Sequencing and analysis of the complete mitochondrial genome of <i>Endoclita minanus</i> Yang(Lepidoptera: Hepialidae)

doi:10.3785/j.issn.1008-9209.2022.03.282

Journal of Zhejiang University (Agriculture and Life Sciences)

2023, Vol. 49

Issue (2): 179-190 DOI: 10.3785/j.issn.1008-9209.2022.03.282

Special Topic: Insect Physiology and Biochemistry & Pest Biological Control

Sequencing and analysis of the complete mitochondrial genome of Endoclita minanus Yang(Lepidoptera: Hepialidae)

Yan LI¹(

),Zhihong LI¹,Wei ZHANG¹(

),Shouke ZHANG²,Jinping SHU¹

^1.Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China
^2.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China

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Abstract

In this study, we explored the taxonomic status of Endoclita minanus Yangin Hepialidae based on the mitochondrial genome level. The complete mitochondrial genome of Endoclita minanus was sequenced by using Illumina MiSeq sequencing platform, and its general features and base composition were analyzed. The phylogenetic tree of mitochondrial genomes of nine species in Hepialidae was constructed by using maximum likelihood method, and their phylogenetic relationships within Hepialidae were analyzed. The results showed that the mitochondrial genome of Endoclita minanus is a circular molecule of 15 248 bp, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and an A+T-rich region with a typical gene arrangement of the mitochondrial genome in Lepidoptera. The A+T content is 81.18%. The gene order of trnI-trnQ-trnM in the mitochondrial genome of Endoclita minanus is consistent with the other species of Hepialidae, which is different from the gene order of trnM-trnI-trnQ in all previously sequenced species of Lepidoptera. The phylogenetic relationships of nine species within Hepialidae based on mitochondrial genomes present as Ahamus+[Napialus+(Endoclita+Thitarodes)]. Gene rearrangement existed in the mitochondrial genome of Endoclita minanus. The phylogenetic analyses strongly supported that Endoclita minanus and Endoclitasignifer gathered into one clade. This study provides an informative reference for understanding the evolution laws of Hepialidae and lays a certain theoretical basis for exploring the phylogeny and evolution of Lepidoptera.

Key words： Endoclita minanus Yang mitochondrial genome phylogenetic relationship gene rearrangement

Received: 28 March 2022 Published: 27 April 2023

CLC:

Q951.3

Corresponding Authors: Wei ZHANG E-mail: ljy0926141319@163.com;zwlzhi@126.com

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Cite this article:

Yan LI,Zhihong LI,Wei ZHANG,Shouke ZHANG,Jinping SHU. Sequencing and analysis of the complete mitochondrial genome of Endoclita minanus Yang(Lepidoptera: Hepialidae). Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 179-190.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.03.282 OR https://www.zjujournals.com/agr/Y2023/V49/I2/179

闽鸠蝙蛾（鳞翅目：蝙蝠蛾科）线粒体基因组全序列测定和分析

本研究旨在从线粒体基因组水平探讨闽鸠蝙蛾（Endoclita minanus Yang）在蝙蝠蛾科（Hepialidae）内的分类地位。通过Illumina MiSeq平台测序技术测定闽鸠蝙蛾线粒体基因组全序列，分析其结构特点和碱基组成；使用最大似然法构建蝙蝠蛾科9种昆虫线粒体基因组的系统发育树，分析其在蝙蝠蛾科内的系统发育关系。结果显示：闽鸠蝙蛾线粒体基因组全长15 248 bp，包含13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和一段典型的鳞翅目昆虫线粒体基因组基因排列的A+T富含区，且A+T含量为81.18%。闽鸠蝙蛾的线粒体基因组排列顺序为trnI-trnQ-trnM，与其他蝙蝠蛾科昆虫顺序相同，而与鳞翅目其他昆虫的线粒体基因组排列顺序（trnM-trnI-trnQ）不同。基于线粒体基因组分析，得到蝙蝠蛾科9种昆虫的系统发育关系为无钩蝠蛾属（Ahamus）+{棒蝠蛾属（Napialus）+［蝙蝠蛾属（Endoclita）+钩蝠蛾属（Thitarodes）］}。闽鸠蝙蛾线粒体基因组存在基因重排现象，系统发育分析支持闽鸠蝙蛾和桉蝙蛾聚为一支。本研究为掌握蝙蝠蛾科昆虫的进化规律提供了信息参考，为探究鳞翅目昆虫的系统发育和进化提供了一定的理论依据。

关键词： 闽鸠蝙蛾, 线粒体基因组, 系统发育关系, 基因重排

Table 1 Information of insect species used for mitochondrial genome analysis

Fig. 1 Structure of the mitochondrial genome of Endoclita minanus

Table 2 Organization of the mitochondrial genome of Endoclita minanus

Table 3 Base compositions of the mitochondrial genome, PCGs, and rRNA genes of Endoclita minanus

Fig. 2 Percentages of amino acids in proteins coded by PCGs of the mitochondrial genome of Endoclita minanus

Table 4 Frequency of utilization and RSCU of amino acids encoded by PCGs of the mitochondrial genome in Endoclita minanus

Fig. 3 Comparison of the secondary structures of tRNA genes between Endoclita minanus and Endoclita signifer

Fig. 4 Phylogenetic tree of 13 PCGs sequences of nine species of Hepialidae based on maximum likelihood methodBootstrap support value (maximum likelihood≥50%) and Bayesian posterior probability value≥0.80 are shown at the nodes.


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