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| Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties |
| Ling-hui Zeng, Hua-dan Zhang, Cai-ju Xu, Yu-jia Bian, Xue-jiao Xu, Qiang-min Xie, Rong-hua Zhang |
| Department of Pharmacy, Zhejiang University City College, Hangzhou 310015, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China; Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China |
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Abstract A relationship between status epilepticus (SE) and oxidative stress has recently begun to be recognized. To explore whether the flavonoids extracted from licorice (LFs) have any protective effect on kainate (KA)-induced seizure in mice, we treated mice with LFs before and after KA injection. In KA-treated mice, we found that superoxide dismutase (SOD) activity decreased immediately after the onset of seizure at 1 h and then increased at 6 h. It returned to baseline 1 d after seizure and then increased again at 3, 7, and 28 d, while malondialdehyde (MDA) content remained at a high level at 1 h, 6 h, 3 d, 7 d, and 28 d, indicating a more oxidized status related to the presence of more reactive oxygen species (ROS). Treatment with LFs before KA injection reversed the seizure-induced change in SOD activity and MDA content at 1 h, 6 h, 3 d, 7 d, and 28 d. Treatment with LFs after seizure decreased KA-induced SOD activity and MDA content at 7 and 28 d. Also, LF pre- and post-KA treatments decreased seizure-induced neuronal cell death. Subsequently, Morris water maze tests revealed that the escape latency was significantly decreased and the number of target quadrant crossings was markedly increased in the LF-treated groups. Thus, our data indicate that LFs have protective effects on seizure-induced neuronal cell death and cognitive impairment through their anti-oxidative effects.
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Received: 13 May 2013
Published: 04 November 2013
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