Identification and expression analysis of myo-inositol oxygenase gene family in <i>Brassica napus</i> L<i>.</i>

doi:10.3785/j.issn.1008-9209.2022.09.281

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (4): 484-496 DOI: 10.3785/j.issn.1008-9209.2022.09.281

Research articles

Identification and expression analysis of myo-inositol oxygenase gene family in Brassica napus L.

Shubing CHEN¹(

),XU Zishu¹,Qian HUANG²,Hui ZHANG³,Kangni ZHANG²,Yi DUAN¹,Yue’e SUN¹,Weijun ZHOU²,Ling XU¹(

)

^1.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China
^2.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
^3.Zhejiang Agricultural Technology Extension Center, Hangzhou 310020, Zhejiang, China

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Abstract

Myo-inositol oxygenase (MIOX) catalyzes the conversion of myo-inositol to glucuronic acid and plays an important role in response to biotic and abiotic stresses. In this study, the genome-wide identification and expression pattern analysis of the MIOX family gene in Brassica napus L.(BnMIOX) were conducted. The results showed that the BnMIOX family included 12 members distributed across nine chromosomes. According to the characteristics of MIOX gene domain, the phylogenetic tree of B. napus, Arabidopsisthaliana, B. rapa and B. oleracea could be divided into subfamilies Ⅰ, Ⅱ and Ⅲ. No tandem repeat gene pairs were found in the collinearity analysis, and all of them were large segment replication genes, demonstrating that segmental duplication and whole-genome duplication were the main driving forces for the MIOX gene family amplification in B. napus. The transcriptomic data indicated that BnMIOX genes showed different temporal and spatial expression patterns in different tissues and different growth and development processes. Expression profiles under different stresses demonstrated that the expression of BnMIOX1 gene was obviously induced under drought and salt stresses, while BnMIOX1, BnMIOX2, and BnMIOX9 genes had significant responses to drought, salt, abscisic acid (ABA), and cold stresses. The results of the protein interaction network analysis further showed that the BnMIOX interacted with proteins including GLCAK, PIS1, VTC2, VTC4, and PDF2.1, implying that BnMIOX genes play key roles in improving the resistance of B. napus. This study provides an important basis for further investigation of the function of BnMIOX genes.

Key words： Brassica napus L. myo-inositol oxygenase (MIOX) gene family abiotic stress gene expression

Received: 28 September 2022 Published: 29 August 2023

CLC:

S565.4

Corresponding Authors: Ling XU E-mail: 2864928337@qq.com;lxu@zstu.edu.cn

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	Shubing CHEN
	XU Zishu
	Qian HUANG
	Hui ZHANG
	Kangni ZHANG
	Yi DUAN
	Yue’e SUN
	Weijun ZHOU
	Ling XU

Cite this article:

Shubing CHEN,XU Zishu,Qian HUANG,Hui ZHANG,Kangni ZHANG,Yi DUAN,Yue’e SUN,Weijun ZHOU,Ling XU. Identification and expression analysis of myo-inositol oxygenase gene family in Brassica napus L.. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(4): 484-496.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.09.281 OR https://www.zjujournals.com/agr/Y2023/V49/I4/484

甘蓝型油菜肌醇加氧酶基因家族鉴定与表达分析

肌醇加氧酶（myo-inositol oxygenase, MIOX）催化肌醇转化为葡糖醛酸，在响应生物和非生物胁迫中发挥着重要作用。本研究对甘蓝型油菜MIOX家族基因（BnMIOX）进行了全基因组鉴定和表达模式分析。结果表明，BnMIOX家族包括12个成员，分布在9条染色体上；根据MIOX基因结构域特点，可以将甘蓝型油菜与拟南芥、白菜和甘蓝构建的系统进化树分为Ⅰ、Ⅱ、Ⅲ3个亚家族；且共线性分析中没有找到串联重复基因对，全部为大片段复制基因，表明片段重复和全基因组重复是甘蓝型油菜MIOX基因家族扩增的主要驱动力。转录组数据分析表明，BnMIOX基因在不同组织、不同生长发育过程中的时空表达模式不同。不同胁迫下的表达谱分析表明，BnMIOX1基因在干旱、盐胁迫下被显著诱导表达，且BnMIOX1、BnMIOX2和BnMIOX9基因对干旱、盐、脱落酸（abscisic acid, ABA）和冷胁迫响应较为明显。蛋白质互作网络分析结果进一步表明，BnMIOX与GLCAK、PIS1、VTC2、VTC4、PDF2.1等蛋白存在互作，推测BnMIOX基因在提高甘蓝型油菜抗性过程中发挥关键作用。本研究为进一步探讨BnMIOX基因的功能提供了依据。

关键词： 甘蓝型油菜, 肌醇加氧酶, 基因家族, 非生物胁迫, 基因表达

Table 1 Sequence characteristics of the BnMIOX family

Fig. 1 Phylogenetic tree of MIOX protein sequences in B. napus, A. thaliana, B. rapa and B. oleracea

represents A. thaliana;

represents B. oleracea;

represents B. rapa;

represents B. napus.

Fig. 3 Predicted cis-acting elements in the BnMIOX promoters

Table 2 Information for predicted partial cis-acting elements

Fig. 2 Gene structures and conserved motifs ofBnMIOX familyA. Gene structures of BnMIOX family; B. Conserved motifs of BnMIOX family.

Fig. 4 Distribution of the BnMIOX genes on chromosomesEach chromosome is divided by 0.1 Mb, and the color in this region represents the gene density (blue: low; white: medium; red: high).

Fig. 5 Collinearity analysis of the BnMIOX familyGray regions indicate all collinearity regions in the B. napus genome, and yellow lines indicate replication events of the BnMIOX genes.

Fig. 6 Collinearity analysis of the MIOX genes among different plantsGray lines indicate the collinearity of B. napus with all genes of other species, and red lines indicate MIOX gene pairs with collinear relationships.

Fig. 7 Heatmap of BnMIOX gene expression in different tissues and organsFPKM: Fragments per kilobase of exon model per million mapped reads.

Fig. 8 Heatmap of BnMIOX gene expression under different stresses

Table 3 Root mean square deviation (RMSD) between predicted structures of BnMIOX proteins

Fig. 9 Three-dimensional structures of BnMIOX proteinsThe structural model is the crystal structure of mouse myo-inositol oxygenase in complex with substrate.

Fig. 10 Prediction of interaction networks between BnMIOX protein and the other proteinThe circles represent all kinds of proteins, and the connecting line represents protein-protein interaction.


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