winter rape at seedling stage, a pot experiment was conducted with Fengyou 958 (FY958) as experimental material. There were six application amounts of CaO2 (0, 0.7, 1.2, 1.7, 2.2, and 2.7 g/kg) treatments in this study, and the effects of different application amounts of CaO2 on the survival rates, activities of antioxidant enzymes in leaves and fermentative enzymes in roots, and root activity of rape seedlings under waterlogging condition were studied. The results showed that waterlogging stress seriously affected the growth of rape, resulting in a significant decrease in the survival rate, fresh mass and leaf SPAD value of rape seedlings. The application of CaO2 can significantly alleviate the effect of waterlogging stress on rape. Compared with plants without CaO2 treatment under waterlogging condition, the fresh mass and leaf SPAD values of rape seedlings treated with CaO2 significantly increased by 85.4%-108.0% and 24.3%-43.0%, respectively. The activities of antioxidant enzymes in leaves and fermentative enzymes in roots were both significantly reduced. The root activity was significantly increased by 66.7%-316.7%. Moreover, with the increase of the application amounts of CaO2, the effect of alleviating waterlogging stress became more obvious. In summary, waterlogging stress inhibited the normal growth of rape seedlings. The appropriate application amounts of CaO2 (1.7-2.7 g/kg) could reduce the anaerobic respiration of roots, alleviate the effect of peroxidation stress on rape, and increase the root activity and chlorophyll contents (SPAD values) of leaves, so as to improve carbohydrate synthesis in leaves, and thus restore rape seedling growth and ensure later rape yields. This study verified the feasibility of CaO2 in alleviating waterlogging stress of winter rape at seedling stage.

Key words： waterlogging stress      winter rape      CaO2      antioxidant enzyme      anaerobic respiration     
Received: 21 February 2023      Published: 29 August 2023
CLC:  S565.4  
Corresponding Authors: Lan YANG     E-mail: 1219740859@qq.com;644053373@qq.com;helloyanglan@163.com
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Shang LUO
Zhiyuan WANG
Changwei LI
Na JIANG
Yongliang HAN
Xiangmin RONG
Lan YANG
Cite this article:

Shang LUO,Zhiyuan WANG,Changwei LI,Na JIANG,Yongliang HAN,Xiangmin RONG,Lan YANG. Effect of CaO2 on alleviating waterlogging stress of winter rape at seedling stage. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(4): 516-525.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2023.02.211     OR     https://www.zjujournals.com/agr/Y2023/V49/I4/516


过氧化钙缓解冬油菜苗期渍害胁迫的效应研究

为评价过氧化钙缓解冬油菜苗期渍害（渍水）胁迫的效应，以常规冬油菜品种沣油958（FY958）为试验材料，通过盆栽试验，设置不同施用量的过氧化钙（0、0.7、1.2、1.7、2.2、2.7 g/kg）处理，研究渍水条件下，不同的过氧化钙施用量对油菜幼苗存活率、叶片抗氧化酶和根系发酵酶活性以及根系活力等生理指标的影响。结果表明，渍水胁迫严重影响了油菜生长，造成油菜幼苗的存活率、鲜质量和叶片SPAD值显著降低。施用过氧化钙可明显缓解渍水胁迫对油菜的影响。与渍水条件下未施用过氧化钙的植株相比，施用过氧化钙后，油菜幼苗的鲜质量和叶片SPAD值显著提高，幅度分别为85.4%～108.0%和24.3%～43.0%；叶片抗氧化酶与根系发酵酶活性显著降低；同时，根系活力显著提高，增幅为66.7%～316.7%。并且，随着过氧化钙施用量的增加，其改善渍水胁迫的效果更加明显。上述研究结果表明，渍水胁迫会抑制油菜幼苗的生长。适宜的过氧化钙施用量（1.7～2.7 g/kg）能降低根系无氧呼吸，减轻过氧化胁迫对油菜的影响，提高油菜根系活力和叶片叶绿素含量（SPAD值），从而提高叶片碳水化合物合成能力，进而恢复油菜幼苗生长、保证油菜后期产量。本研究验证了过氧化钙缓解冬油菜苗期渍害胁迫的可行性。


关键词： 渍害胁迫,  冬油菜,  过氧化钙,  抗氧化酶,  无氧呼吸 

油菜种子出苗率试验

Emergence rate experiment of rape seeds

油菜渍水试验

Waterlogging experiment of rape

处理

Treatment

播种量/（粒/盆）

Sowing quantity/

(grain/pot)

是否渍水

Waterlogging

or not

CaO2施用量

Application amount

of CaO2/(g/kg)

处理

Treatment

留种数/（株/盆）

Number of reserved

seeds/(plant/pot)

是否渍水

Waterlogging

or not

CaO2施用量

Application amount

of CaO2/(g/kg)

CK0100否0CK20否0
C1100否0.7CK+H2O20是0
C2100否1.2C1+H2O20是0.7
C3100否1.7C2+H2O20是1.2
C4100否2.2C3+H2O20是1.7
C5100否2.7C4+H2O20是2.2
C5+H2O20是2.7
Table 1 Experimental design

处理

Treatment

CaO2施用量

Application amountof CaO2/(g/kg)

出苗率

Emergence rate/%

CK0074.8±3.2a
C10.775.3±3.6a
C21.280.6±4.6a
C31.774.5±3.5a
C42.270.1±2.6a
C52.775.3±7.2a
Table 2 Effects of different application amounts of CaO2 on emergence rates of rape seeds
Fig. 1 Growth phenotypes of rape seedlings under different application amounts of CaO2 and waterlogging conditions
处理

20 d存活率

Survival rate at 20 d

25 d存活率

Survival rate at 25 d

30 d存活率

Survival rate at 30 d

CK96.25±2.39a96.25±2.39a96.25±2.39a
CK+H2O77.50±8.78b66.82±9.22b65.80±7.88b
C1+H2O91.25±5.54a87.65±3.95a87.65±3.95a
C2+H2O81.61±3.74ab80.04±1.92a80.04±1.92a
C3+H2O92.50±3.23a88.97±2.41a86.40±2.54a
C4+H2O90.00±5.40ab87.50±2.78a87.50±2.78a
C5+H2O96.67±2.04a96.67±2.04a89.42±7.50a
Table 3 Effects of different application amounts of CaO2 on survival rates of rape seedlings under waterlogging condition
Fig. 2 Effects of different application amounts of CaO2 on fresh mass of rape seedlings under waterlogging conditionDifferent lowercase letters above bars indicate significant differences among different treatments at the same waterlogging time at the 0.05 probability level, and n=4. The same as Fig. 3.
Fig. 3 Effects of different application amounts of CaO2 on SPAD values of rape seedling leaves under waterlogging condition
Fig. 4 Effects of different application amounts of CaO2 on antioxidant enzyme activities in rape seedling leaves at waterlogging for 20 dDifferent lowercase letters above bars indicate significant differences at the 0.05 probability level, and n=4. The same as Figs. 5-6.
Fig. 5 Effects of different application amounts of CaO2 on fermentative enzyme activities in rape seedling roots at waterlogging for 20 d
Fig. 6 Effects of different application amounts of CaO2 on root activities of rape seedlings at waterlogging for 20 d
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