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| Single nucleotide polymorphism screening and bioinformatics analysis of myostatin gene in Jingning chicken |
| ZHANG Li1*, LIU Lixia1, DAI Hongwei1, CHEN Hong1, WANG Rui1, YUE Binghui2 |
(1. College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China; 2. Qinghai Vocational and
Technical Institute of Animal Husbandry and Veterinary, Huangyuan 812100, Qinghai, China)
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Abstract In order to screen out the individuals of myostatin (MSTN) gene mutation, and breed fine poultry breeds with higher meat yield through a number of generations in Jingning chicken population, the fifth generation of Jingning chicken was selected as experimental objects to construct DNA pools, and the MSTN gene encoding sequence of Jingning chicken was obtained through segment amplification method, and the single nucleotide polymorphism (SNP) screening and bioinformatics analysis were carried out. The results showed that the coding sequence (CDS) region of MSTN gene in Jingning chicken was 1 128 bp long, encoding 375 amino acids, and the protein encoded by MSTN gene was hydrophilic protein. Four SNPs were found in CDS region including exon1-c.60G＞A, exon1-c.195C＞G, exon1-c.234G＞A, exon3-c.966G＞T, which were all synonymous mutations. The change of mRNA, secondary structure and free energy after nucleotide mutation led to the change of its structural stability. The main components of the protein secondary structure and tertiary structure were random coil, and the extended strand, α -helix and β -turn were scattered throughout the protein structure. The above result indicates that the MSTN gene of Jingning chicken has high mutagenicity, which provides molecular basis for marker-assisted selection of growth traits in Jingning chicken.
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Published: 30 October 2018
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静宁鸡肌肉生长抑制素基因单核苷酸多态性筛查及生物信息学分析
为了在静宁鸡群体中筛选出肌肉生长抑制素基因（myostatin, MSTN）突变个体，通过若干世代选育而培育出产肉率较高的优良家禽品种，以第5 世代静宁鸡为试验对象构建DNA混合池，通过分段扩增获得静宁鸡MSTN基因编码序列，并对其进行单核苷酸多态性（single nucleotide polymorphism, SNP）筛查和生物信息学分析。结果表明：静宁鸡MSTN基因编码区全长1 128 bp，编码375 个氨基酸，该蛋白为亲水性蛋白；编码区共筛查到4 个SNPs，分别为exon1-c.60G＞A、exon1-c.195C＞G、exon1-c.234G＞A、exon3-c.966G＞T，均为同义突变；核苷酸突变后mRNA二级结构和自由能的改变导致其结构稳定性发生改变；蛋白质二、三级结构的主要元件为无规则卷曲，延伸链、α-螺旋和β-转角则散布于整个蛋白质结构中。综上表明，静宁鸡MSTN基因具有较高的突变性，为静宁鸡生长性状的标记辅助选择提供了分子基础。
关键词：
静宁鸡,
肌肉生长抑制素基因,
单核苷酸多态性,
生物信息学
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