鹅NLRC5序列特征及组织表达谱分析

doi:10.3785/j.issn.1008-9209.2021.03.012

浙江大学学报（农业与生命科学版）

2022, Vol. 48

Issue (2): 254-260 DOI: 10.3785/j.issn.1008-9209.2021.03.012

动物科学与动物医学

鹅NLRC5序列特征及组织表达谱分析

赵帅¹(

),顾天天¹,侯丽娥¹,张扬¹,曹正锋¹,陈国宏^1,²,徐琪¹,张钰¹(

)

^1.扬州大学动物科学与技术学院，江苏扬州 225009
^2.扬州大学教育部农业与农产品安全国际合作联合实验室，江苏扬州 225009

NLRC5 sequence characteristics and analysis of tissue expression profile in goose (Anser cygnoides)

Shuai ZHAO¹(

),Tiantian GU¹,Li’e HOU¹,Yang ZHANG¹,Zhengfeng CAO¹,Guohong CHEN^1,²,Qi XU¹,Yu ZHANG¹(

)

^1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
^2.Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China

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摘要：

作为NOD样受体（NOD-like receptor, NLR）家族重要成员之一的NLRC5，在机体识别入侵微生物、传递免疫信号及调节先天性免疫反应中具有重要作用。本研究以扬州鹅为研究对象，分析鹅NLRC5序列特征，并通过实时荧光定量聚合酶链反应（real-time fluorescent quantitative polymerase chain reaction, RT-qPCR）检测NLRC5基因的组织表达谱，同时检测雏鹅感染肠炎沙门菌0、1、2、4 d后肝脏、脾脏和肾脏中NLRC5的表达水平。结果显示：鹅NLRC5氨基酸序列在脊椎动物中高度保守，其中鹅与鸭NLRC5序列同源性为89.12%；NLRC5基因在心脏、肝脏、脾脏、肺、肾脏、十二指肠、肌胃、大脑和肌肉等组织中均有表达，其中在脾脏中的表达水平最高；此外，雏鹅感染肠炎沙门菌后其肝脏、脾脏和肾脏中NLRC5的表达量在1～2 d内显著上升（P＜0.05），然后在感染4 d内恢复至正常水平。上述研究结果有助于理解NLRC5在鹅感染肠炎沙门菌免疫应答中的作用。

关键词： NLRC5; 鹅; 先天性免疫反应; 肠炎沙门菌

Abstract:

As one of the important members of the NOD-like receptor (NLR) family, NLRC5 plays an important role in the recognition of invading microorganisms, immune signal transmission and innate immune response regulation in vivo, but there are relatively few researches on NLRC5 in poultry. In this study, the Yangzhou goose was selected, and the sequence characteristics of NLRC5 was analyzed, and the expression profile of NLRC5 gene was detected by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). The expression levels of NLRC5 in the liver, spleen and kidney of goslings infected with Salmonella enteritidis at 0, 1, 2 and 4 d post-infection were examined. The results showed that the amino acid sequence of goose NLRC5 was highly conserved in vertebrates, and shared high homology with duck (89.12%). The NLRC5 mRNA was widely expressed in the heart, liver, spleen, lung, kidney, duodenum, gizzard, brain and muscle, and its expression level was the highest in the spleen. In addition, the expressions of NLRC5 in the liver, spleen and kidney of goslings infected with S.enteritidis were significantly upregulated during 1-2 d, and then returned to normal at 4 d post-infection. The above results are helpful to understand the role of NLRC5 in the immune response of goose infected with S. enteritidis.

Key words: NLRC5 goose innate immune response Salmonella enteritidis

收稿日期: 2021-03-01 出版日期: 2022-04-29

CLC:

S 835

基金资助: 国家现代农业产业技术体系(CARS-42-3);江苏省扬州市现代农业重点研发计划(YZ2020053)

通讯作者: 张钰 E-mail: 626981977@qq.com;yuzhang@yzu.edu.cn

作者简介: 赵帅（https://orcid.org/0000-0002-2188-8585），E-mail：626981977@qq.com

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引用本文:

赵帅,顾天天,侯丽娥,张扬,曹正锋,陈国宏,徐琪,张钰. 鹅NLRC5序列特征及组织表达谱分析[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(2): 254-260.

Shuai ZHAO,Tiantian GU,Li’e HOU,Yang ZHANG,Zhengfeng CAO,Guohong CHEN,Qi XU,Yu ZHANG. NLRC5 sequence characteristics and analysis of tissue expression profile in goose (Anser cygnoides). Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(2): 254-260.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.03.012 或 https://www.zjujournals.com/agr/CN/Y2022/V48/I2/254

表1 RT-qPCR引物信息

图1 NLRC5氨基酸序列分析

表2 各物种的NLRC5氨基酸序列同源性比对 (%)

图2 NLRC5 基因的进化树分析

图3 鹅 NLRC5 基因在各组织中的表达谱分析短栅上不同小写字母表示在P＜0.05水平差异有统计学意义，下同。

图4 肠炎沙门菌感染后雏鹅不同组织中 NLRC5 mRNA的表达量变化A.肝脏；B.脾脏；C.肾脏。

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