Analysis of structures and expression patterns of the flavin-containing monooxygenase family genes in <i>Bursaphelenchus xylophilus</i>

doi:10.3785/j.issn.1008-9209.2022.03.021

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (2): 191-199 DOI: 10.3785/j.issn.1008-9209.2022.03.021

Special Topic: Insect Physiology and Biochemistry & Pest Biological Control

Analysis of structures and expression patterns of the flavin-containing monooxygenase family genes in Bursaphelenchus xylophilus

Xin HAO¹(

),Ruina TAN²,Jie CHEN¹,Yang LI³,Jingxin CAO¹,Jian DIAO¹,Zhen DENG²,Ping ZHANG⁴,Ling MA^1,²(

)

^1.School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China
^2.Innovation Center for Forest Protection Technology of Heilongjiang Province, Northeast Forestry University, Harbin 150040, Heilongjiang, China
^3.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
^4.Heilongjiang Provincial Key Laboratory of Forest Sustainable Management and Environmental Microbiology Engineering, Northeast Forestry University, Harbin 150040, Heilongjiang, China

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Abstract

Flavin-containing monooxygenases (FMOs) participate in endogenous and exogenous metabolisms in organisms, and exist widely in plants, animals and microorganisms. To explore the function of Bursaphelenchus xylophilus FMOs (BxFMOs) in the host colonization and in response to the nematicide stress, we firstly screened and identified 13 Bxfmos from the genome of B. xylophilus by means of bioinformatics, and then analyzed the physicochemical properties, evolutionary development, protein structures, and gene expression patterns of these genes. The results showed that, the Bxfmos distributed on the five chromosomes; their numbers of amino acids were from 432 to 572; their molecular weights were between 49.77 kDa and 66.09 kDa; and their isoelectric points were between 6.26 and 9.27. The structures of BxFMOs were relatively conservative. The gene expression patterns revealed that the BxFMOs had significant effects on the colonization of B. xylophilus and its respondence to nematicidal agents. The above results have important guiding significance and theoretical value for exploring the colonization mechanism of B. xylophilus in the host and seeking the molecular targets to control B. xylophilus.

Key words： Bursaphelenchus xylophilus flavin-containing monooxygenase family genes protein structure bioinformatics

Received: 02 March 2022 Published: 27 April 2023

CLC:

S763.49

Corresponding Authors: Ling MA E-mail: xinhao@nefu.edu.cn;maling63@163.com

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	Xin HAO
	Ruina TAN
	Jie CHEN
	Yang LI
	Jingxin CAO
	Jian DIAO
	Zhen DENG
	Ping ZHANG
	Ling MA

Cite this article:

Xin HAO,Ruina TAN,Jie CHEN,Yang LI,Jingxin CAO,Jian DIAO,Zhen DENG,Ping ZHANG,Ling MA. Analysis of structures and expression patterns of the flavin-containing monooxygenase family genes in Bursaphelenchus xylophilus. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 191-199.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.03.021 OR https://www.zjujournals.com/agr/Y2023/V49/I2/191

松材线虫黄素单加氧酶家族基因结构与表达模式分析

黄素单加氧酶（flavin-containing monooxygenases, FMOs）是一类广泛存在于植物、动物、微生物中，主要参与调控生物体对内源性及外源性物质代谢的生物酶家族。为探究松材线虫FMOs（Bursaphelenchus xylophilus FMOs, BxFMOs）在宿主中定殖和响应杀线剂胁迫过程中所发挥的功能，本研究首先通过生物信息学手段从松材线虫基因组中筛选并鉴定了13个BxFMOs家族基因，随后对获得的BxFMOs家族基因进行理化特性、进化发育、蛋白结构及基因表达模式分析。结果表明：BxFMOs家族成员分布于5条染色体上，氨基酸数目为432～572个，分子量为49.77～66.09 kDa，等电点为6.26～9.27，其结构较为保守。基因表达模式分析揭示，BxFMOs家族对松材线虫定殖和响应杀线剂胁迫具有显著影响。本研究对探索松材线虫在宿主中的定殖机制和寻求防治松材线虫分子靶标具有重要的指导意义和理论价值。

关键词： 松材线虫, 黄素单加氧酶家族基因, 蛋白结构, 生物信息学

Table 1 Analysis of the physicochemical properties of BxFMOs

Fig. 1 Phylogenetic tree of BxFMOs and FMOs of other nematodesCe: Caenorhabditis elegans; Cre: Caenorhabditis remanei.

Fig. 2 Gene structures of BxFMOs

Fig. 3 Conserved motif structures of BxFMOs

Fig. 4 Chromosomal localization of BxFMOs

Table 2 Structural analysis of BxFMOs

Fig. 5 Predicted three-dimensional structures of BxFMOs

Fig. 6 Expression profiles of BxFMOs after Bursaphelenchus xylophilus inoculation to Pinus thunbergii seedlings or under the rotenone stress


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