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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (7): 580-585    DOI: 10.1631/jzus.B1400234
Articles     
Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro
Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang
Department of Hematology, the First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310006, China; Department of Hematology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China
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Abstract  To investigate the potential effects of pure total flavonoid compounds (PTFCs) from Citrus paradisi Macfadyen separately or combined with arsenic trioxide on the proliferation of human myeloid leukemia cells and the mechanisms underlying the action of PTFCs. The effects of PTFCs separately or combined with arsenic trioxide on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fluorescence microscopy, and flow cytometry. Their effects on the expression levels of apoptosis-related regulators were determined by Western blot assay. PTFCs combined with arsenic trioxide significantly inhibited the growth of Kasumi-1 cells, and apoptosis was confirmed by flow cytometry analysis. Hoechst 33258 staining showed more significant morphological changes and more apoptosis following the combined treatment. Western blots showed changes in the expression of genes for poly ADP-ribose polymerase (PARP), caspase 3/9, and P65. The results indicated that PTFCs separately or combined with arsenic trioxide inhibited proliferation of leukemia cells in vitro and induced their apoptosis by modulating the expression of apoptosis-related regulator genes.

Key words： Pure total flavonoid compounds      Human myeloid leukemia cells      Growth inhibition      Synergistic effect      Apoptosis     
Received: 26 August 2014      Published: 03 July 2015
CLC:  R733.7  
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Bo Wang
Jian-ping Jiang
Sheng-yun Lin
Ying-ying Shen
Li-qiang Wu
Zhen-zhen Chen
Jing Li
Zhi Chen
Wen-bin Qian
Cite this article:

Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang. Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(7): 580-585.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400234     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I7/580

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