A novel joint was proposed for connecting hybrid load beams and concrete-filled steel tube columns for long-span prefabricated structures. Cyclic reciprocating tests were conducted on two full-scale interior joint specimens to investigate the seismic performance and mechanical performance of the proposed joint. Two joint specimens were designed with different types of hybrid steel-concrete beams, i.e., reduced beam section (RBS) and untreated H-steels were respectively used at the beam ends. The seismic performance of the joints was analyzed comprehensively based on the failure pattern, dissipated energy, bearing capacity, ductility and strain distribution along the precast hybrid beams. Test results showed that the specimen with RBS region can promote plastic hinge formation in the region, and avoid the brittle fracture of beam end welds. In contrast, the proposed joint with RBS region exhibited better ductility and energy dissipation capability than the joint with untreated steel beam. The energy dissipation of the specimens was affected significantly by the bond slip after the anchoring bars entered the yield stage. Therefore, When the anchorage length of the bars satisfies the design recommendations, a sufficient reinforcement ratio of the bars should be ensured to prevent the emergence of the anchorage bar bond slippage after the bars yielded.