Experimental study on improving effect of microorganism solidifying sand by ultrasonic

doi:10.3785/j.issn.1008-973X.2020.07.020

Journal of ZheJiang University (Engineering Science)

2020, Vol. 54

Issue (7): 1411-1417 DOI: 10.3785/j.issn.1008-973X.2020.07.020

Experimental study on improving effect of microorganism solidifying sand by ultrasonic

Zhi-ming LIU1,2(

),Jie PENG1,2,*(

),Jie LI1,2,En-run SONG3

1. Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering, Hohai University, Nanjing 210098, China
2. Jiangsu Research Center for Geotechnical Engineering Technology, Hohai University, Nanjing 210098, China
3. Design and Research Institute of the Second Construction Limited Company, China Construction Eighth Bureau, Jinan 250014, China

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Abstract

Orthogonal experiments of ultrasonic irradiation time (0-30 min) and ultrasonic power intensity (0-0.8 W/cm³) were conducted using ultrasound (20 kHz) in order to analyze ultrasonic effect on Sporosarcina pasteurii and its ability to induce calcium carbonate. Aqueous solution tests and sand column tests based on microbially induced carbonate precipitation were conducted using ultrasound-treated bacteria solution. Urease activity, OD₆₀₀, distribution parameters of bacterial cells, amount of microbially induced CaCO₃ and unconfined compressive strength of treated sand columns were measured. Effects of ultrasonic irradiation time, ultrasonic power intensity and ultrasonic energy intensity on CaCO₃ amount were analyzed. Results show that ultrasonic irradiation can trigger physiological enhancement reaction of bacteria and improve the urease activity of bacterial liquid. Ultrasonic irradiation can effectively improve the ability of bacteria to induce CaCO₃ with ultrasonic energy intensity of about 8 W·min/cm³. After bacterial solution was irradiated by ultrasound with the optimal irradiation strategy (ultrasonic power intensity of 0.4 W/cm³, treatment time of 20 minutes), CaCO₃ amount in aqueous solution tests and sand column tests can be increased by 28.5% and 35.6% respectively. Unconfined compressive strength of MICP-treated sand sample was 1.25 MPa, which was increased by 91.6% than control samples.

Key words： microbially induced carbonate precipitation (MICP) ultrasound urease activity precipitation amount of CaCO₃ irradiation time ultrasonic energy intensity

Received: 05 June 2019 Published: 05 July 2020

CLC:

TU 52

Corresponding Authors: Jie PENG E-mail: lzm@hhu.edu.cn;peng-jie@hhu.edu.cn

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Cite this article:

Zhi-ming LIU,Jie PENG,Jie LI,En-run SONG. Experimental study on improving effect of microorganism solidifying sand by ultrasonic. Journal of ZheJiang University (Engineering Science), 2020, 54(7): 1411-1417.

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http://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2020.07.020 OR http://www.zjujournals.com/eng/Y2020/V54/I7/1411

超声波提高微生物固化砂土效果的试验研究

为了研究超声波对巴氏生孢八叠球菌及其诱导碳酸钙沉积能力的影响，利用超声（20 kHz）开展超声辐照时间（0~30 min）和超声功率强度（0~0.8 W/cm³）的正交试验，用超声处理后的菌液进行水溶液试验和砂柱加固试验. 测定菌液脲酶活性、OD₆₀₀、细菌颗粒分布参数、碳酸钙的产量和加固后砂柱无侧限抗压强度. 分析超声辐照时间、超声功率强度、超声能量强度对碳酸钙产量的影响. 结果表明，超声辐照能够引发细菌产生生理强化反应，提高菌液脲酶活性. 当超声能量强度约为8 W·min/cm³时，超声辐照能够有效地提高菌液诱导生成碳酸钙的能力. 经优化的辐照策略（超声功率强度为0.4 W/cm³，处理时间为20 min）进行超声辐照处理后，水溶液中和砂柱中的碳酸钙产量分别提高了28.5%和35.6%，加固后砂样无侧限抗压强度为1.25 MPa，较对照组提高了91.6%.

关键词： 微生物诱导碳酸钙沉积（MICP）, 超声波, 脲酶活性, 碳酸钙产量, 辐照时间, 超声能量强度

Fig.1 Particle size distribution of standard sand

Tab.1 Design of ultrasonic irradiation treatment tests

Fig.2 Particle distribution parameters of bacterial solution

Fig.3 Relationship between OD₆₀₀ of bacterial solution and ultrasonic power intensity

Fig.4 Relationship between urease activity parameters and irradiation time

Fig.5 CaCO₃ production in aqueous solution tests

Tab.2 Ultrasonic energy intensity of each test

Fig.6 Urease activity and OD₆₀₀ varied with ultrasonic energy intensity

Fig.7 Relationship between CaCO₃ content and ultrasonic energy intensity

Fig.8 Ultrasonic power intensity and proportion of bacterial activity of bacteaial liquid used in sand column treatment tests

Fig.9 Results of sand column tests


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