牛和小鼠早期胚胎发育过程中细胞谱系发育调控的比较

doi:10.3785/j.issn.1008-9209.2022.12.131

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (6): 765-775 DOI: 10.3785/j.issn.1008-9209.2022.12.131

综述

牛和小鼠早期胚胎发育过程中细胞谱系发育调控的比较

吴潇彤(

),史延,李爽,王少华,张坤(

)

浙江大学动物科学学院，浙江省奶牛遗传改良与乳品质研究重点实验室，浙江杭州 310058

Comparison of cell lineage development and regulation during early embryonic development in cattle and mice

Xiaotong WU(

),Yan SHI,Shuang LI,Shaohua WANG,Kun ZHANG(

)

Key Laboratory of Dairy Cow Genetic Improvement and Milk Quality Research of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China

全文: PDF(2280 KB) HTML

摘要：

高产奶牛繁殖效率低是世界性难题，其中早期胚胎死亡率高是主要原因之一。当前对牛早期胚胎发育的研究有限，而对于小鼠这种模式动物的早期胚胎发育研究已相当深入。因此，本文从胚胎形态、转录因子以及信号通路3方面对牛和小鼠早期胚胎发育过程进行比较，以加深对牛早期胚胎发育的认识。经比较发现，受精后，牛和小鼠早期胚胎在不同时期发生母源因子降解、合子基因组激活、细胞极性建立和不对称分裂，最终使得胚胎形态发生变化，形成具有3个胚层（滋养外胚层、上胚层和原始内胚层）的囊胚。此外，牛和小鼠早期胚胎发育过程中，多个转录因子及信号通路形成复杂网络调控细胞谱系分化。综上所述，在牛和小鼠早期胚胎发育过程中，相似的生物学事件陆续发生，但是细胞谱系分化的调控呈现差异性，提示我们将小鼠早期胚胎作为研究模型的局限性，该领域的研究对提高奶牛繁殖效率以及促进牛遗传改良工作具有重要意义。

关键词： 牛; 小鼠; 着床前; 早期胚胎; 谱系分化; 滋养外胚层; 内细胞团

Abstract:

Low reproductive efficiency of high-yielding dairy cows is a worldwide challenge, among which the high mortality rate of early embryos is one of the main reasons. Scientists have gained insightful knowledge into early embryonic development in mice while very little work has been performed in cattle. To better understand the early embryonic development in cattle, we compared early embryonic development in cattle and mice from the perspectives of embryo morphology, transcription factors and signaling pathways. It was found that after fertilization, degradation of maternal factors, zygotic genome activation, construction of cell polarity and asymmetric division occurred at different periods in the early embryos of cattle and mice. Eventually, embryos develop to blastocysts with three germ layers (trophectoderm, epiblast and primitive endoderm). Furthermore, multiple transcription factors and signaling pathways form complicated networks to regulate cell lineage differentiation during early embryonic development in cattle and mice. In summary, similar biological events occur one after another at the early embryonic development in cattle and mice, but the regulation of cell lineage differentiation is different, suggesting the limitations of using the early embryo of mouse as a research model. The study in this field plays a crucial role in improving reproductive efficiency of dairy cows and promoting the genetic improvement in cattle.

Key words: cattle mouse preimplantation early embryo lineage differentiation trophectoderm inner cell mass

收稿日期: 2022-12-13 出版日期: 2023-12-25

CLC:

Q954.4

基金资助: 浙江省自然科学基金重点项目(LZ21C170001);国家自然科学基金面上项目(32072731);青海省重大科技专项(2021-NK-A5)

通讯作者: 张坤 E-mail: wsxt412726@163.com;kzhang@zju.edu.cn

作者简介: 吴潇彤（https://orcid.org/0000-0002-7669-6695），E-mail：wsxt412726@163.com

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引用本文:

吴潇彤,史延,李爽,王少华,张坤. 牛和小鼠早期胚胎发育过程中细胞谱系发育调控的比较[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(6): 765-775.

Xiaotong WU,Yan SHI,Shuang LI,Shaohua WANG,Kun ZHANG. Comparison of cell lineage development and regulation during early embryonic development in cattle and mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(6): 765-775.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.12.131 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I6/765

图1 小鼠和牛早期胚胎发育过程中的形态变化及关键生物学事件

表1 小鼠和牛早期胚胎发育时期和事件比较

图2 小鼠和牛胚胎第一次细胞谱系分化黑色尖箭头代表促进表达，T形箭头代表抑制表达，带红色“”的箭头代表表达不受影响。

图3 小鼠和牛胚胎第二次细胞谱系分化黑色箭头代表促进表达，带红色“”的箭头代表表达不受影响。

表2 小鼠和牛早期胚胎发育生物学机制比较

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