Experimental study on solidification parameters of Yellow River silt based on bacteria-induced and enzyme-induced methods

doi:10.3785/j.issn.1008-973X.2023.06.005

Journal of ZheJiang University (Engineering Science)

2023, Vol. 57

Issue (6): 1100-1110 DOI: 10.3785/j.issn.1008-973X.2023.06.005

Experimental study on solidification parameters of Yellow River silt based on bacteria-induced and enzyme-induced methods

Yu-ke WANG1(

),Tian-cai CAO1,Ying-bin SONG2,*(

),Jing-gan SHAO3,Xiang YU1,Bo-wen DONG4

1. College of Water Conservancy Engineering, Zhengzhou University, Zhengzhou 450001, China
2. Yellow River Institute of Hydraulic Research, Yellow River Conservancy Commission, Zhengzhou 450003, China
3. Henan Jiaoyuan Engineering Technology Limited Company, Zhengzhou 451460, China
4. Henan Communications Planning and Design Institute Limited Company, Zhengzhou 451450, China

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Abstract

In order to improve the quality of the Yellow River silt and popularize its application in subgrade engineering, the effects of microbially induced carbonate precipitation (MICP) and enzyme-induced carbonate precipitation (EICP) technology on the solidification of the Yellow River silt were compared and analyzed, based on the emerging biomineralization technology, considering the cement solution concentration and grouting times, using the unconfined compressive strength (UCS) test, mass fraction test of calcium carbonate and scanning electron microscope (SEM). Test results showed that the extraction efficiency of soybean urease was optimal when the mass concentration of soybean flour was 40 g/L. The highest activity of bacteria and soybean urease was obtained when the pH of the medium solution was 8. With a volume ratio of 1∶1 between the biological enzyme and the caking solution, urea reacted sufficiently with calcium chloride. The compressive strength of the Yellow River silt samples can be improved by increasing the cement solution concentration and the grouting times, but the influences of the two are different, and the cement solution concentration plays a leading role. The maximum compressive strength of the specimens cured by the EICP and MICP technology was 954.65 kPa and 674.98 kPa respectively, at the cement solution concentration of 1 mol/L and the grouting times of 8. The calcium carbonate generated in the Yellow River silt sample has a linear relationship with the compressive strength. The maximum mass fraction of calcium carbonate in the sample cured by EICP and MICP technology was 24.05% and 21.35% respectively. Calcite calcium carbonate is attached to the surface of the Yellow River silt particles and the pores between the particles, and the mass fraction of the calcium carbonate gradually decreases from top to bottom in the sample. Analysis of macroscopic and microscopic views indicates that the EICP is more suitable for solidifying the Yellow River silt than the MICP.

Key words： Yellow River silt microbially induced carbonate precipitation (MICP) enzyme-induced carbonate precipitation (EICP) compressive strength calcium carbonate

Received: 16 July 2022 Published: 30 June 2023

CLC:

TU 411

Fund: 国家自然科学基金资助项目(52178369，52109140，U2243222)；河南省自然科学基金资助项目 (202300410424)

Corresponding Authors: Ying-bin SONG E-mail: ykewang@163.com;2314231473@qq.com

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	Yu-ke WANG
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	Jing-gan SHAO
	Xiang YU
	Bo-wen DONG

Cite this article:

Yu-ke WANG,Tian-cai CAO,Ying-bin SONG,Jing-gan SHAO,Xiang YU,Bo-wen DONG. Experimental study on solidification parameters of Yellow River silt based on bacteria-induced and enzyme-induced methods. Journal of ZheJiang University (Engineering Science), 2023, 57(6): 1100-1110.

URL:

https://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2023.06.005 OR https://www.zjujournals.com/eng/Y2023/V57/I6/1100

基于菌促方法和酶促方法的黄河泥沙加固参数试验研究

为了改善黄河泥沙的质量和推广其在路基工程中的应用，基于新兴的生物矿化技术，考虑胶结液浓度和灌浆次数，采用无侧限抗压强度（UCS）试验、碳酸钙质量分数测试、扫描电镜（SEM）等方式，对比分析微生物诱导碳酸钙沉积（MICP）与酶诱导碳酸钙沉积（EICP）技术固化黄河泥沙试样的效果. 试验结果表明：大豆粉质量浓度为40 g/L时，大豆脲酶的提取效率最优；培养基溶液pH=8时，细菌和大豆脲酶的活性最好；生物酶和胶结液的体积比为1∶1时，尿素能够与氯化钙充分反应. 增加胶结液浓度、灌浆次数均能够提高黄河泥沙试样的抗压强度，但两者的影响规律不同，胶结液浓度起主导作用. 当胶结液浓度为1 mol/L、灌浆次数为8次时，EICP、MICP技术固化后试样的抗压强度达到最大，分别为954.65、674.98 kPa. 黄河泥沙试样内部生成的碳酸钙和抗压强度呈线性关系，EICP、MICP技术固化后试样的碳酸钙质量分数最大值分别为24.05%、21.35%. 黄河泥沙颗粒表面和颗粒之间的孔隙中均有方解石型碳酸钙附着，但其质量分数在试样内部自上而下逐渐降低. 宏观、微观的角度分析结果均表明，EICP技术相比MICP技术更加适合固化黄河泥沙.

关键词： 黄河泥沙, 微生物诱导碳酸钙沉积(MICP), 酶诱导碳酸钙沉积(EICP), 抗压强度, 碳酸钙

Fig.1 Grain gradation curve of Yellow River silt

Fig.2 Soybean urease solution

Fig.3 Soybean urease activity test

Fig.4 Bacterial solution with good activity

Fig.5 Prepared Yellow River silt samples

Fig.6 Grouting process of Yellow River silt samples

Tab.1 Test scheme for solidification of Yellow River silt

Fig.7 Pickling and water washing process of solidified Yellow River silt

Fig.8 Variation curves of soybean urease activity

Fig.9 Effect of pH on biological enzyme activity

Fig.10 Yellow River silt samples solidified by two technologies

Fig.11 Variation of compressive strength with binder concentration for samples treated with two technologies at different grouting times

Fig.12 Variation of compressive strength with grouting times for samples treated with two technologies at different binder concentrations

Fig.13 Three-dimensional compressive strength diagram of Yellow River silt sample under two solidification methods

Fig.14 Stress-strain curve of Yellow River silt sample

Fig.15 Damage of Yellow River silt samples

Fig.16 Three-dimensional mass fraction of calcium carbonate in samples solidified by EICP

Fig.17 Mass fraction of calcium carbonate in samples under two solidification methods

Fig.18 Relationship between mass fraction of calcium carbonate and compressive strength in samples solidified by ECIP

Fig.19 SEM image of Yellow River silt sample


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