无抗日粮中添加生物发酵饲料对肉鸡生长性能、养分代谢及血清指标的影响

doi:10.3785/j.issn.1008-9209.2020.11.051

浙江大学学报（农业与生命科学版）

2021, Vol. 47

Issue (5): 628-636 DOI: 10.3785/j.issn.1008-9209.2020.11.051

动物科学与动物医学

无抗日粮中添加生物发酵饲料对肉鸡生长性能、养分代谢及血清指标的影响

姜莱^1,²(

),黄煜博²,袁纯纯²,丁小青²,侯艳彬²,范京辉³,钱利纯^1,²(

)

^1.海南浙江大学研究院，海南三亚 572025
^2.浙江大学动物科学学院，农业农村部华东动物营养与饲料重点实验室，杭州 310058
^3.杭州市农业科学研究院畜牧研究所，杭州 310021

Effects of biofermentation feed added in antibiotic-free diet on growth performance, nutrient metabolism and serum indexes of broiler chickens

Lai JIANG^1,²(

),Yubo HUANG²,Chunchun YUAN²,Xiaoqing DING²,Yanbin HOU²,Jinghui FAN³,Lichun QIAN^1,²(

)

^1.Hainan Institute of Zhejiang University, Sanya 572025, Hainan, China
^2.Key Laboratory of Animal Nutrition and Feed Science of the Ministry of Agriculture and Rural Affairs, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
^3.Animal Husbandry Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310021, China

全文: PDF(887 KB) HTML

摘要：

为研究以茶渣、豆粕和麸皮为底物的发酵饲料对肉鸡生长性能、养分代谢率及血清指标的影响，采用单因素设计，选取240羽体质量相近、健康状况良好的1日龄罗斯308肉鸡，随机分为5组，每组6个重复，每个重复8只鸡，试验期42 d。其中：对照组T₁，饲喂基础日粮；试验组T₂，饲喂基础日粮＋亚甲基水杨酸杆菌肽（40 mg/kg）；试验组T₃、T₄、T₅，分别用发酵饲料代替基础饲粮中2%、4%和6%的豆粕。结果表明：1）与对照组T₁相比，T₄组肉鸡 1—21日龄的平均日增质量、21日龄和42日龄体质量均显著增加（P＜0.05），1—21日龄料重比显著降低（P＜0.05）。2）T₄和T₅组肉鸡粗蛋白的表观代谢率显著高于T₁和T₂组（P＜0.05）。3）与对照组T₁相比，T₂和T₅组血清白蛋白水平显著提高（P＜0.05），T₅组血清抗氧化指标（总超氧化物歧化酶、过氧化氢酶活性和丙二醛含量）均差异显著（P＜0.05）。综上所述，在肉鸡饲粮中添加以茶渣、豆粕和麸皮为底物的发酵饲料可以提高肉鸡的生长性能、屠宰性能、粗蛋白的表观代谢率和血清指标水平，呈现和抗生素相似的促生长效果。

关键词： 发酵饲料; 肉鸡; 抗生素; 生产性能; 表观代谢率

Abstract:

The study focuses on the effects of fermented feed with tea residue, soybean meal and bran as substrates on growth performance, nutrient metabolic rates and serum indexes of broiler chickens. Using single factor test design, 240 one-day-of-age Ross 308 broiler chickens with similar body mass and good health were randomly divided into five groups [control group T_l, fed the basic diet; experimental group T₂, fed the basic diet＋methylene salicylic acid bacitracin (40 mg/kg); experimental groups T₃, T₄, T₅, fed the fermented feed to replace 2%, 4% and 6% soybean meal in the basic diet, respectively] with six replicates per treatment and eight chickens per replicate. The test period was 42 d. The results showed that: 1) Compared with the control group T_l, the average daily gain from 1 to 21 days of age, 21 day-of-age and 42 day-of-age body masses of broiler chickens in the T₄ group significantly increased (P＜0.05), and the feed-to-gain ratio was significantly reduced from 1 to 21 days of age (P＜0.05). 2) The apparent metabolic rates of crude protein of broiler chickens in T₄ and T₅ groups were significantly higher than those in T₁ and T₂ groups (P＜0.05). 3) Compared with the control group T_l, the serum albumin levels of the T₂ and T₅ groups significantly increased (P＜0.05), and the serum antioxidant indexes (total superoxide dismutase, catalase activities, and malondialdehyde content) of the T₅ group were significantly different (P＜0.05). In summary, the addition of fermented feed with tea residue, soybean meal and bran as substrates in the diets can improve broiler chickens’ growth performance, slaughter performance, apparent metabolic rates of crude protein and serum index levels, reflecting growth-promoting effects similar to antibiotics.

