FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响

doi:10.3785/j.issn.1008-9209.2021.12.061

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (2): 261-268 DOI: 10.3785/j.issn.1008-9209.2021.12.061

动物科学与动物医学

FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响

刘辰晖¹(

),吴海龙²(

),程蕾¹(

),余婕¹,夏瑜¹,向敏¹,胡修忠¹,王定发¹,陶弼菲¹

^1.武汉市农业科学院，湖北武汉 430065
^2.武汉光明生态示范场奶牛有限公司，湖北武汉 430045

Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China

Chenhui LIU¹(

),Hailong WU²(

),Lei CHENG¹(

),Jie YU¹,Yu XIA¹,Min XIANG¹,Xiuzhong HU¹,Dingfa WANG¹,Bifei TAO¹

^1.Wuhan Academy of Agricultural Sciences, Wuhan 430065, Hubei, China
^2.Wuhan Bright Dairy Ecological Demonstration Dairy Farm Co. , Ltd. , Wuhan 430045, Hubei, China

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摘要：

为探究FYN结合蛋白（FYN binding protein,FYB）基因突变对中国南方荷斯坦牛泌乳性能的影响，以785头中国南方地区荷斯坦牛为研究对象，收集其2014—2020年的泌乳性状数据，通过SNaPshot技术对FYB基因突变位点进行单核苷酸多态性检测，分析突变对FYB蛋白结构和理化性质的影响，并利用最小二乘法将突变与奶牛泌乳性状、血清生化指标进行关联分析。结果显示：在试验群体中，FYB基因rs109262355位点等位基因C和T的频率分别为0.36和0.64，符合哈代-温伯格（Hardy-Weinberg）平衡（P＞0.05）；该位点由C→T的突变会导致FYB蛋白对应区域形成1个额外的长度为3.34 ?的氢键，同时使该区域的蛋白疏水性和柔性降低；该位点CC和TT基因型个体的305 d产奶量、高峰产奶量及乳脂率均存在显著差异（P＜0.05），等位基因T为产奶量优势等位基因，等位基因C为乳脂率优势等位基因；该位点CC和TT基因型个体血清中低密度脂蛋白（low-density lipoprotein, LDL）含量存在显著差异（P＜0.05），碱性磷酸酶（alkaline phosphatase, ALP）活性和总胆固醇（total cholesterol, TC）含量存在极显著差异（P＜0.01）。FYB基因rs109262355突变能够影响奶牛产奶量及乳脂率，可作为候选遗传标记应用于中国南方荷斯坦牛泌乳性状的标记辅助选择育种。

关键词： 荷斯坦牛; FYN结合蛋白基因; 泌乳性能; 等位基因

Abstract:

This study aimed to investigate the effects of FYN binding protein (FYB) gene mutation on lactation performance of Holstein cows in southern China. The lactation traits of 785 Holstein cows from 2014 to 2020 were collected, and the single nucleotide polymorphisms (SNPs) in FYB gene were detected by SNaPshot and the effects of the mutation on structural and physicochemical properties of FYB protein were analyzed. The associations between the mutations and lactation traits and serum biochemical indexes were analyzed by the least square method. The results showed that the allele frequencies of C and T at rs109262355 in FYB gene were 0.36 and 0.64, respectively, which were consistent with Hardy-Weinberg equilibrium (P＞0.05). An extra hydrogen bond with a length of 3.34 ? in the related region of FYB protein caused by the mutation from C to T at rs109262355 was formed, which reduced the hydrophobicity and flexibility of the protein in this region. 305 d milk yield, peak milk yield, and milk fat rate between CC and TT genotype individuals in rs109262355 was significantly different (P＜0.05), with the dominant allele T of milk yield and the dominant allele C of milk fat rate. The serum low-density lipoprotein (LDL) was significantly different between CC and TT genotype individuals (P＜0.05); alkaline phosphatase (ALP) and total cholesterol (TC) in serum were highly significantly different between CC and TT genotype individuals (P＜0.01). The rs109262355 mutation in FYB gene can affect the milk yield and milk fat percentage of dairy cows, which could be used as a candidate genetic marker in lactation traits of Holstein cows in southern China of marker-assisted selection breeding.

Key words: Holstein cows FYN binding protein gene lactation performance allele

收稿日期: 2021-12-06 出版日期: 2023-04-25

CLC:

S823.91

基金资助: 武汉市农业科学院创新项目(CXJSFW202002-3);湖北省自然科学基金项目(2021CFB531)

通讯作者: 程蕾 E-mail: lchhhcl890621@sina.com;wuhailong1004@126.com;chenglei_011@126.com

作者简介: 刘辰晖（https://orcid.org/0000-0003-0942-4662），E-mail：lchhhcl890621@sina.com|吴海龙（https://orcid.org/0000-0003-4019-608X），E-mail：wuhailong1004@126.com

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引用本文:

刘辰晖,吴海龙,程蕾,余婕,夏瑜,向敏,胡修忠,王定发,陶弼菲. FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(2): 261-268.

Chenhui LIU,Hailong WU,Lei CHENG,Jie YU,Yu XIA,Min XIANG,Xiuzhong HU,Dingfa WANG,Bifei TAO. Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 261-268.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.12.061 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I2/261

表1 FYB基因rs109262355突变详细信息

表2 rs109262355突变在试验群体中的分布情况

图1 rs109262355突变对FYB蛋白结构和理化性质的影响A. FYB蛋白保守域和三维结构模型（rs109262355位点对应氨基酸所在位置用橙色标注，PHA03247超家族、SH3超家族和hSH3蛋白保守域分别用蓝色、绿色和红色标注）；B～C. rs109262355位点核苷酸分别为C和T时189—289位氨基酸所在区域的三维结构模型；D～E. rs109262355位点核苷酸从C（FYB-O）到T（FYB-M）的突变分别对FYB蛋白疏水性和柔性的影响。

表3 rs109262355突变对试验群体泌乳性状的影响

表4 rs109262355突变对试验群体血清生化指标的影响

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