Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale

doi:10.3785/j.issn.1008-9209.2020.10.221

Journal of Zhejiang University (Agriculture and Life Sciences)

2021, Vol. 47

Issue (3): 314-324 DOI: 10.3785/j.issn.1008-9209.2020.10.221

Horticulture

Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale

Youxuan WANG¹(

),Mengyu WANG¹,Yubo LI¹,Han TAO¹,Chuchu XIA¹,Kaimei HUANG²(

),Qiaomei WANG¹(

)

^1.Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University/Ministry of Agriculture and Rural Affairs Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Hangzhou 310058, China
^2.Hangzhou Agricultural Technology Extension Center, Hangzhou 310017, China

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Abstract

To better understand the indole-3-acetic acid (IAA) response pathway and explore thespecific functions of Aux/IAA gene family in the growth of Chinese kale and in response to salt stress, forty-one Aux/IAA genes were collected and analyzed by bioinformatics methods from Chinese kale genome. Phylogenetic analysis showed that the Aux/IAA genes of Chinese kale were distributed on nine chromosomes and the most genes were distributed on chromosome C01 and C05 including six members, respectively. The number of the amino acid residues of proteins encoded by Aux/IAA genes ranged from 128 to 427, and most of them were hydrophilic proteins, and only two were hydrophobic proteins. The subcellular localization analysis indicated that the Aux/IAA family members were mostly located in cytoplasm, nucleus and chloroplast. The results of real-time quantitative polymerase chain reaction (qRT-PCR) showed that several Aux/IAA genes of Chinese kale responded to IAA, abscisic acid (ABA) and brassinosteroids (BR) treatments, which suggested that the Aux/IAA gene family was probably involved in plant hormone signal transduction process. Additionally, under salt stress treatment, the expression of BoIAA19-1 and BoIAA2-1 genes in Chinese kale were significantly up-regulated during the early treatment stage, indicating that Aux/IAA genes may also play roles in response to diverse environmental stresses. The results of this research lay theoretical foundations for further exploring the function of the Aux/IAA gene family in the growth and resistance balance of Chinese kale.

Key words： Chinese kale Aux/IAA gene family bioinformatics analysis expression analysis

Received: 22 October 2020 Published: 25 June 2021

CLC:

S 635.9

Corresponding Authors: Kaimei HUANG,Qiaomei WANG E-mail: 3170100567@zju.edu.cn;haungkm@163.com;qmwang@zju.edu.cn

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Cite this article:

Youxuan WANG,Mengyu WANG,Yubo LI,Han TAO,Chuchu XIA,Kaimei HUANG,Qiaomei WANG. Bioinformatics and expression analysis of Aux/IAA family gene in Chinese kale. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(3): 314-324.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.10.221 OR http://www.zjujournals.com/agr/Y2021/V47/I3/314

芥蓝Aux/IAA家族基因生物信息学与表达分析

为更好地理解芥蓝生长素（indole-3-acetic acid, IAA）的响应途径及揭示Aux/IAA基因家族在芥蓝生长和抗盐胁迫中的功能，本研究利用生物信息学的方法从芥蓝基因组中鉴定到了41个Aux/IAA家族基因。系统进化分析结果表明，芥蓝Aux/IAA基因家族成员分布于9条染色体上，在染色体C01和C05上分布的基因最多，各有6个；该家族基因编码的蛋白质的氨基酸残基数在128～427之间，大多数为亲水性蛋白，仅有2个疏水性蛋白。亚细胞定位预测结果表明，Aux/IAA家族成员多位于细胞质、细胞核和叶绿体上。实时荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction, qRT-PCR）结果表明，芥蓝Aux/IAA基因家族部分成员对生长素（IAA）、脱落酸（abscisic acid, ABA）、油菜素内酯（brassinosteroids, BR）处理存在不同程度的响应，说明芥蓝Aux/IAA基因家族很可能参与植物激素信号的传导。此外，经过盐胁迫处理后，芥蓝BoIAA19-1与BoIAA2-1的表达量在处理前期均显著上调，预示Aux/IAA基因在芥蓝响应逆境的过程中也可能发挥作用。本研究结果为进一步研究Aux/IAA基因家族在芥蓝生长和抗性平衡中的功能奠定了理论基础。

