生物墨水挤出打印成型精度评价方法概述

doi:10.3785/j.issn.1008-973X.2023.04.001

浙江大学学报(工学版)

2023, Vol. 57

Issue (4): 643-656 DOI: 10.3785/j.issn.1008-973X.2023.04.001

机械与能源工程

生物墨水挤出打印成型精度评价方法概述

林泽宁(

),蒋涛*(

),尚建忠,杨云,洪阳,罗自荣

国防科技大学智能科学学院，湖南长沙 410073

Overview of methods for evaluating accuracy of bioink extrusion bioprinting

Ze-ning LIN(

),Tao JIANG*(

),Jian-zhong SHANG,Yun YANG,Yang HONG,Zi-rong LUO

College of Intelligence Science and Technology, National University of Defense Technology, Changsha 410073, China

全文: PDF(2222 KB) HTML

摘要：

为了推动挤压生物3D打印结构精度的提升，介绍了以水凝胶为代表的生物墨水挤压打印原理及相关数学模型. 针对打印精度的影响因素，从结构设计、生物墨水特性、打印设备及工艺参数三方面进行系统分析，总结各参数对打印精度的作用. 按照定量评价方法所涉及参数的维度归纳总结并分析不同方法的优缺点，从仿真预测、克服材料力学行为、辅助打印等方面提出研究思路，为后续挤压生物3D打印技术的进一步发展提供参考.

关键词： 挤压生物3D打印; 打印精度; 结构设计; 生物墨水特性; 工艺参数; 定量打印评价方法

Abstract:

The principle of extrusion-based bioprinting with bioinks represented by hydrogels and the related mathematical model were introduced in order to improve the accuracy of extrusion-based 3D bioprinting structure. The factors affecting the printing accuracy were systematically analyzed in terms of structural design, bioink properties, bioprinting equipment and technological parameters, and the role of each parameter on the printing accuracy was summarized. The advantages and disadvantages of different methods were analyzed based on the dimensions of the parameters involved in the quantitative evaluation methods. Research ideas from simulation prediction, overcoming material mechanical behaviors and assisted printing were proposed, which provided references for the further development of the extrusion-based 3D bioprinting technology.

Key words: extrusion-based 3D bioprinting printing accuracy structure design bioink property process parameter quantitative printing evaluation method

收稿日期: 2022-08-28 出版日期: 2023-04-21

CLC:

TH 164

基金资助: 国家自然科学基金资助项目（52105039）；湖南省研究生科研创新资助项目（CX20220027）；国防科技大学科研计划资助项目（ZK-19）；国防科技大学智能科学学院青年骨干教师资助项目（4142Z6G2）

通讯作者: 蒋涛 E-mail: linzening@nudt.edu.cn;jiangtao@nudt.edu.cn

作者简介: 林泽宁（1997—），男，博士生，从事生物3D打印与生物机器人的研究. orcid.org/0000-0002-5827-0594. E-mail： linzening@nudt.edu.cn

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引用本文:

林泽宁,蒋涛,尚建忠,杨云,洪阳,罗自荣. 生物墨水挤出打印成型精度评价方法概述[J]. 浙江大学学报(工学版), 2023, 57(4): 643-656.

Ze-ning LIN,Tao JIANG,Jian-zhong SHANG,Yun YANG,Yang HONG,Zi-rong LUO. Overview of methods for evaluating accuracy of bioink extrusion bioprinting. Journal of ZheJiang University (Engineering Science), 2023, 57(4): 643-656.

链接本文:

https://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2023.04.001 或 https://www.zjujournals.com/eng/CN/Y2023/V57/I4/643

图 1 挤压生物3D打印过程

图 2 3种挤出生物3D打印技术

表 1 挤压生物3D打印参数及其效果

图 3 生物墨水的流变特性

图 4 不同的打印参数测试

图 5 实体模型结构设计所需考虑的因素

图 6 悬浮打印的三维结构

图 7 流体仿真与机器学习的测试结果

图 8 一维定量评价方法

图 9 基于面积变化的评价方法

图 10 塌陷度二维评价方法

表 2 不同维度的定量评价方法比较

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