Effects of spermine on immune organ indexes and expression levels of genes related to immune factors in geese

doi:10.3785/j.issn.1008-9209.2018.10.252

Journal of Zhejiang University (Agriculture and Life Sciences)

2019, Vol. 45

Issue (5): 596-602 DOI: 10.3785/j.issn.1008-9209.2018.10.252

Animal sciences & veterinary medicine

Effects of spermine on immune organ indexes and expression levels of genes related to immune factors in geese

Zhixin YI^1,²(

),Yilong JIANG¹,Qiuhong WANG¹,Qilin XU³,Xinxing WANG¹,Guilin MO¹,Dongmei JIANG¹,Bo KANG¹(

)

1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
2. Bazhong Academy of Agriculture and Forestry Sciences, Bazhong 636000, Sichuan, China
3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China

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Abstract

To investigate the effects of spermine on goose immune organ indexes and expression levels of genes related to immune factors, the Sichuan adult white geese (female) were intragastrically administered with 5 and 10 mg spermine per kilogram body mass, and then the indexes of liver, thymus, spleen and bursa of Fabricius were measured. In addition, the expression levels of TNF-α, IL-1β, IL-6 and IFN-γ genes and polyamine contents in the liver, thymus, spleen and bursa of Fabricius were determined. The control group was intragastrically administered with physiological saline. The results showed that the liver index in the geese treated with 10 mg/kg spermine was significantly lower than the control group (P＜0.05). The expression levels of TNF-α, IL-1β, IL-6 and IFN-γ genes in the thymus and bursa of Fabricius of geese treated with 5 mg/kg spermine were significantly lower than the control group (P＜0.05), respectively. The expression levels of TNF-α and IFN-γ in the bursa of Fabricius and IFN-γ in the liver of geese treated with 10 mg/kg spermine were significantly lower than the control group (P＜0.05), respectively. The expression level of IFN-γ was significantly decreased in the spleen by the treatments of spermine compared with the control group (P＜0.05). The contents of spermine in the liver of geese treated with spermine were significantly greater than the control group (P＜0.05). In summary, these results suggest that spermine regulates the immunologic function of geese by mediating the expression levels of genes related to immune factors with the tissue-specific and dose-dependent manner.

Key words： spermine goose immune organ index immune factor gene expression

Received: 25 October 2018 Published: 05 December 2019

CLC:

S 835

Corresponding Authors: Bo KANG E-mail: yizhixin926@163.com;bokang@sicau.edu.cn

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	Zhixin YI
	Yilong JIANG
	Qiuhong WANG
	Qilin XU
	Xinxing WANG
	Guilin MO
	Dongmei JIANG
	Bo KANG

Cite this article:

Zhixin YI,Yilong JIANG,Qiuhong WANG,Qilin XU,Xinxing WANG,Guilin MO,Dongmei JIANG,Bo KANG. Effects of spermine on immune organ indexes and expression levels of genes related to immune factors in geese. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(5): 596-602.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2018.10.252 OR http://www.zjujournals.com/agr/Y2019/V45/I5/596

精胺对鹅免疫器官指数及免疫相关因子基因表达的影响

为研究精胺对鹅免疫器官指数及免疫相关因子基因表达的影响，用5和10 mg/kg精胺灌喂成年四川白鹅雌鹅，测定其肝指数、胸腺指数、脾指数和法氏囊指数，检测在各免疫器官组织中TNF-α、IL-1β、IL-6和IFN-γ基因表达量和多胺含量；对照组灌喂禽用生理盐水。结果表明：10 mg/kg精胺灌喂组的鹅肝指数显著低于对照组（P＜0.05）；5 mg/kg精胺灌喂组的鹅胸腺和法氏囊中TNF-α、IL-1β、IL-6和IFN-γ表达量均显著低于对照组（P＜0.05）；10 mg/kg精胺灌喂组的鹅法氏囊中TNF-α和IFN-γ及肝中IFN-γ表达量均显著低于对照组（P＜0.05）；外源性精胺显著下调脾中IFN-γ表达量（P＜0.05）；精胺灌喂组的鹅肝内精胺含量显著高于对照组（P＜0.05）。说明精胺可通过介导鹅免疫相关因子基因表达来参与调控鹅免疫功能，并且存在剂量依赖性和组织特异性。

关键词： 精胺, 鹅, 免疫器官指数, 免疫因子, 基因表达

Table 1 List of primer sequences

Fig. 1 Electrophoretogram of total RNA in the immune organ of female geese1: Liver; 2: Thymus; 3: Spleen; 4 Bursa of Fabricius.

Fig. 2 Electrophoretogram of RT-PCR products for target genes in the liver of female geeseM: DL2000 marker.

Fig. 3 Melt curves of real-time quantitative PCR amplifica-tions of target genes－d (RFU)/dT: The negative first derivative of the relative fluo-rescence units.

Fig. 4 Effects of spermine on immune organ indexes of the liver, thymus, spleen and bursa of Fabricius in female geeseDifferent lowercase letters above the bars show significant differences among different treatments at the 0.05 probability level, and no letters above the bars show no significant differences at the 0.05 probability level; n=6.

Fig. 5 Effects of spermine on expression levels of genes related to immune factors in female geeseDifferent lowercase letters above the bars show significant differences among different treatments at the 0.05 probability level, and no letters above the bars show no significant differences at the 0.05 probability level; n=6.

Fig. 6 Effects of spermine on polyamine contents in the liver, thymus, spleen and bursa of Fabricius of female geeseDifferent lowercase letters above the bars show significant differences among different treatments at the 0.05 probability level, and no letters above the bars show no significant differences at the 0.05 probability level; n=6.


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