关键词： 芥蓝, Aux/IAA基因家族, 生物信息学分析, 表达分析

Table 1 Primers used for qRT-PCR

序列编码 Sequence ID	基因 Gene	氨基酸数目 Amino acid number	分子质量 Molecular mass/ Da	等电点 pI	不稳定系数 Instability index	脂肪指数 Aliphatic index	平均亲水系数 Average hydrophilic index
＞Bol038615	BoIAA2-1	191	21 622.69	5.81	52.33	60.63	－0.713
＞Bol028707	BoIAA2-2	198	22 194.54	8.70	41.01	71.41	－0.554
＞Bol040875	BoIAA3-1	186	21 022.09	7.48	42.37	65.91	－0.560
＞Bol011602	BoIAA3-2	178	18 945.90	4.38	18.17	123.31	0.778
＞Bol009531	BoIAA4-1	191	21 622.69	5.81	52.33	60.63	－0.713
＞Bol009871	BoIAA5-1	128	14 445.81	7.63	57.12	86.72	－0.266
＞Bol039162	BoIAA6-1	203	22 637.07	7.62	34.75	89.66	0.299
＞Bol031746	BoIAA8-1	271	29 842.94	8.57	38.33	73.73	－0.500
＞Bol004361	BoIAA9-1	329	35 540.51	8.36	40.21	76.28	－0.385
＞Bol008065	BoIAA9-2	293	31 658.86	6.63	37.90	71.23	－0.400
＞Bol040868	BoIAA10-1	777	83 370.16	9.34	40.60	87.04	－0.087
＞Bol019649	BoIAA11-1	245	26 415.58	5.47	43.92	72.04	－0.473
＞Bol033544	BoIAA11-2	380	41 675.44	6.18	38.16	74.34	－0.339
＞Bol040903	BoIAA12-1	230	25 408.79	9.57	39.78	64.43	－0.770
＞Bol018343	BoIAA12-2	427	46 214.79	9.45	35.67	81.80	－0.277
＞Bol027258	BoIAA13-1	236	25 453.83	9.20	31.29	71.10	－0.555
＞Bol037015	BoIAA13-2	247	26 849.49	9.35	33.26	70.65	－0.582
＞Bol001436	BoIAA15-1	177	20 113.24	7.62	49.15	81.47	－0.264
＞Bol002707	BoIAA16-1	277	30 267.84	9.30	28.07	76.35	－0.374
＞Bol003120	BoIAA16-2	231	25 139.77	8.64	31.57	69.26	－0.400
＞Bol019495	BoIAA18-1	253	28 219.75	9.61	45.10	62.37	－0.911
＞Bol002014	BoIAA18-2	224	25 006.05	9.73	50.50	59.73	－0.745
＞Bol011172	BoIAA19-1	177	19 448.22	5.95	47.76	70.40	－0.441
＞Bol034711	BoIAA19-2	197	21 826.94	5.23	43.17	72.59	－0.393
＞Bol021812	BoIAA20-1	174	19 325.41	4.77	48.03	65.52	－0.438
＞Bol036925	BoIAA26-1	268	30 106.10	9.04	48.65	65.49	－0.894
＞Bol034783	BoIAA26-2	236	26 472.57	6.61	42.55	65.21	－0.967
＞Bol019603	BoIAA27-1	275	30 134.07	8.73	49.02	63.13	－0.599
＞Bol021102	BoIAA27-2	230	25 273.68	8.41	47.57	65.70	－0.511
＞Bol022320	BoIAA28-1	180	20 905.83	9.12	52.73	80.00	－0.653
＞Bol032479	BoIAA28-2	175	20 024.79	8.99	44.03	80.63	－0.679
＞Bol017925	BoIAA29-1	191	22 181.01	5.88	44.78	65.81	－0.705
＞Bol033768	BoIAA29-2	191	22 006.65	5.22	49.78	64.76	－0.646
＞Bol045689	BoIAA30-1	177	20 123.18	4.73	70.69	62.77	－0.634
＞Bol014397	BoIAA30-2	172	19 376.50	4.90	68.62	70.23	－0.477
＞Bol038661	BoIAA31-1	128	14 329.09	9.33	34.88	73.05	－0.586
＞Bol022926	BoIAA31-2	157	18 134.68	8.36	46.15	75.03	－0.527
＞Bol007955	BoIAA32-1	188	21 434.09	7.78	29.40	73.56	－0.634
＞Bol012219	BoIAA33-1	174	19 128.98	4.57	37.66	72.24	－0.502
＞Bol014224	BoIAA33-2	175	19 575.91	6.97	43.88	75.71	－0.603
＞Bol038104	BoIAA34-1	164	19 037.51	6.60	25.41	68.78	－0.668