Key words: fermented feed broiler chickens antibiotics production performance apparent metabolic rate

收稿日期: 2020-11-05 出版日期: 2021-10-27

CLC:

S 831.5

基金资助: 浙江省科学技术厅科技特派员项目

通讯作者: 钱利纯 E-mail: 779862494@qq.com;lcqian@zju.edu.cn

作者简介: 姜莱（https://orcid.org/0000-0002-1903-4038），E-mail：779862494@qq.com

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	姜莱
	黄煜博
	袁纯纯
	丁小青
	侯艳彬
	范京辉
	钱利纯

引用本文:

姜莱,黄煜博,袁纯纯,丁小青,侯艳彬,范京辉,钱利纯. 无抗日粮中添加生物发酵饲料对肉鸡生长性能、养分代谢及血清指标的影响[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(5): 628-636.

Lai JIANG,Yubo HUANG,Chunchun YUAN,Xiaoqing DING,Yanbin HOU,Jinghui FAN,Lichun QIAN. Effects of biofermentation feed added in antibiotic-free diet on growth performance, nutrient metabolism and serum indexes of broiler chickens. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(5): 628-636.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2020.11.051 或 http://www.zjujournals.com/agr/CN/Y2021/V47/I5/628

表1 基础饲粮组成及营养水平

表2 发酵饲料对肉鸡生长性能的影响

表3 发酵饲料对肉鸡屠宰性能的影响 (%)

表4 发酵饲料对肉鸡免疫器官指数的影响 (g/kg)

表5 发酵饲料对肉鸡养分代谢率的影响 (%)

