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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (5): 596-602    DOI: 10.3785/j.issn.1008-9209.2018.10.252
动物科学与动物医学     
精胺对鹅免疫器官指数及免疫相关因子基因表达的影响
易治鑫1,2(),蒋易龙1,王秋宏1,徐麒麟3,王新兴1,莫桂林1,姜冬梅1,康波1()
1. 四川农业大学动物科技学院，成都 611130
2. 巴中市农林科学研究院，四川 巴中 636000
3. 成都市农林科学院，成都 611130
Effects of spermine on immune organ indexes and expression levels of genes related to immune factors in geese
Zhixin YI1,2(),Yilong JIANG1,Qiuhong WANG1,Qilin XU3,Xinxing WANG1,Guilin MO1,Dongmei JIANG1,Bo KANG1()
1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
2. Bazhong Academy of Agriculture and Forestry Sciences, Bazhong 636000, Sichuan, China
3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China
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摘要：

为研究精胺对鹅免疫器官指数及免疫相关因子基因表达的影响，用5和10 mg/kg精胺灌喂成年四川白鹅雌鹅，测定其肝指数、胸腺指数、脾指数和法氏囊指数，检测在各免疫器官组织中TNF-α、IL-1β、IL-6和IFN-γ基因表达量和多胺含量；对照组灌喂禽用生理盐水。结果表明：10 mg/kg精胺灌喂组的鹅肝指数显著低于对照组（P＜0.05）；5 mg/kg精胺灌喂组的鹅胸腺和法氏囊中TNF-α、IL-1β、IL-6和IFN-γ表达量均显著低于对照组（P＜0.05）；10 mg/kg精胺灌喂组的鹅法氏囊中TNF-α和IFN-γ及肝中IFN-γ表达量均显著低于对照组（P＜0.05）；外源性精胺显著下调脾中IFN-γ表达量（P＜0.05）；精胺灌喂组的鹅肝内精胺含量显著高于对照组（P＜0.05）。说明精胺可通过介导鹅免疫相关因子基因表达来参与调控鹅免疫功能，并且存在剂量依赖性和组织特异性。
关键词： 精胺;  鹅;  免疫器官指数;  免疫因子;  基因表达    
Abstract:

To investigate the effects of spermine on goose immune organ indexes and expression levels of genes related to immune factors, the Sichuan adult white geese (female) were intragastrically administered with 5 and 10 mg spermine per kilogram body mass, and then the indexes of liver, thymus, spleen and bursa of Fabricius were measured. In addition, the expression levels of TNF-α, IL-1β, IL-6 and IFN-γ genes and polyamine contents in the liver, thymus, spleen and bursa of Fabricius were determined. The control group was intragastrically administered with physiological saline. The results showed that the liver index in the geese treated with 10 mg/kg spermine was significantly lower than the control group (P＜0.05). The expression levels of TNF-α, IL-1β, IL-6 and IFN-γ genes in the thymus and bursa of Fabricius of geese treated with 5 mg/kg spermine were significantly lower than the control group (P＜0.05), respectively. The expression levels of TNF-α and IFN-γ in the bursa of Fabricius and IFN-γ in the liver of geese treated with 10 mg/kg spermine were significantly lower than the control group (P＜0.05), respectively. The expression level of IFN-γ was significantly decreased in the spleen by the treatments of spermine compared with the control group (P＜0.05). The contents of spermine in the liver of geese treated with spermine were significantly greater than the control group (P＜0.05). In summary, these results suggest that spermine regulates the immunologic function of geese by mediating the expression levels of genes related to immune factors with the tissue-specific and dose-dependent manner.
Key words: spermine    goose    immune organ index    immune factor    gene expression
收稿日期: 2018-10-25 出版日期: 2019-12-05
CLC:  S 835  
基金资助: 国家自然科学基金(31872358);四川省教育厅青年基金(18ZB0469)
通讯作者: 康波     E-mail: yizhixin926@163.com;bokang@sicau.edu.cn
作者简介: 易治鑫（https://orcid.org/0000-0002-7895-7836），E-mail：yizhixin926@163.com
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易治鑫
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徐麒麟
王新兴
莫桂林
姜冬梅
康波

引用本文:

易治鑫,蒋易龙,王秋宏,徐麒麟,王新兴,莫桂林,姜冬梅,康波. 精胺对鹅免疫器官指数及免疫相关因子基因表达的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(5): 596-602.

Zhixin YI,Yilong JIANG,Qiuhong WANG,Qilin XU,Xinxing WANG,Guilin MO,Dongmei JIANG,Bo KANG. Effects of spermine on immune organ indexes and expression levels of genes related to immune factors in geese. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(5): 596-602.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.10.252    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I5/596

基因

Genes

引物序列（5′→3′）

Primer sequences (5′→3′)

产物长度

Product size/bp

退火温度

Annealing

temperature/℃

登录号

Accession

number

扩增效率

Amplification

efficiency/%

TNF-αF:GAATGAACCCTCCTCCG13960.0EU37529698.7
R:ATCTGGTAACAGGAAGG
IL-1βF:CCGCAAAGTGAGGCTCAACA10055.0JF505290.199.1
R:GTAGCCTTTGATGCCCAGC
IL-6F:AGATGGTGATAAATCCTGATGA15060.0XM_013171777.199.3
R:CGGTTTTCTCCATAAATGAAGT
IFN-γF:TGAGCCAGATTGTTTCCC14663.3XM_013198313.1100.5
R:CAGGTCCACGAGGTCTTT
GAPDHF:GTGGTGCAAGAGGCATTGCTGAC8665.0MG674174.199.8
R:GCTGATGCTCCCATGTTCGTGAT
表1  引物序列信息
图1  鹅免疫器官组织总RNA电泳图1：肝；2：胸腺；3：脾；4：法氏囊。
图2  鹅肝组织目的基因RT-PCR产物电泳图M：DL2000标志物。
图3  目的基因实时荧光定量PCR熔解曲线
图4  精胺对鹅免疫器官指数的影响短栅上不同小写字母表示不同处理间在P＜0.05水平差异有统计学意义，未标字母者表示在P＜0.05水平差异无统计学意义；n=6。
图5  精胺对鹅免疫相关因子基因表达的影响短栅上不同小写字母表示不同处理间在P＜0.05水平差异有统计学意义，未标字母者表示在P＜0.05水平差异无统计学意义；n=6。
图6  精胺对鹅免疫器官组织多胺水平的影响短栅上不同小写字母表示不同处理间在P＜0.05水平差异有统计学意义，未标字母者表示在P＜0.05水平差异无统计学意义；n=6。
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