Table 2 Information and physicochemical properties of Aux/IAA family in Chinese kale

Fig. 1 Chromosome mapping of the Aux/IAA gene family in Chinese kale

Fig. 2 Phylogenetic tree analysis of Aux/IAA family protein in Chinese kale

Fig. 3 Analysis of protein conserved sequence motif ofAux/IAA family in Chinese kale

Fig. 4 Structures of Aux/IAA proteins’ conserved domain Ⅰ, Ⅱ, Ⅲ and Ⅳ

Fig. 5 Expressions of some Aux/IAA gene family members under different hormone treatmentsCK: 0 h, clear water treatment.

Fig. 6 Expressions of some Aux/IAA gene family members under salt stress treatmentsCK: 0 h, clear water treatment. Single asterisk (*) indicates significant differences at the 0.05 probability level as compared with the control group (CK).


[1]	FARCOT E, LAVEDRINE C, VERNOUX T. A modular analysis of the auxin signalling network. PLoS ONE, 2015,10(6):e0122231. DOI:10.1371/journal.pone.0122231 doi: 10.1371/journal.pone.0122231

[2]	李小平,曾庆发,张根生,等.大豆生长素响应因子基因GmARF17对胚轴生长发育的调控作用.核农学报,2015,29(9):1668-1676. DOI:10.11869/j.issn.100-8551.2015.09.1668 LI X P, ZENG Q F, ZHANG G S, et al. The regulation effect of the soybean auxin response factor gene GmARF17 on the growth and development of hypocotyls. Journal of Nuclear Agricultural Sciences, 2015,29(9):1668-1676. (in Chinese with English abstract) doi: 10.11869/j.issn.100-8551.2015.09.1668

[3]	YAMAMOTO M, YAMAMOTO K T. Differential effects of 1-naphthaleneacetic acid, indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid on the gravitropic response of roots in an auxin-resistant mutant of Arabidopsis, aux1. Plant and Cell Physiology, 1998,39(6):660-664.

[4]	鄂志国,王磊.水稻中生长素作用的分子机理研究进展.核农学报,2011,25(4):730-735. E Z G, WANG L. Advances on molecular mechanism of auxin in rice. Journal of Nuclear Agricultural Sciences, 2011,25(4):730-735. (in Chinese with English abstract)

[5]	SZEMENYEI H, HANNON M, LONG J A. TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis. Science, 2008,319(5868):1384-1386. DOI:10.1126/science.1151461 doi: 10.1126/science.1151461

[6]	WEIJERS D, WAGNER D. Transcriptional responses to the auxin hormone. Annual Review of Plant Biology, 2016,67:539-574. DOI:10.1146/annurev-arplant-043015-112122 doi: 10.1146/annurev-arplant-043015-112122

[7]	SALEHIN M, LI B H, TANG M, et al. Auxin-sensitive Aux/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels. Nature Communications, 2019,10:4021. DOI:10.1038/s41467-019-12002-1 doi: 10.1038/s41467-019-12002-1

[8]	WANG J, YAN D W, YUAN T T, et al. A gain-of-function mutation in IAA8 alters Arabidopsis floral organ development by change of jasmonic acid level. Plant Molecular Biology, 2013,82(1/2):71-83. DOI:10.1007/s11103-013-0039-y doi: 10.1007/s11103-013-0039-y

[9]	HU J H, ISRAELI A, ORI N, et al. The interaction between DELLA and ARF/IAA mediates crosstalk between gibberellin and auxin signaling to control fruit initiation in tomato. The Plant Cell, 2018,30(8):1710-1728. DOI:10.1105/tpc.18.00363 doi: 10.1105/tpc.18.00363