表6 发酵饲料对肉鸡血清抗氧化能力的影响

表7 发酵饲料对肉鸡血清生化指标的影响

1	孙展英,刘树栋,安文亭,等.不同菌种固态发酵马铃薯渣对其营养价值影响研究.饲料研究,2014(11):12-15. SUN Z Y, LIU S D, AN W T, et al. Effects of solid-state fermentation of potato residue with different strains on its nutritional value. Feed Research, 2014(11):12-15. (in Chinese)
2	SHEN Y Z, WANG H R, RAN T, et al. Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations. Journal of Animal Science, 2018,96(9):3916-3927. DOI:10.1093/jas/sky249 doi: 10.1093/jas/sky249
3	刘志云,钟晓霞,谢跃伟,等.发酵玉米-豆粕型全价饲料对生长猪生长性能、粪便臭味物质和菌群区系的影响.畜牧兽医学报,2018,49(6):1169-1177. DOI:10.11843/j.issn.0366-6964.2018.06.009 LIU Z Y, ZHONG X X, XIE Y W, et al. Effects of fermented corn-soybean complete feed on the growth performance, odor concentration and bacterial community in faeces of growing pigs. Chinese Journal of Animal and Veterinary Sciences, 2018,49(6):1169-1177. (in Chinese with English abstract) doi: 10.11843/j.issn.0366-6964.2018.06.009
4	刘江英,朱建津,蒋蓉,等.多菌种联合发酵饲料对肉鸡抗沙门菌感染能力的影响.浙江农业学报,2019,31(2):229-234. DOI:10.3969/j.issn.1004-1524.2019.02.08 LIU J Y, ZHU J J, JIANG R, et al. Effect of multi-strain co-fermented feed on anti-Salmonella infection in broilers. Acta Agriculturae Zhejiangensis, 2019,31(2):229-234. (in Chinese with English abstract) doi: 10.3969/j.issn.1004-1524.2019.02.08
5	HUEBNER K L, MARTIN J N, WEISSEND C J, et al. Effects of a Saccharomyces cerevisiae fermentation product on liver abscesses, fecal microbiome, and resistome in feedlot cattle raised without antibiotics. Scientific Reports, 2019,9:2559. DOI:10.1038/s41598-019-39181-7 doi: 10.1038/s41598-019-39181-7
6	GāIGA G, ANSONSKA L, VALDOVSKA A. Effect of probiotics and herbal products on intestinal histomorphological and immunological development in piglets. Veterinary Medicine International, 2020(7):3461768. DOI:10.1155/2020/3461768 doi: 10.1155/2020/3461768
7	Association of Official Analytical Chemists (AOAC). Official Methods of Analysis. 18th ed. Washington, U. S.: AOAC, 2007. DOI:10.1002/0471740039.vec0284 doi: 10.1002/0471740039.vec0284
8	LEE H S, KIM M R, PARK Y, et al. Fermenting red ginseng enhances its safety and efficacy as a novel skin care anti-aging ingredient: in vitro and animal study. Journal of Medicinal Food, 2012,15(11):1015-1023. DOI:10.1089/jmf.2012.2187 doi: 10.1089/jmf.2012.2187
9	CHEN K L, KHO W L, YOU S, et al. Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers. Poultry Science, 2009,88(2):309-315. DOI:10.3382/ps.2008-00224 doi: 10.3382/ps.2008-00224
10	WANG J H, GUO H, ZHANG T R, et al. Growth performance and digestion improvement of juvenile sea cucumber Apostichopus japonicus fed by solid-state fermen-tation diet. Aquaculture Nutrition, 2017,23(6):1312-1318. DOI:10.1111/anu.12506 doi: 10.1111/anu.12506
11	SHI C Y, HE J, WANG J P, et al. Effects of Aspergillus niger fermented rapeseed meal on nutrient digestibility, growth performance and serum parameters in growing pigs. Animal Science Journal, 2016,87:557-563. DOI:10.1111/asj.12457 doi: 10.1111/asj.12457
12	ZHANG J Y, BAEK D H, KIM I H. Effect of dietary supplemental medium chain fatty acids instead of antibiotics on the growth performance, digestibility and blood profiles in growing pigs. Journal of Animal Physiology and Animal Nutrition, 2019,103:1946-1951. DOI:10.1111/jpn.13175 doi: 10.1111/jpn.13175
