转录因子OsbHLH59通过调控木聚糖酶抑制蛋白OsXIP表达水平影响水稻抗褐飞虱的机制研究（英文）

doi:10.3785/j.issn.1008-9209.2021.07.072

浙江大学学报（农业与生命科学版）

2022, Vol. 48

Issue (4): 453-464 DOI: 10.3785/j.issn.1008-9209.2021.07.072

植物保护

转录因子OsbHLH59通过调控木聚糖酶抑制蛋白OsXIP表达水平影响水稻抗褐飞虱的机制研究（英文）

吕雪祺，许颖，黄莹莹，刘明启，翁晓燕

^1.浙江大学生命科学学院植物生物学研究所，杭州 310058
^2.中国计量大学生命科学学院，杭州 310018

OsbHLH59 involved in rice resistance to Nilaparvata lugens(St?l) by regulating the expression level of xylanase inhibitor protein OsXIP

Xueqi Lü¹(

),Ying XU¹,Yingying HUANG¹,Mingqi LIU²,Xiaoyan WENG¹(

)

^1.Institute of Plant Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
^2.College of Life Sciences, China Jiliang University, Hangzhou 310018, China

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摘要：

木聚糖酶抑制蛋白（xylanase inhibitor protein, XIP）在植物防御中发挥作用，实验室前期研究发现，水稻木聚糖酶抑制蛋白OsXIP能响应褐飞虱［Nilaparvata lugens (St?l)］胁迫。转录因子OsbHLH59可以与OsXIP的启动子结合以响应褐飞虱侵害。本研究利用转基因技术获得OsbHLH59过表达水稻株系并利用CRISPR/Cas9系统获得了OsbHLH59突变水稻株系，研究了转录因子OsbHLH59对OsXIP表达水平的影响及其在水稻抗褐飞虱中的作用。结果表明，OsbHLH59的过表达引起了OsXIP表达水平的上调。褐飞虱侵害对OsbHLH59过表达株系和OsXIP基因表达水平高的株系的生长水平影响相较野生型更小。与野生型相比，过表达OsbHLH59和OsXIP基因均降低了褐飞虱的取食偏好。同时，OsbHLH59过表达株系和OsXIP过表达株系的防御相关基因表达水平更高，H₂O₂含量更低，保护性酶活性也更高。综上所述，OsbHLH59可以提高OsXIP的表达，OsXIP在水稻响应褐飞虱胁迫中发挥了重要作用。

关键词： 木聚糖酶抑制蛋白; OsXIP; OsbHLH59; 基因表达; 植物防御

Abstract:

Xylanase inhibitor protein (XIP) is considered to participate in plant defense. OsXIP was found to be a stress-responsive gene of Nilaparvata lugens (St?l) in the previous study. The transcription factor OsbHLH59 could be combined with the promoter of OsXIP in response to brown planthopper (BPH) treatment. In order to study whether OsbHLH59 was involved in regulating the expression of OsXIP and mediating rice resistance to BPH, OsbHLH59 overexpression transgenic lines were obtained by transgenic methods, and OsbHLH59 mutant lineswere obtained by using CRISPR/Cas9 system. The results showed that the expression levels of xylanase inhibitor OsXIP enhanced accompanied with the increased overexpression level of transcription factor gene OsbHLH59. Through the determination of agronomic traits, it was found that the growth of OsbHLH59 overexpression lines and OsXIP overexpression lines was less affected by the BPH treatment compared with wild type (WT). When WT plants and transgenic plants were exposed to BPH, the feeding preference of BPH in OsbHLH59 overexpression and OsXIP overexpression lines reduced compared with that of WT. With the BPH treatment, higher expression levels of defense-related genes were found in OsbHLH59 overexpression and OsXIP overexpression lines. In addition, compared with WT, OsbHLH59 overexpression and OsXIP overexpression lines had stronger abilities to remove excess H₂O₂ and higher antioxidant enzyme activities. This study reveals that OsbHLH59 can activate the expression of OsXIP, and OsXIP participates in rice resistance to BPH.

Key words: xylanase inhibitor protein OsXIP OsbHLH59 gene expression plant defence

出版日期: 2022-08-25

CLC:

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引用本文:

吕雪祺, 许颖, 黄莹莹, 刘明启, 翁晓燕. 转录因子OsbHLH59通过调控木聚糖酶抑制蛋白OsXIP表达水平影响水稻抗褐飞虱的机制研究（英文）[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(4): 453-464.

Xueqi Lü, Ying XU, Yingying HUANG, Mingqi LIU, Xiaoyan WENG. OsbHLH59 involved in rice resistance to Nilaparvata lugens(St?l) by regulating the expression level of xylanase inhibitor protein OsXIP. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(4): 453-464.

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https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.07.072 或 https://www.zjujournals.com/agr/CN/Y2022/V48/I4/453

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