[10]	GUILLOTIN B, ETEMADI M, AUDRAN C, et al. Sl-IAA27 regulates strigolactone biosynthesis and mycorrhization in tomato (var. MicroTom). New Phytologist, 2017,213:1124-1132. DOI:10.1111/nph.14246 doi: 10.1111/nph.14246

[11]	BEDNAREK P, PI?LEWSKA-BEDNAREK M, SVATO? A, et al. A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science, 2009,323(5910):101-106. DOI:10.1126/science.1163732 doi: 10.1126/science.1163732

[12]	曾文芳,王小贝,潘磊,等.桃Aux/IAA家族基因鉴定及在果实成熟过程中的表达分析.园艺学报,2017,44(2):233-244. DOI:10.16420/j.issn.0513-353x.2016-0388 ZENG W F, WANG X B, PAN L, et al. Identification and expression profiling of Aux/IAA family gene during peach fruit ripening. Acta Horticulturae Sinica, 2017,44(2):233-244. (in Chinese with English abstract) doi: 10.16420/j.issn.0513-353x.2016-0388

[13]	田爱梅,于晖.芥蓝BaCSLD的克隆、亚细胞定位及表达分析.核农学报,2020,34(5):948-953. DOI:10.11869/j.issn.100-8551.2020.05.0948 TIAN A M, YU H. Cloning, subcelluar localization and expression analysis of BaCSLD from Brassica alboglabra. Journal of Nuclear Agricultural Sciences, 2020,34(5):948-953. (in Chinese with English abstract) doi: 10.11869/j.issn.100-8551.2020.05.0948

[14]	卢世雄,王萍,何红红,等.葡萄Trihelix转录因子家族生物信息及其基因表达分析.园艺学报,2019,46(7):1257-1269. DOI:10.5784/27-2-100-10 LU S X, WANG P, HE H H, et al. Bioinformatics identification and expression analysis of grape Trihelix transcription factor family. Acta Horticulturae Sinica, 2019,46(7):1257-1269. (in Chinese with English abstract) doi: 10.5784/27-2-100-10

[15]	LEYSER O. Auxin signaling. Plant Physiology, 2018,176(1):465-479. DOI:10.1104/pp.17.00765 doi: 10.1104/pp.17.00765

[16]	GUILFOYLE T J, HAGEN G. Getting a grasp on domain Ⅲ/Ⅳ responsible for auxin response factor-IAA protein interactions. Plant Science, 2012,190:82-88. DOI:10.1016/j.plantsci.2012.04.003 doi: 10.1016/j.plantsci.2012.04.003

[17]	WALKER J C, KEY J L. Isolation of cloned cDNAS to auxin-responsive poly(A)＋RNAs of elongating soybean hypocotyl. PNAS, 1982,79:7185-7189.

[18]	OVERVOORDE P J, OKUSHIMA Y, ALONSO J M, et al. Functional genomic analysis of the Auxin/Indole-3-AceticAcid gene family members in Arabidopsis thaliana. The Plant Cell, 2005,17(12):3282-3300. DOI:10.1105/tpc.105.036723 doi: 10.1105/tpc.105.036723

[19]	WANG Y J, DENG D X, BIAN Y L, et al. Genome-wide analysis of primary auxin-responsive Aux/IAA gene family in maize (Zea mays L.). Molecular Biology Reports, 2010,37(8):3991-4001. DOI:10.1007/s11033-010-0058-6 doi: 10.1007/s11033-010-0058-6

[20]	QIAO L Y, ZHANG X J, HAN X, et al. A genome-wide analysis of the Auxin/indole-3-aceticacid gene family in hexaploid bread wheat (Triticum aestivum L.). Frontiers in Plant Science, 2015,6:770. DOI:10.3389/fpls.2015.00770 doi: 10.3389/fpls.2015.00770

[21]	SINGH V K, JAIN M. Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean. Frontiers in Plant Science, 2015,6:918. DOI:10.3389/fpls.2015.00918 doi: 10.3389/fpls.2015.00918

[22]	WU J, PENG Z, LIU S Y, et al. Genome-wide analysis of Aux/IAA gene family in Solanaceae species using tomato as a model. Molecular Genetics and Genomics, 2012,287(4):295-311. DOI:10.1007/s00438-012-0675-y doi: 10.1007/s00438-012-0675-y

