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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2024, Vol. 50 Issue (5): 747-757    DOI: 10.3785/j.issn.1008-9209.2023.07.061
植物保护     
淡紫紫孢菌ZJPL08可湿性粉剂研制及对柑橘木虱的毒力分析
杜丹超(),朱莉,蒲占湑,刘顺民,陈国庆(),鹿连明()
浙江省柑橘研究所，浙江 台州 318020
Development of Purpureocillium lilacinum ZJPL08 wettable powder and its toxicity to Diaphorina citri Kuwayama
Danchao DU(),Li ZHU,Zhanxu PU,Shunmin LIU,Guoqing CHEN(),Lianming LU()
Zhejiang Citrus Research Institute, Taizhou 318020, Zhejiang, China
 全文: PDF(1712 KB)   HTML
摘要：

淡紫紫孢菌（Purpureocillium lilacinum）ZJPL08对柑橘木虱具有强致病性，以该菌为材料的生防菌制剂研发对柑橘木虱的防治具有重要意义。本研究以淡紫紫孢菌ZJPL08为有效成分，进行载体、润湿剂、紫外保护剂等的筛选，确定了淡紫紫孢菌ZJPL08可湿性粉剂的最佳配方：淡紫紫孢菌ZJPL08分生孢子粉10.00%（有效成分）、十二烷基硫酸钠3.00%（润湿剂）、木质素磺酸钠5.00%（分散剂）、腐殖酸0.25%（紫外保护剂）、氯化钙0.10%（孢子萌发促进剂）、黄原胶0.05%（黏着剂）和硅藻土81.60%（载体）。在此条件下制得的淡紫紫孢菌ZJPL08可湿性粉剂孢子含量为3.25×1010 个/g，悬浮率为85.67%，润湿时间为86.22 s，细度为95.39%粉剂过300目筛，pH值为6.65，产品各项指标符合国家农药制剂产品要求。半田间防效试验结果表明，该制剂1 500倍液处理能有效防治柑橘木虱，防效达97.78%，与对照药剂30%噻虫嗪可湿性粉剂1 000倍液（100%防效）相比无显著差异，但显著高于1.80%阿维菌素乳油1 500倍液（78.33%防效）。综上所述，本研究研制的淡紫紫孢菌ZJPL08可湿性粉剂对柑橘木虱具有较好的防效，在替代化学农药防治柑橘木虱上具有良好的应用前景。
关键词： 淡紫紫孢菌;  可湿性粉剂;  柑橘木虱;  生物防治    
Abstract:

The fungus Purpureocillium lilacinum ZJPL08 presents strong pathogenicity against citrus psylla (Diaphorina citri Kuwayama), so the development of a biocontrol agent based on this fungus is of great significance for the prevention and control of D. citri. In this study, P. lilacinum ZJPL08 was used as the active ingredient to screen carriers, wetting agents, and ultraviolet (UV) protectants. The results showed that the optimum formulation for P. lilacinum ZJPL08 wettable powder (WP) was as follows: 10.00% conidial spore powder of ZJPL08 (active ingredient), 3.00% sodium dodecyl sulfate (wetting agent), 5.00% sodium lignosulfonate (dispersant), 0.25% humic acid (UV protectant), 0.10% CaCl2 (promoter of spore germination), 0.05% xanthan gum (adhesive), and 81.60% diatomite (carrier). Under these conditions, the P. lilacinum ZJPL08 WP achieved a spore concentration of 3.25×1010 spores/g, a suspension rate of 85.67%, a wetting time of 86.22 s, a fineness of 95.39% powder passing through a 300 mesh sieve and a pH value of 6.65; all the indexes met the requirements of national pesticide formulation products. The semi-field control efficiency test results showed that the 1 500-fold dilution of P. lilacinum ZJPL08 WP could effectively control D. citri, with a control efficiency of 97.78%, which was not significantly different from the 1 000-fold dilution of the control insecticide, 30% thiamethoxam WP (100% control efficiency), but was significantly greater than the 1 500-fold dilution of 1.80% avemectin emulsion (78.33% control efficiency). In conclusion, the P. lilacinum ZJPL08 WP developed in this study presented excellent control efficiency for D. citri, which probably has important application prospects in replacing chemical pesticides.
Key words: Purpureocillium lilacinum    wettable powder    Diaphorina citri Kuwayama    biocontrol
收稿日期: 2023-07-06 出版日期: 2024-10-31
CLC:  S476.1  
基金资助: 国家重点研发计划项目(2021YFD1400805);浙江省科技计划项目(2023C04030);浙江省基础公益研究计划项目(LGN22C140008)
通讯作者: 陈国庆,鹿连明     E-mail: dcdu84@163.com;cgq5373@163.com;minglu79@126.com
作者简介: 杜丹超（https://orcid.org/0000-0002-4203-6170），E-mail：dcdu84@163.com
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引用本文:

杜丹超,朱莉,蒲占湑,刘顺民,陈国庆,鹿连明. 淡紫紫孢菌ZJPL08可湿性粉剂研制及对柑橘木虱的毒力分析[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(5): 747-757.

Danchao DU,Li ZHU,Zhanxu PU,Shunmin LIU,Guoqing CHEN,Lianming LU. Development of Purpureocillium lilacinum ZJPL08 wettable powder and its toxicity to Diaphorina citri Kuwayama. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(5): 747-757.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.07.061    或    https://www.zjujournals.com/agr/CN/Y2024/V50/I5/747

图1  载体的筛选数据以平均值±标准差表示（n=3）。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图2  润湿剂的筛选数据以平均值±标准差表示（n=3）。不同小写字母表示与对照相比同种润湿剂不同浓度间在P＜0.05水平差异有统计学意义，*表示相同浓度下不同润湿剂间在P＜0.05水平差异有统计学意义。

处理

Treatment

分散效果

Dispersion effect

木质素磺酸钙 CLS＋/－
木质素磺酸钠 SLS＋
蔗糖酯 SE＋
CK5－
表1  分散剂粗筛时的分散效果
图3  分散剂的筛选数据均以平均值±标准差表示（n=3）。不同大写字母表示相同浓度不同分散剂间在P＜0.05水平差异有统计学意义，不同小写字母表示与对照相比同种分散剂不同浓度间在P＜0.05水平差异有统计学意义。
图4  其他助剂的筛选数据以平均值±标准差表示（n=3）。不同大写字母表示相同浓度不同促进剂间在P＜0.05水平差异有统计学意义，不同小写字母表示与对照相比相同处理下不同紫外保护剂、同种促进剂不同浓度间、不同黏着剂间在P＜0.05水平差异有统计学意义。*表示同种紫外保护剂不同处理间在P＜0.05水平差异有统计学意义。

处理

Treatment

防效 Control efficiency/%
3 d7 d14 d

30%噻虫嗪可湿性粉剂

30% thiamethoxam wettable powder

61.67±10.14Ab88.89±5.09Aa100.00±0.00Aa

1.80%阿维菌素乳油

1.80% avemectin emulsion

48.89±11.71ABb60.56±10.84Bab78.33±8.82Ba

菌株ZJPL08可湿性粉剂

ZJPL08 wettable powder

38.89±6.94Bc74.44±5.85ABb97.78±1.92Aa
表2  菌株ZJPL08可湿性粉剂对柑橘木虱的半田间防效
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