土壤类型对超积累植物东南景天叶际微生物群落结构和功能的影响

doi:10.3785/j.issn.1008-9209.2023.03.292

浙江大学学报（农业与生命科学版）

2024, Vol. 50

Issue (5): 758-770 DOI: 10.3785/j.issn.1008-9209.2023.03.292

资源利用与环境保护

土壤类型对超积累植物东南景天叶际微生物群落结构和功能的影响

姜悦(

),罗继鹏,乔亚蓓,李雨航,张雨,周润惠,李廷强(

)

浙江大学环境与资源学院，污染环境修复与生态健康教育部重点实验室，浙江杭州 310058

Effects of soil typeson phyllosphere microbial community structure and function of hyperaccumulator Sedum alfredii

Yue JIANG(

),Jipeng LUO,Yabei QIAO,Yuhang LI,Yu ZHANG,Runhui ZHOU,Tingqiang LI(

)

Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China

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摘要：

叶际微生物在促进植物生长、抵御生物和非生物胁迫中发挥重要作用。然而，目前对重金属超积累植物叶际微生物的群落结构和功能缺乏了解。本试验采用16S rRNA基因高通量测序，研究了红壤、紫色土、水稻土、青紫泥4种不同土壤类型对镉超积累生态型和非超积累生态型东南景天叶际微生物群落结构和功能的影响。结果表明：在青紫泥和水稻土中，东南景天叶际放线菌门和诺卡菌属微生物的相对丰度更高，而在红壤和紫色土中，东南景天叶际变形菌门、拟杆菌门、绿弯菌门微生物的相对丰度更高；在不同土壤中，超积累生态型东南景天叶际诺卡菌属、鞘氨醇单胞菌属和甲基杆菌属微生物的相对丰度均高于非超积累生态型。土壤类型和生态型显著影响东南景天叶际微生物的群落结构，且土壤类型的影响更大，解释了28.17%的群落变异。与青紫泥和紫色土相比，水稻土和红壤中叶际微生物的共存网络更为复杂；而所有土壤中超积累生态型叶际微生物的共存网络比非超积累生态型更为复杂。基于PICRUSt2软件的功能预测结果表明，土壤类型显著影响东南景天叶际微生物的谷胱甘肽代谢、D-谷氨酰胺和谷氨酸代谢、细菌趋化等通路的相对丰度（p＜0.01），而在不同土壤中，超积累生态型叶际微生物的碳水化合物代谢（柠檬酸循环）、氨基酸代谢（谷胱甘肽代谢、苯丙氨酸代谢、半胱氨酸和甲硫氨酸代谢）通路的相对丰度显著高于非超积累生态型（p＜0.01）。综上所述，土壤类型显著影响东南景天叶际微生物的群落结构和功能，且超积累生态型叶际微生物比非超积累生态型具有更强的镉胁迫抵抗能力。

关键词： 叶际微生物; 超积累植物; 共存网络; 镉; 植物修复

Abstract:

Phyllosphere microorganisms play important roles in promoting plant growth and resisting biotic and abiotic stresses. Nevertheless, there is a lack of knowledge on the phyllosphere microbial community structure and function of environment-specific plants, especially heavy metal-hyperaccumulating plants. In the present study, 16S rRNA high-throughput sequencing technology was used to explore the phyllosphere microbial community structure and function of the cadmium (Cd) hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) of Sedum alfredii grown in red soil, purple soil, paddy soil and purplish clayey soil. The results showed that in purplish clayey soil and paddy soil, the relative abundances of Actinobacteria and Nocardia microorganisms in the phyllosphere of S. alfredii were higher than those in red soil and purple soil, whereas the relative abundances of Proteobacteria, Bacteroidetes and Chloroflexi microorganisms in the phyllosphere of S. alfredii grown in red soil and purple soil were higher than those in purplish clayey soil and paddy soil. In different soils, the relative abundances of Nocardia, Sphingomonas and Methylobacterium microorganisms in HE were higher than those in NHE. The soil type and ecotype significantly affected the phyllosphere microbial community structure of S. alfredii, and the soil type was the greatest contributor, accounting for 28.17% of the variation. The co-occurrence networks of the phyllosphere microorganisms of S. alfredii grown in paddy soil and red soil were more complex than those in purplish clayey soil and purple soil. Moreover, compared with those in NHE, the co-occurrence networks in HE were more complex in all the soils. PICRUSt2-based functional prediction analysis showed that the soil type significantly affected the relative abundances of glutathione metabolism, D-glutamine and glutamate metabolism, and bacterial chemotaxis pathways in the phyllosphere microorganisms of S. alfredii (p＜0.01), whereas in different soils, the relative abundances of carbohydrate metabolism (citrate cycle) and amino acid metabolism (glutathione metabolism, phenylalanine metabolism, cysteine and methionine metabolism) pathways in the phyllosphere microorganisms of HE were significantly higher than those in NHE (p＜0.01). Taken together, the soil type significantly affects the structure and function of the phyllosphere microbial community of S. alfredii, and the phyllosphere microorganisms in HE are more resistant to Cd stress than those in NHE.

Key words: phyllosphere microorganisms hyperaccumulators co-occurrence network cadmium phytoremediation

收稿日期: 2023-03-29 出版日期: 2024-10-31

CLC:

Q938.1

基金资助: 国家自然科学基金项目(41977107);科技部中国-斯洛伐克2022—2023年度交流项目

通讯作者: 李廷强 E-mail: 22014121@zju.edu.cn;litq@zju.edu.cn

作者简介: 姜悦（https://orcid.org/0000-0003-4269-9932），E-mail：22014121@zju.edu.cn

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引用本文:

姜悦,罗继鹏,乔亚蓓,李雨航,张雨,周润惠,李廷强. 土壤类型对超积累植物东南景天叶际微生物群落结构和功能的影响[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(5): 758-770.

Yue JIANG,Jipeng LUO,Yabei QIAO,Yuhang LI,Yu ZHANG,Runhui ZHOU,Tingqiang LI. Effects of soil typeson phyllosphere microbial community structure and function of hyperaccumulator Sedum alfredii. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(5): 758-770.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.03.292 或 https://www.zjujournals.com/agr/CN/Y2024/V50/I5/758

表1 供试土壤的基本理化性质

表2 不同土壤中2种生态型东南景天地上部生物量 (g/pot)

表3 不同土壤中2种生态型东南景天叶片镉含量

图1 不同土壤中东南景天叶际微生物在门水平（A）和属水平（B）的群落组成

表4 不同土壤中2种生态型东南景天叶际微生物相对丰度的统计比较

图2 东南景天叶际微生物的群落结构分布（A）及各因素的差异贡献度（B）***表示在P＜0.001水平极显著相关。

图3 不同土壤中超积累生态型东南景天（A）和非超积累生态型东南景天（B）叶际特异微生物

图4 不同土壤中超积累生态型东南景天（A）和非超积累生态型东南景天（B）叶际微生物的共存网络特征红色边代表正相关关系，绿色边代表负相关关系。

图5 不同土壤中2种生态型东南景天叶际微生物群落的功能预测

表5 土壤类型和生态型对东南景天叶际微生物群落功能的影响

表6 不同土壤中2种生态型东南景天叶际微生物群落功能的统计比较

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