| Biological sciences & biotechnology |
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| Bioinformatics analysis of duck PPARα gene structure and function |
| MA Yun１ , WANG Yun-yun１ ,２ , ZHANG Xiao-ting１ , LI Fen１ , WANG Qi-zhao１ ,３ ,WANG Xin-zhuang４ |
| 1 . College of Life Sciences , Xinyang Normal University , Xinyang , Henan 464000 , China ;2 . School o f L i f e Science and Technology , Huazhong University of Science & Technology ,Wuhan 430074 , China ;3 . Institute of Nuclear Agricultural Science , Zhej iang University , Hangzhou 310029 ,China ;4 . College of Animal Husbandry and Veterinary Medicine ,Henan Agricultural University ,Zhengzhou 450002 ,China |
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Abstract Protein structures , physical and chemical properties , and their functional domain structures encoded by duck peroxisome proliferators‐activated receptors‐α ( PPARα ) gene were analyzed using bioinformatics method . Sequencing and bioinformatics analysis showed that the duck PPA Rα cDNA sequence was １ ４３０ base pairs long with an open reading frame ( ORF) containing １ ４０７ nucleotides encoding a protein of ４６８ amino acids . The multiple sequence alignment showed that coding nucleotide sequences of the obtained duck PPA Rα gene had ９６％ , ９２％ , ７９％ , ７７％ , ７８％ homology with the corresponding chicken , T aeniopygia guttata , human , bovine and Equus caballus , respectively , and the deduced amino acid sequences had ９８％ , ９７％ , ８９％ , ８９％ , ８８％ identity with the corresponding species , respectively . Phylogenetic tree analysis of the amino acid sequences of duck PPAR α and other PPARα proteins in human and other animals showed that , among all the species mentioned above , the nearest relationship existed between duck and chicken . Further analysis on the structure and function of the duck PPARα protein showed that there were much more α helix than other secondary structure in the duck PPARα protein , besides , it had a short nuclear localization signal (KKNRNKC) , so that it could enter into nucleus . Moreover , a DNA binding domain which was composed of two C４‐type zinc fingers and a ligand binding domain was found in the PPARα protein .
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Published: 20 July 2011
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鸭PPARα基因结构及功能的生物信息学分析
以鸭的过氧化物酶体增殖物激活受体α( PPARα)基因为研究对象,运用生物信息学方法对其编码的蛋白质结构、理化性质及功能结构域进行分析.结果表明:鸭的PPA Rα 基因cDNA 全长１ ４３０ bp ,最长开放阅读框为１ ４０７ bp ,编码４６８ 个氨基酸;序列比对结果显示鸭的PPA Rα 基因与鸡、胸草雀、人、牛、家马等该基因的核苷酸序列同源性分别为９６％ 、９２％ 、７９％ 、７７％ 和７８％ ,鸭PPA Rα 基因编码的氨基酸序列与上述其他动物的同源性高达９８％ 、９７％ 、８９％ 、８９％ 和８８％ ;系统进化分析结果显示,在鸡、胸草雀、人、牛、马等动物中,鸭的PPA Rα 基因与鸡的进化关系最为密切,亲缘关系最近;鸭的PPARα 蛋白中α 螺旋较多,该蛋白质含有１ 段短的核定位信号序列KKNRNKC ,在引导该蛋白质进入细胞核的过程中发挥作用,同时还有由２ 个锌指结构组成的DNA 结合域及１ 段配体结合域.
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