利用微计算机断层扫描技术重建褐飞虱内部结构

doi:10.3785/j.issn.1008-9209.2022.07.181

浙江大学学报（农业与生命科学版）

2022, Vol. 48

Issue (6): 797-806 DOI: 10.3785/j.issn.1008-9209.2022.07.181

研究论文

利用微计算机断层扫描技术重建褐飞虱内部结构

舒润国¹(

),周行¹,曹子雄²,贺康¹,李飞¹(

)

^1.浙江大学农业与生物技术学院昆虫科学研究所, 浙江省作物病虫生物学重点实验室, 杭州 310058
^2.Object Research Systems(ORS)公司, 加拿大蒙特利尔 (魁北克) H3B 1A7

Reconstruction of internal structures of Nilaparvata lugens using micro-computer tomography technology (Micro CT)

Runguo SHU¹(

),Hang ZHOU¹,Zixiong CAO²,Kang HE¹,Fei LI¹(

)

^1.Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
^2.Object Research Systems (ORS) Inc. , Montr al (Qu bec) H3B 1A7, Canada

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摘要：

褐飞虱（Nilaparvata lugens）是一种重要的水稻害虫。本研究利用微计算机断层扫描技术（micro-computer tomography technology, Micro CT）获得了褐飞虱成虫的断层扫描图，通过人工建模和深度学习相结合的方式对褐飞虱内部组织和器官进行三维建模，获得了褐飞虱中枢神经系统、肌肉组织、消化道和生殖系统的三维模型，保留了这些结构的原始形态，准确地还原了褐飞虱体内各组织和器官的空间排布，并利用Dragonfly软件对昆虫的内部组织进行了空间体积的测量分析。本研究建立并完善了昆虫组织和器官的三维建模技术，有助于更为精准地观察昆虫的内部组织结构，可用于昆虫形态和器官发育的表型观察，为昆虫发育和基因功能研究提供了新技术和新方法。

关键词： 微计算机断层扫描技术; 褐飞虱; 内部结构; 三维重构; 表型组学

Abstract:

The brown planthopper (Nilaparvata lugens) is an important rice pest. In this study, the tomographic images of adult brown planthopper were obtained by using micro-computer tomography technology (Micro CT). Three-dimensional models of internal tissues and organs of the brown planthopper were established by combining manual modeling and deep learning. Three-dimensional models of the central nervous system, muscle tissue, alimentary canal and reproductive system of the brown planthopper were obtained. These models preserved the original morphologies of these structures and accurately restored the spatial arrangement of all tissues and organs within the brown planthopper. Measurements of the internal organization of insects were analyzed using Dragonfly software. This study established and refined the three-dimensional reconstruction technique of insect tissues and organs, which can contribute to a more precise view of the internal organization structure of insects and can be used for phenotypic observation of insect morphology and organ development. It provides a new technique for insect development investigation and gene function analysis in entomology.

Key words: micro-computer tomography technology (Micro CT) brown planthopper internal structure three-dimensional reconstruction phenomics

收稿日期: 2022-07-18 出版日期: 2022-12-27

CLC:

Q 964

基金资助: 国家重点研发计划项目(2021YFD1400100);国家自然科学基金项目(31972354)

通讯作者: 李飞 E-mail: runguoshu@zju.edu.cn;lifei18@zju.edu.cn

作者简介: 舒润国（https://orcid.org/0000-0001-8810-2666），E-mail：runguoshu@zju.edu.cn

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引用本文:

舒润国,周行,曹子雄,贺康,李飞. 利用微计算机断层扫描技术重建褐飞虱内部结构[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(6): 797-806.

Runguo SHU,Hang ZHOU,Zixiong CAO,Kang HE,Fei LI. Reconstruction of internal structures of Nilaparvata lugens using micro-computer tomography technology (Micro CT). Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(6): 797-806.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.07.181 或 https://www.zjujournals.com/agr/CN/Y2022/V48/I6/797

表1 SkyScan 1272 CMOS技术参数

图1 Micro CT内、外部结构A. 上样装置；B. SkyScan 1272高分辨率三维X射线显微镜；C. 成像路径示意图。

图2 原始Micro CT图像的三维建模流程

图3 样本姿态矫正前（A1～A3 ）和姿态矫正后（B1～B3 ）的Micro CT图像X-Y、X-Z、Y-Z代表Micro CT数据的3个平面。

图4 有效信号放大前（A）和有效信号放大后（B）的灰度密度直方图

图5 训练数据的相关位置信息

表2 训练数据相关的体素信息

表3 深度学习模型训练参数

图6 深度学习模型对应的损失函数曲线（A）和监控图（B）

图7 手工修复与立体模型优化阶段的肌肉模型输出结果A.深度学习模型分割的肌肉ROI（绿色）；B.经过手工修复的ROI（紫色）；C.平滑后的ROI（橙色）；D.最终的肌肉网格模型（黄褐色）。

图8 褐飞虱内部结构三维模型

表4 褐飞虱三维模型结构的体积

表5 使用不同三维重构系统的成本对比

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