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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (6): 631-641    DOI: 10.3785/j.issn.1008-9209.2011.06.007
生物科学与技术     
叶片喷施C1 化合物对拟南芥生理特性和C1 代谢、光合作用及胁迫相关基因表达的影响
王莎莎, 孙利利, 肖素勤, 周磊, 孙振, 陈丽梅
昆明理工大学生命科学与技术学院生物工程技术研究中心,云南呈贡６５０５００
Effects of foliar spraying C1 compounds on physiological characters and expression levels of genes related to C1 metabolism , photosynthesis and stress response of Arabidopsis thaliana
WANG Sha‐sha , SUN Li‐li , XIAO Su‐qin , ZHOU Lei , SUN Zhen , CHEN Li‐mei
Biology Engineering and Technology Research Centre , Institute of l i f e Science and Technology , Kunming University of Science and Technology , Chenggong , Yunnan 650500 , China
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摘要： 为了比较甲醇、甲醛和甲酸３ 种C１化合物对植物生理特性及基因表达的影响,更好地理解这３种C１化合物的作用机制,分别用５ mmol.L － １ 甲醇、甲醛、甲酸溶液均匀喷洒于温室条件下盆栽培养的拟南芥叶片上,以喷蒸馏水的植物为对照,每周喷１次,４周后分析施用这３种C１ 化合物对其生长及生理特性的影响.结果表明:甲醇促进拟南芥生长,而甲醛和甲酸抑制其生长,甲醛的抑制作用特别明显;甲醇处理显著降低了叶片花青素含量但提高了叶绿素a 含量及叶绿素a/b 的比值,甲醛处理显著降低了叶绿素a 含量及叶绿素a/b 的比值,甲酸处理显著提高了叶绿素a 含量但大幅度降低了叶绿素b 含量从而使叶绿素a/b 的比值比对照高２ 倍;３种C１化合物处理都能增加叶片中可溶性总蛋白质含量,甲醇处理尤为明显,只有甲醛处理可显著提高叶片可溶性总糖、H２O２ 及羰基化蛋白质含量,产生氧化胁迫.对基因表达谱进行逆转录聚合酶链式反应(RT‐PCR)的结果表明:所选的大多数光合作用相关基因的表达被甲醇诱导,而甲醛抑制它们的表达但诱导很多胁迫相关基因的表达,甲酸对大多数光合作用相关基因的表达没有显著影响;３种C１化合物对大多数C１代谢相关基因没有显著影响,但都抑制５ ,１０‐亚甲基‐四氢叶酸还原酶基因的表达.
Abstract: In order to compare the effects of methanol , formaldehyde and formic acid application on plant physiological characters and gene expressions and to better understand the functional mechanisms of the three C１ compounds , ５ mmol.L － １ methanol , formaldehyde and formic acid were sprayed , respectively , on the leaves of A rabidopsis grown under greenhouse conditions once a week , using the plants sprayed with distilled water as the control . After four weeks , the effects of foliar spraying these C １ compoundson plant growth and physiological characters were analyzed . The results indicated that methanol stimulated the growth of A rabidopsis , whereas formaldehyde and formic acid inhibited its growth and the nhibition of formaldehyde was much remarkable . Application of methanol decreased anthocyanin content but increased chlorophyll ( Chl) a content and a/b ratio significantly . On the contrary , formaldehyde reatment reduced Chl a content and a/b ratio markedly . Application of formic acid caused an obvious increase in Chl a content and a notable decrease in Chl b content , which led to ２‐fold increase in Chl a/b ratio . Application of three C１ compounds all increased total leaf soluble protein content ,and the effect of methanol spraying was especially remarkable . Only formaldehyde treatment elevated the levels of soluble
sugars , H２ O２ and carbonyl‐proteins in leaves , suggesting occurrence of oxidative stress . Gene expression profiles by reverse transcription‐polymerase chain reaction (RT‐PCR) showed that methanol stimulation induced expressions of most selected photosynthesis‐related genes , while formaldehyde repressed their expressions but up‐regulated most of the selected stress‐responsive genes . The expressions of most selected photosynthesis‐related genes were not affected significantly by the application of formic acid . Interestingly , three C１ compounds had no remarkable impacts on the expressions of most selected genes involved in C１ metabolism , but all repressed the expression of ５ ,１０‐methylene‐tetrahydrofolate ( THF) reductase .
出版日期: 2011-11-25
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王莎莎
孙利利
肖素勤
周磊
孙振
陈丽梅

引用本文:

王莎莎, 孙利利, 肖素勤, 周磊, 孙振, 陈丽梅. 叶片喷施C1 化合物对拟南芥生理特性和C1 代谢、光合作用及胁迫相关基因表达的影响[J]. 浙江大学学报(农业与生命科学版), 2011, 37(6): 631-641.

WANG Sha‐sha,SUN Li‐li,XIAO Su‐qin,ZHOU Lei,SUN Zhen,CHEN Li‐mei. Effects of foliar spraying C1 compounds on physiological characters and expression levels of genes related to C1 metabolism , photosynthesis and stress response of Arabidopsis thaliana. , 2011, 37(6): 631-641.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.06.007    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I6/631

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