SA/PEO composite scaffold electrohydrodynamic direct writing fabrication and influencing factors analysis

doi:10.3785/j.issn.1008-973X.2025.07.021

Journal of ZheJiang University (Engineering Science)

2025, Vol. 59

Issue (7): 1532-1538 DOI: 10.3785/j.issn.1008-973X.2025.07.021

SA/PEO composite scaffold electrohydrodynamic direct writing fabrication and influencing factors analysis

Lei SUN1(

),Chunjing WANG1,Wenlei ZHANG1,Zhipeng MA2,Yongqiang CHENG1

1. College of Integrated Circuits, Taiyuan University of Technology, Taiyuan 030024, China
2. School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310058, China

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Abstract

To improve the printing accuracy of electrohydrodynamic direct writing (EHDDW) in tissue engineering scaffold preparation, and to reduce equipment production costs, a low-cost EHDDW printing platform was constructed independently. Sodium alginate (SA)/polyethylene oxide (PEO) composite bioinks with varying concentration gradients were developed, and the biological characterization and printing test results reveal that the 2SA/7PEO composite bioink exhibits good cytocompatibility and EHDDW printing performance. Through theoretical analysis and experimental study, the influence mechanism of printing parameters on the fiber width of composite bioink was explored. Results show that increasing the voltage, decreasing the fluid flow rate, and accelerating the substrate movement speed can enhance the resolution of fibers. The SA/PEO composite bioink achieved a maximum printing resolution of 2.48±0.27 μm with an applied voltage of 2 400 V, a syringe pump flow rate of 200 nL/min, and a substrate movement speed of 300 mm/min. Single-layer/multi-layer grid micropatterns were prepared using fine-jet mode/cone-jet mode, proving that the self-constructed EHDDW printing platform has the potential to prepare complex and multilayered bio-microstructures.

Key words： electrohydrodynamic direct writing (EHDDW) sodium alginate (SA) polyethylene oxide (PEO) cone-jet mode tissue engineering

Received: 14 June 2024 Published: 25 July 2025

CLC:

TH 16

Fund: 山西省自然科学基金资助项目（202103021223069）.

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	Lei SUN
	Chunjing WANG
	Wenlei ZHANG
	Zhipeng MA
	Yongqiang CHENG

Cite this article:

Lei SUN,Chunjing WANG,Wenlei ZHANG,Zhipeng MA,Yongqiang CHENG. SA/PEO composite scaffold electrohydrodynamic direct writing fabrication and influencing factors analysis. Journal of ZheJiang University (Engineering Science), 2025, 59(7): 1532-1538.

URL:

https://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2025.07.021 OR https://www.zjujournals.com/eng/Y2025/V59/I7/1532

海藻酸钠/聚氧化乙烯支架电直写成型及影响因素分析

为了提升电流体动力直写（EHDDW）在组织工程支架制备中的打印精度，降低设备及生产成本，自主搭建低成本电直写打印平台. 开发不同浓度梯度的海藻酸钠（SA）/聚氧化乙烯（PEO）复合生物墨水，生物学表征及打印测试结果表明，2SA/7PEO复合生物墨水具有良好的细胞相容性及电直写性能. 结合理论分析及实验探究打印参数对复合生物墨水沉积纤维宽度的影响机制，结果表明，增大电压、减小供液流率及提升基板移速均可提高纤维分辨率. 当电压为2 400 V，注射泵流率为200 nL/min，基板移速为300 mm/min时，SA/PEO复合生物墨水的最高打印精度为2.48±0.27 μm. 采用微射流模式/锥射流模式制备SA/PEO复合生物墨水单层/多层网格微图案，证明了自主搭建的电直写打印平台具备制备复杂、多层次生物微结构的潜力.

关键词： 电流体动力直写(EHDDW), 海藻酸钠(SA), 聚氧化乙烯(PEO), 锥射流模式, 组织工程

Fig.1 Printing platform of electrohydrodynamic direct writing

Fig.2 Preparation process of sodium alginate/polyethylene oxide hydrogels

Fig.3 Biological characterization of sodium alginate/polyethylene oxide hydrogels

Fig.4 Electrohydrodynamic direct writing printing sodium alginate/polyethylene oxide composite bioinks

Tab.1 Turn-on voltage of composite bioink in two jetting modes

Fig.5 Different modes of 2SA/7PEO composite bioinks

Fig.6 Influence of voltage on linewidth of deposited fibers

Fig.7 Influence of syringe pump flow rate on linewidth of deposited fibers

Fig.8 Influence of substrate movement speed on linewidth of deposited fibers

Fig.9 Grid structure of different jetting modes


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