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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2024, Vol. 50 Issue (2): 280-294    DOI: 10.3785/j.issn.1008-9209.2023.12.221
研究论文     
基因组修饰提高油菜非生物胁迫耐受性及应对气候变化(英文)
黄倩1(),张康妮1,万光龙1,斯燕琼3,周伟军1,2()
1.浙江大学农业与生物技术学院，浙江 杭州 310058
2.农业农村部光谱检测重点实验室，浙江 杭州 310058
3.诸暨市东白缘生态农业科技有限公司，浙江 绍兴 311800
Genome modification improves abiotic stress tolerance in oilseed rape (Brassica napus L.) and responds to climate change
Qian HUANG1(),Muhammad Ahsan FAROOQ1,2(),Kangni ZHANG1,Ahsan AYYAZ1,Guanglong WAN1,Yanqiong SI3,Fakhir HANNAN1,Weijun ZHOU1,2()
1.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
2.Key Laboratory of Spectroscopy Sensing, Ministry of Agriculture and Rural Affairs, Hangzhou 310058, Zhejiang, China
3.Zhuji Dongbaiyuan Ecological Agriculture Technology Co. , Ltd, Shaoxing 311800, Zhejiang, China
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摘要：

当前农业面临多重挑战，其中迫切需要提升产量以满足不断增长的人口需求。盐碱、干旱和极端温度等非生物胁迫是影响植物生长发育和产量的关键因素。油菜作为全球主要的油料作物，是食用植物油、生物柴油和动物饲料的重要来源，研究其遗传变异与生物学特性对于培育能够适应不同环境条件的新品种（基因型）至关重要。本文对近年来国内外油菜抗逆基因的相关研究进展进行了综述，重点总结了新兴分子育种工具和技术，包括全基因组关联分析、基因编辑等在油菜抗逆基因研究中应用的技术，以期为油菜高产抗逆基因的挖掘以及分子育种提供研究基础与技术支撑。
关键词： 油菜;  遗传变异;  非生物胁迫;  株型结构;  基因组改良    
Abstract:

Current agriculture faces the challenge of significantly increasing production to meet the needs of a large human population. Abiotic stresses, such as salinity, drought, and temperature extremes, severely impact plant growth, development and production. Brassica species, particularly Brassica napus L., are important worldwide sources of edible oil, biodiesel and animal feed. Understanding the genetic variation within the Brassica genus is crucial for developing new varieties (genotypes) adapted to different environmental conditions. This paper reviewed the recent advances on stress-resistant genes in B. napus both domestically and internationally, with a particular focus on emerging molecular breeding tools and technologies, including whole-genome association analysis, gene editing and other technologies. This study aimed to contribute to the exploration of high-yielding and stress-resistant genes in B. napus and to provide a research foundation and technical support for molecular breeding.
Key words: Brassica napus L.    genetic variation    abiotic stress    plant architecture    genome improvement
出版日期: 2024-04-30
CLC:  S565  
通讯作者: 周伟军     E-mail: 11616035@zju.edu.cn;wjzhou@zju.edu.cn;ahsanfarooq143@yahoo.com
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黄倩
张康妮
万光龙
斯燕琼
周伟军

引用本文:

黄倩,张康妮,万光龙,斯燕琼,周伟军. 基因组修饰提高油菜非生物胁迫耐受性及应对气候变化(英文)[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(2): 280-294.

Qian HUANG,Muhammad Ahsan FAROOQ,Kangni ZHANG,Ahsan AYYAZ,Guanglong WAN,Yanqiong SI,Fakhir HANNAN,Weijun ZHOU. Genome modification improves abiotic stress tolerance in oilseed rape (Brassica napus L.) and responds to climate change. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(2): 280-294.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.12.221    或    https://www.zjujournals.com/agr/CN/Y2024/V50/I2/280

Study objectiveMarker assisted selectionMethodologyReference
Oleic acid contentSNPsGWAS[7]
Pod number and seed numberSSR, SNPAssociation analysis/QTL mapping[8]
Flowering timeSNPsQTL mapping and transcriptome sequencing[9]
Early senescenceConstruction of B. napus full-length cDNA expression library, plant transformation and selectioniFOX-hunting as a functional genomic tool[10]
Days to flower and seed yieldSSR, AFLPLA[11]
Branch number and branch angleSNP arrayGWAS, LA[12-13]
Number of seeds per siliqueNIL constructMap-based cloning[14]
Seed yield and yield-related traitsSSRQTL analysis and functional gene association in Arabidopsis[15]
Plant architecture and seed yieldBna.AP1.A02 stop codon mutantGene expression analysis[16]
Seed fiber traitsGenetic linkage map 60K SNP arrayQTL analysis[17]
Candidate genes underlying yield-determining traitsSNPs

Genome-wide association and

transcriptome analyses

[18]
Seed oil and seed protein contentsSSR, STS, SRAP, SNPLA[19]
Fatty acid compositionSNPGenome-wide association mapping[20]
Erucic acid content, glucosinolate content, and seed oil contentSNPGWAS[21]
  

Abiotic

stress

Platform/

methodology

Experimental condition/

study objective

CommentReference
SaltQTL mappingSalt toleranceLobed leaf is a common trait, which is related with photosynthesis[67]
GWASPhenotyping of B. napus during seed germination under NaClCandidate genes underlying salt tolerance[74]
Expressed sequence tagSalt and osmotic stressesGrowth and development processes[75]
QTL mappingSalt tolerance and identification of salt-tolerant genesSalt tolerance related gene mapping and cloning[50]
HeatMicroarray analysisHeat stress effects photosynthesis and oil accumulationHeat stress during B. napus seed filling severely impairs yield and oil content[53]
Transcriptomic analysisMechanism of thermosensitive genic male sterility of B. napus under the high temperatureTranscription factors MADS, NFY, HSF, MYB/C and WRKY might play a crucial role in male fertility[76]
GWASHeat stress-tolerance traits in spring-type B. napusA total of five, eight, and seven QTL genes associated with flowering, male sterility, pollen abortion, embryo abortion reducing pollen development[56]
Systematic analysis of Hsf family genes in B. napusDrought and heat stress responsesMultifunctional BnaHsf genes will improve the understanding of plant acclimation response to abiotic stresses[77]
DroughtTranscriptomic basisClassification of the possible drought stress responsive DEGsDEGs in response to drought in B. napus[78]
OverexpressionDrought toleranceTransgenic plants showed a reduced rate of water loss and increased drought tolerance[65]
GWIDrought treatmentVicinal oxygen chelate family in rapeseed (B. napus)[79]
iTRAQLong-term droughtEnergy production, photosynthesis, protein synthesis, stress or defense response, metabolism, signaling, protein folding and degradation[80]

Heavy

metals

Association mappingCd-tolerant QTLsCd-tolerant B. napus that could be utilized in phytore-mediation[67]
High-throughput RNA sequencingIsolation and analysis of putative transporter proteins from B. napusABC genes involved in Cd uptake or transport in B. napus[35]
GWASCd accumulation at the seedling stageCd accumulation and the cloning of candidate Cd accumulation genes, which could be used to help reduce Cd levels in edible plant parts[66]
Genome-wide characterizationAnalysis of metallothinoinein family genesBnaMT3C plays a key role in the response to As3+ stress in B. napus[81]
MicroRNA-mRNAChanges in miRNA and mRNA expression profiles in the roots and shoots of B. napus seedlings under Cd stressCombined miRNA and mRNA profiling revealed miRNAs, genes and pathways involved in Cd response which are potentially critical for adaptation to Cd stress in B. napus[82]
  
  
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