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浙江大学学报(工学版)
浙江大学学报(工学版)  2025, Vol. 59 Issue (8): 1590-1597    DOI: 10.3785/j.issn.1008-973X.2025.08.005
机械工程、能源工程     
基于柔性印刷微电极阵列的心肌场电位与传导监测
方灏航1(),刘玲玲1,江阿灿1,许丰1,张长瑞1,金航1,高强2,林彬2,3,陈松月1,*(),孙道恒1
1. 厦门大学 萨本栋微米纳米科学技术研究院，福建 厦门 361102
2. 广东省人民医院，广东 广州 510080
3. 广东源心再生医学有限公司，广东 佛山 528231
Myocardial field potential and conduction monitoring based on flexible printed microelectrode array
Haohang FANG1(),Lingling LIU1,Acan JIANG1,Feng XU1,Changrui ZHANG1,Hang JIN1,Qiang GAO2,Bin LIN2,3,Songyue CHEN1,*(),Daoheng SUN1
1. Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361102, China
2. Guangdong Provincial People’s Hospital, Guangzhou 510080, China
3. Guangdong Beating Origin Regenerative Medicine Limited Company, Foshan 528231, China
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摘要：

为了构建适用于心肌组织场电位监测与药物筛选的平台，搭建基于柔性印刷的微电极阵列的平台，用于监测人源心肌组织的电生理活动和兴奋传导. 利用同步采集功能，从16个工作电极采集心肌组织的场电位信号. 对心肌组织兴奋传导的分析揭示了心肌组织的同步性和节律性收缩. 通过异丙肾上腺素和维拉帕米的测试，证明了该平台在药物筛选中的应用潜力，这些药物显著影响了心肌组织的收缩频率、场电位幅度与场电位持续时间.
关键词： 微电极阵列;  兴奋传导;  电生理活动;  监测平台;  药物测试    
Abstract:

A flexible printed microelectrode array (MEA) system was constructed to develop a platform suitable for field potential monitoring and drug screening of cardiac tissue. The platform was designed to record the electrophysiological activity and excitation conduction of human-derived cardiac tissue. Field potential signals were collected from 16 working electrodes by using a synchronous acquisition function. The analysis of excitation conduction revealed synchronized and rhythmic contractions within the tissue. Drug testing with isoproterenol and verapamil demonstrated the platform’s potential for pharmacological evaluation. These drugs significantly altered contraction frequency, field potential amplitude, and duration.
Key words: microelectrode array    excitation conduction    electrophysiological activity    monitoring platform    drug test
收稿日期: 2024-07-11 出版日期: 2025-07-28
:  TP 212  
基金资助: 国家自然科学基金资助项目（U2005214）；中国科技部国家重点研发计划资助项目（2022YFB4600600）；翔安创新实验室/传染病疫苗研发全国重点实验室科技资助项目（2023XAKJ0103064）.
通讯作者: 陈松月     E-mail: fanghaohang@stu.xmu.edu.cn;s.chen@xmu.edu.cn
作者简介: 方灏航（1999—），男，硕士生，从事心肌组织芯片的研究. orcid.org/0009-0002-7627-2099. E-mail: fanghaohang@stu.xmu.edu.cn
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方灏航
刘玲玲
江阿灿
许丰
张长瑞
金航
高强
林彬
陈松月
孙道恒

引用本文:

方灏航,刘玲玲,江阿灿,许丰,张长瑞,金航,高强,林彬,陈松月,孙道恒. 基于柔性印刷微电极阵列的心肌场电位与传导监测[J]. 浙江大学学报(工学版), 2025, 59(8): 1590-1597.

Haohang FANG,Lingling LIU,Acan JIANG,Feng XU,Changrui ZHANG,Hang JIN,Qiang GAO,Bin LIN,Songyue CHEN,Daoheng SUN. Myocardial field potential and conduction monitoring based on flexible printed microelectrode array. Journal of ZheJiang University (Engineering Science), 2025, 59(8): 1590-1597.

链接本文:

https://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2025.08.005    或    https://www.zjujournals.com/eng/CN/Y2025/V59/I8/1590

图 1  MEA的设计、工艺与生物相容性验证
图 2  电生理检测平台的搭建
图 3  电生理检测平台采集系统的测试示意及性能评估
图 4  心肌组织兴奋传导监测
图 5  不同浓度异丙肾上腺素作用下的心肌组织场电位
图 6  不同浓度维拉帕米作用下的心肌组织场电位
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