外源5-氨基乙酰丙酸对油菜幼苗硫代葡萄糖苷生物合成的影响(英文)

doi:10.3785/j.issn.1008-9209.2019.08.262

浙江大学学报（农业与生命科学版）

2020, Vol. 46

Issue (3): 291-307 DOI: 10.3785/j.issn.1008-9209.2019.08.262

作物栽培与生理

外源5-氨基乙酰丙酸对油菜幼苗硫代葡萄糖苷生物合成的影响(英文)

MAODZEKA Antony，卢凌志，赵鑫泽，徐颖，吴德志，蒋立希*

浙江大学农业与生物技术学院，浙江省作物种质资源重点实验室，杭州 310058

Effect of exogenous 5-aminolevulinic acid on glucosinolate biosynthesis in rape (Brassica napus L.) seedlings

Antony MAODZEKA(

),Lingzhi LU,Xinze ZHAO,Ying XU,Dezhi WU,Lixi JIANG(

)

Zhejiang Key Laboratory of Crop Gene Resources, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

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摘要：

5-氨基乙酰丙酸(5-aminolevulinic acid, ALA)是一种可以影响植物碳固定和营养物质同化等生理生化过程的植物生长调节剂。本文研究了ALA对幼苗期甘蓝型油菜硫代谢和硫代葡萄糖苷生物合成的影响。用含有0、0.5、1.0、5.0、10.0 mg/L ALA的霍格兰培养液处理油菜幼苗28 d后，分析ALA对其硫醇和硫代葡萄糖苷含量以及相关基因转录组的影响。结果表明：低质量浓度的ALA处理增加了半胱氨酸、硫代葡萄糖苷以及总可溶性硫醇的含量，同时上调了调节硫摄取和代谢相关基因的表达，如BnSULTR1.1、BnSULTR2.2和BnAPK1。由于硫代谢的加速以及甲硫氨酸的积累，ALA还提高了硫代葡萄糖苷的含量，特别是脂肪族硫代葡萄糖苷的含量。从BnUGT74B1和BnUGT74C1基因表达中也可以看出，像脱硫-硫代葡萄糖苷糖基化这样的其他硫代葡萄糖苷的生物合成阶段也因为ALA的作用而加强。高质量浓度的ALA处理，通过造成细胞成分损害的光氧化胁迫而对硫代谢和硫代葡萄糖苷合成产生负调控作用。而中质量浓度的ALA促进了硫的获取和同化，并促进硫代葡萄糖苷的生物合成。

关键词： 5-氨基乙酰丙酸; 硫代谢; 硫代葡萄糖苷; 谷胱甘肽; 油菜幼苗

Abstract:

5-aminolevulinic acid (ALA) has been used as a plant growth regulator and can affect physiochemical processes, including carbon fixation and nutrient assimilation. Here, we investigated the effect of ALA on sulfur metabolism and glucosinolate (GSL) biosynthesis in rape (Brassica napus L.) seedlings, which were treated with 0, 0.5, 1.0, 5.0, and 10.0 mg/L ALA supplemented in a Hoagland solution. After 28 d of treatment, the effect of ALA on thiol and GSL contents and transcriptional profile of associated genes was analyzed. Results showed that low ALA concentrations increased cysteine, GSL, and total soluble thiol contents, and upregulated expression of genes such as BnSULTR1.1, BnSULTR2.2,and BnAPK1 that regulate sulfur uptake and metabolism. ALA also increased the GSL content, particularly the aliphatic GSLs, due to the improvement of sulfur metabolism and assimilation to methionine. Other GSLs biosynthesis stages, such as desulfo-GSL glycosylation, were also significantly improved by the ALA applications as indicated by the increased expression of BnUGT74B1 and BnUGT74C1. High ALA concentrations negatively affected sulfur metabolism and GSL synthesis by inflicting photo-oxidative stress that damaged cellular components. Moderate ALA concentrations promoted sulfur acquisition, assimilation, and GSL biosynthesis.

Key words: 5-aminolevulinic acid sulfur metabolism glucosinolate glutathione rape seedlingCLC number S 565.4 Document code A

出版日期: 2020-06-25

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引用本文:

MAODZEKA Antony, 卢凌志, 赵鑫泽, 徐颖, 吴德志, 蒋立希. 外源5-氨基乙酰丙酸对油菜幼苗硫代葡萄糖苷生物合成的影响(英文)[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(3): 291-307.

Antony MAODZEKA, Lingzhi LU, Xinze ZHAO, Ying XU, Dezhi WU, Lixi JIANG. Effect of exogenous 5-aminolevulinic acid on glucosinolate biosynthesis in rape (Brassica napus L.) seedlings. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(3): 291-307.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.08.262 或 http://www.zjujournals.com/agr/CN/Y2020/V46/I3/291

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