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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2014, Vol. 40 Issue (1): 25-32    DOI: 10.3785/j.issn.1008-9209.2013.05.233
农业科学     
外源乙烯调控大豆花粉育性的研究
程云清1*, 张奇2， 刘剑锋1， 张会弟1， 张春吉1
（1.吉林师范大学生命科学学院,吉林 四平136000;2.南京农业大学生命科学学院，江苏 南京210095）
Studies on pollen fertility regulated by exogenous ethylene in soybean
Cheng Yunqing1*, Zhang Qi2,  Liu Jianfeng1， Zhang Huidi1, Zhang Chunji1
(1. College of Life Sciences, Jilin Normal University, Siping， Jilin 136000, China; 2. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)
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摘要： 使用质量浓度为400 mg/L的乙烯利水剂对大豆进行喷施处理，探讨乙烯对大豆花粉育性的调节作用。结果表明，外源乙烯处理后，大豆植株的成花量与花粉数量极显著低于对照，而花粉败育率极显著高于对照，外源乙烯处理对大豆花粉育性的抑制作用十分明显；乙烯利与对照处理基因的差异表达谱及实时定量PCR研究结果表明，细胞应答饥饿、花粉管生长、花粉外壁形成等多个大豆花粉发育相关基因的表达出现显著差异；相同时间段相比，乙烯处理的花芽组织中可溶性糖、可溶性淀粉、超氧化物歧化酶与过氧化物酶活性显著低于对照，而丙二醛含量显著高于对照。本研究为外源乙烯制剂调控大豆育性研究提供了理论依据。
Abstract: Heterosis is a very important genetic phenomenon, and  its application  can significantly improve yields and enhance resistance in many crops. The use of this important tool, however,  is limited in soybean production because: 1) The small size of soybean flowers makes it difficult and expensive to emasculate and hybridize；  2) There are only a limited   number of male sterile lines available for hybrid seed production. Therefore, illuminating the mechanisms of male sterile in  soybeans will benefit  heterosis research of soybean.
Previous studies showed that ethylene biosynthesis in the soybean flower was closely related with male sterility.  However, the mechanisms that  ethylene induced male sterility of soybean remains unknown.  Thus,exogenous ethylene treatment was carried out to study its fertility regulation role in soybean. Scanning electron microscopy (SEM) and palynology methods were used to study the effects of ethylene treatment, and real time quantitative PCR (RT-qPCR) was used to evaluate gene expression.
The results showed that flower numbers and pollen amount were  significantly decreased, and abortion ratio of pollen was  significantly promoted in the ethylene treatment compared with the control. The results of difference gene expression profile and RT-qPCR of the control and exogenous ethylene treatments indicated that the expression of eight genes which are related to  cellular response to starvation, pollen growth  and pollen wall assembly was  changed significantly in the ethylene treatment compared with the control. Soluble sugar and starch content, activities of superoxide dismutase and peroxidase were all significantly lower while malondialdehyde content was significantly higher in flower tissue after ethephon treatment than the control.
In conclusion, it is  confirmed that exogenous ethylene treatment will cause male sterility in soybeans, and the expression of eight  genes responsible  for pollen development has been significantly changed after ethylene treatment. Carbon starvation and proactive enzyme injury are involved in the process of male sterility formation. The study above provides a theoretical  basis for future  research on pollen  sterility regulated by  exogenous ethylene in soybean.
出版日期: 2014-01-20
CLC:  Q 948.11  
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程云清1*
张奇2
刘剑锋1
张会弟1
张春吉1

引用本文:

程云清1*, 张奇2， 刘剑锋1， 张会弟1， 张春吉1. 外源乙烯调控大豆花粉育性的研究[J]. 浙江大学学报(农业与生命科学版), 2014, 40(1): 25-32.

Cheng Yunqing1*, Zhang Qi2, Liu Jianfeng1， Zhang Huidi1, Zhang Chunji1. Studies on pollen fertility regulated by exogenous ethylene in soybean. Journal of Zhejiang University (Agriculture and Life Sciences), 2014, 40(1): 25-32.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2013.05.233    或    http://www.zjujournals.com/agr/CN/Y2014/V40/I1/25

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