甘蓝型油菜隐性细胞核雄性不育系LY31AB的选育及利用

doi:10.3785/j.issn.1008-9209.2023.03.291

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (4): 507-515 DOI: 10.3785/j.issn.1008-9209.2023.03.291

研究论文

甘蓝型油菜隐性细胞核雄性不育系LY31AB的选育及利用

许明超¹(

),黄倩²,张康妮²,邹旭东¹,陈兴凡¹,陆鸿¹,王艳惠¹(

),张志¹(

)

^1.乐山市农业科学研究院，四川乐山 614000
^2.浙江大学农业与生物技术学院作物科学研究所，浙江杭州 310058

Breeding and utilization of recessive genic male sterile line LY31AB in Brassica napus L.

Mingchao XU¹(

),Qian HUANG²,Kangni ZHANG²,Xudong ZOU¹,Xingfan CHEN¹,Hong LU¹,Yanhui WANG¹(

),Zhi ZHANG¹(

)

^1.Leshan Academy of Agricultural Sciences, Leshan 614000, Sichuan, China
^2.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China

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摘要：

为充分利用细胞核雄性不育的优势，以甘蓝型油菜‘Q1012’商品油菜籽为来源，用系谱选育法选育出双低优质甘蓝型油菜隐性细胞核雄性不育系LY31AB，分析其育性、抗性、配合力等特点，并进行杂种优势利用。结果表明：LY31AB不育性遗传稳定，受2对隐性重叠基因控制，且在田间表现出良好的菌核病抗性和抗倒性；LY31AB配合力高、恢复源广、杂种优势强，其所配组合大多表现出明显的杂种优势；利用LY31AB育成并登记的乐油8号、得乐油1632、万家油520、乐油7号4个双低优质油菜新品种也均表现出菌核病抗性和良好的抗倒性，已被大面积推广应用。综上所述，LY31AB是一个选育系谱清楚、遗传性状稳定、品质优、丰产性好、抗性好、配合力高、恢复源广且具有良好杂种优势利用价值的不育系，有着广阔的应用前景。

关键词： 细胞核雄性不育系; 选育; 育性; 抗性; 配合力; 杂种优势利用

Abstract:

In order to make full use of the advantages of genic male sterility (GMS), the commercial rapeseed ‘Q1012’ of Brassica napus L. was used as the source to select the double-low and high-quality recessive GMS line LY31AB of B. napus by the pedigree breeding method. LY31AB fertility, resistance, combining ability and other characteristics were analyzed and its heterosis was utilized. The results indicated that the sterility of LY31AB was genetically stable, which was controlled by two pairs of recessive overlapping genes, and showed good resistance to sclerotiniadisease and lodging resistance in the field. LY31AB had high combining ability, wide recovery sources and strong heterosis, and most of its combinations showed obvious heterosis. Four new double-low and high-quality rape varieties, Leyou 8, Deleyou 1632, Wanjiayou 520 and Leyou 7, which were bred using LY31AB and registered, also showed good resistance tosclerotiniadisease and lodging resistance, and were widely in promotion and application. To sum up, LY31AB is a sterile line with clear pedigree, stable genetic characters, good quality, good fertility, good resistance, high combining ability, wide recovery sources and good heterosis utilization value, and has broad application prospects.

Key words: genic male sterile line breeding fertility resistance combining ability heterosis utilization

收稿日期: 2023-03-29 出版日期: 2023-08-25

CLC:

S565.4

基金资助: 四川省重点研发项目“突破性油料育种材料和方法创新及新品种选育”(2021YFYZ0018);国家现代农业产业技术体系四川省油菜创新团队建设项目;四川省科学技术厅农业科技成果转化项目“宜机收优质高产油菜新品种‘乐油8号’及其配套高产高效栽培技术的推广示范”(2022NZZJ0018)

通讯作者: 王艳惠,张志 E-mail: 1048515670@qq.com;103434183@qq.com;85943012@qq.com

作者简介: 许明超（https://orcid.org/0009-0003-4424-8649），E-mail：1048515670@qq.com

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引用本文:

许明超,黄倩,张康妮,邹旭东,陈兴凡,陆鸿,王艳惠,张志. 甘蓝型油菜隐性细胞核雄性不育系LY31AB的选育及利用[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(4): 507-515.

Mingchao XU,Qian HUANG,Kangni ZHANG,Xudong ZOU,Xingfan CHEN,Hong LU,Yanhui WANG,Zhi ZHANG. Breeding and utilization of recessive genic male sterile line LY31AB in Brassica napus L.. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(4): 507-515.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.03.291 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I4/507

表1 基于不完成双列杂交（NCⅡ）的20种配合力组合设计

图1 LY31AB花器官形态A.不育株花蕾；B.可育株花蕾；C.不育株花；D.可育株花。

图2 在6 000株/667 m2 栽培密度下LY31AB成熟植株形态A.不育株；B.可育株。

表2 LY31AB主要农艺性状表现

表3 2013—2015年LY31AB花期育性表现

表4 LY31AB与4个自交系杂交后代育性情况

表5 LY31AB田间抗性表现

表6 LY31AB育成品种在四川省级油菜新品种联合试验中的表现

表7 LY31AB育成品种品质检测和抗性鉴定结果

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