[23]	方智振,姜翠翠,周丹蓉,等.三月李及其红肉突变体Aux/IAA家族基因鉴定及分析.核农学报,2020,34(2):247-255. DOI:10.11869/j.issn.100-8551.2020.02.0247 FANG Z Z, JIANG C C, ZHOU D R, et al. Identification and analysis of Aux/IAA family genes of Sanyueli (Prunus salicina Lindl.) and the red-fleshed mutant. Journal of Nuclear Agricultural Sciences, 2020,34(2):247-255. (in Chinese with English abstract) doi: 10.11869/j.issn.100-8551.2020.02.0247

[24]	LI H T, WANG B, ZHANG Q H, et al. Genome-wide analysis of the auxin/indoleacetic acid (Aux/IAA) gene family in allotetraploid rapeseed (Brassica napus L.). BMC Plant Biology, 2017,17:204. DOI:10.1186/s12870-017-1165-5 doi: 10.1186/s12870-017-1165-5

[25]	HAGEN G, GUILFOYLE T. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Molecular Biology, 2002,49(3/4):373-385.

[26]	REED J W. Roles and activities of Aux/IAA proteins in Arabidopsis. Trends in Plant Science, 2001,6(9):420-425. DOI:10.1016/S1360-1385(01)02042-8 doi: 10.1016/S1360-1385(01)02042-8

[27]	MORGAN K E, ZAREMBINSKI T I, THEOLOGIS A. Biochemical characterization of recombinant polypeptides corresponding to the predicted βαα fold in Aux/IAA proteins. Federation of European Biochemical Societies Letters, 1999,454:283-287. DOI:10.1016/s0014-5793(99)00819-4 doi: 10.1016/s0014-5793(99)00819-4

[28]	KORASICK D A, CHATTERJEE S, TONELLI M, et al. Defining a two-pronged structural model for PB1 (Phox/Bem1p) domain interaction in plant auxin responses. Journal of Biological Chemistry, 2015,290(20):12868-12878. DOI:10.1074/jbc.M115.648253 doi: 10

[29]	KEPINSKI S, LEYSER O. The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature, 2005,435(7041):446-451. DOI:10.1038/nature03542 doi: 10.1038/nature03542

[30]	SALEHIN M, BAGCHI R, ESTELLE M. SCFTIR1/AFB-based auxin perception: mechanism and role in plant growth and development. The Plant Cell, 2015,27(1):9-19. DOI:10.1105/tpc.114.133744 doi: 10.1105/tpc.114.133744

[31]	CAO M, CHEN R, LI P, et al. TMK1-mediated auxin signalling regulates differential growth of the apical hook. Nature, 2019,568(7751):240-243. DOI:10.1038/s41586-019-1069-7 doi: 10.1038/s41586-019-1069-7

[32]	DREHER K A, BROWN J, SAW R E, et al. The Arabidopsis Aux/IAA protein family has diversified in degradation and auxin responsiveness. The Plant Cell, 2006,18(3):699-714. DOI:10.1105/tpc.105.039172 doi: 10.1105/tpc.105.039172

[33]	王国平,周东洁,牛永志,等.普通烟草Aux/IAA转录因子家族全基因组鉴定分析.中国烟草学报,2019,25(6):80-89. DOI:10.16472/j.chinatobacco.2019.120 WANG G P, ZHOU D J, NIU Y Z, et al. Genome-wide identification and analysis of Aux/IAA gene family in Nicotiana tobacum. Acta Tabacaria Sinica, 2019,25(6):80-89. (in Chinese with English abstract) doi: 10.16472/j.chinatobacco.2019.120

[34]	蒲全明,施松梅,张林成,等.结球甘蓝AUX/IAA家族基因BoIAA2与BoIAA19的克隆与表达分析.中国农业科技导报,2017,19(9):24-33. DOI:10.13304/j.nykjdb.2016.684 PU Q M, SHI S M, ZHANG L C, et al. Cloning and expression analysis of BoIAA2 and BoIAA19 genes of Aux/IAA family in cabbage. Journal of Agricultural Science and Technology, 2017,19(9):24-33. (in Chinese with English abstract) doi: 10.13304/j.nykjdb.2016.684

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