13	崔卫涛,代娟,吴贝,等.联合使用发酵饲料和发酵中草药微生态制剂替代饲用抗生素对蛋鸡的影响与研究.畜牧与饲料科学,2018,39(11):27-30. DOI:10.16003/j.cnki.issn1672-5190.2018.11.006 CUI W T, DAI J, WU B, et al. The effects of replacement of feeding antibiotics with the combination of a fermented feed and probiotics prepared with fermented Chinese herbal medicine on production performance of laying hens. Animal Husbandry and Feed Science, 2018,39(11):27-30. (in Chinese with English abstract) doi: 10.16003/j.cnki.issn1672-5190.2018.11.006
14	VLADIMIR C, VYACHESLAV M, MICHAEL L C, et al. Poultry-beneficial solid-state Bacillus amyloliquefaciens B-1895 fermented soybean formulation. Bioscience of Microbiota Food and Health, 2015,34(1):25-28. DOI:10.12938/bmfh.2014-012 doi: 10.12938/bmfh.2014-012
15	GOODARZI B F, SENZ M, KOZOWSKI K, et al. The effects of fermentation and enzymatic treatment of pea on nutrient digestibility and growth performance of broilers. Animal, 2017,11(10):1698-1707. DOI:10.1017/S1751731117000787 doi: 10.1017/S1751731117000787
16	李阳.解淀粉芽孢杆菌发酵豆粕工艺及其对肉鸡生长影响的机理研究.北京:中国农业科学院,2020. DOI:10.1515/9783110682571 LI Y. Study on fermentation technology of soybean meal by Bacillus amyloliquefaciens and its effect on growth of broiler chickens. Beijing: Chinese Academy of Agricultural Sciences, 2020. (in Chinese with English abstract) doi: 10.1515/9783110682571
17	孙军学,齐国涛.亚麻籽饼粕发酵液对肉鸡生产性能和屠宰性能的影响.甘肃畜牧兽医,2019,49(9):48-52. DOI:10.15979/j.cnki.cn62-1064/s.2019.09.015 SUN J X, QI G T. Effect of flaxseed cake ferment liquid on production and slaughtering performance of broiler chickens. Gansu Animal Husbandry and Veterinary, 2019,49(9):48-52. (in Chinese) doi: 10.15979/j.cnki.cn62-1064/s.2019.09.015
18	李丹,江连洲,娄巍,等.大豆肽对白羽肉鸡生产性能及胴体品质的影响.饲料研究.2009(2):31-34. LI D, JIANG L Z, LOU W, et al. Effect of soybean peptide on production performance and carcass quality of white feather broiler chickens. Feed Research, 2009(2):31-34. (in Chinese)
19	林丽花,谢丽曲,王长康,等.发酵豆粕对1—4周龄黄羽肉鸡生长性能、消化酶活性、抗氧化能力和免疫器官指数的影响.福建农林大学学报(自然科学版),2013,42(4):403-409. DOI:10.13323/j.cnki.j.fafu(nat.sci.).2013.04.021 LIN L H, XIE L Q, WANG C K, et al. Effects of fermented soybean meal on growth performance, digestive enzyme, antioxidant ability and immune organ index of 1-4 weeks old yellow broilers. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2013,42(4):403-409. (in Chinese with English abstract) doi: 10.13323/j.cnki.j.fafu(nat.sci.).2013.04.021
20	柯祥军,瞿明仁,易中华.不同水平发酵豆粕对肉鸡生产性能及营养素表观消化率的影响.饲料工业,2008,29(13):33-36. DOI:10.3969/j.issn.1008-6137.2008.02.004 KE X J, QU M R, YI Z H. Effects of fermented soybean meal at different levels on production performance and nutrient apparent digestibility of broiler chickens. Feed Industry, 2008,29(13):33-36. (in Chinese) doi: 10.3969/j.issn.1008-6137.2008.02.004
21	魏金涛,赵娜,杨雪海,等.发酵饲料对断奶仔猪生产性能、血液生化指标和饲料养分表观消化率的影响.中国粮油学报,2009,24(2):129-133. WEI J T, ZHAO N, YANG X H, et al. Effects of fermented feed on growth performance, plasma biochemical parameters and digestibility of dietary components in weaned piglets. Journal of the Chinese Cereals and Oils Association, 2009,24(2):129-133. (in Chinese with English abstract)
22	杨宏波,刘红,余同水,等.不同添加水平半胱胺对奶牛泌乳性能和血清生化指标的影响.中国农业大学学报,2015,20(5):201-208. DOI:10.11841/j.issn.1007-4333.2015.05.27 YANG H B, LIU H, YU T S, et al. Effects of different cysteamine hydrochloride supplement on milk production and serum biochemical indicators of lactating dairy cows. Journal of China Agricultural University, 2015,20(5):201-208. (in Chinese with English abstract) doi: 10.11841/j.issn.1007-4333.2015.05.27
23	冯建,王萍,何娇娇,等.发酵豆粕替代鱼粉对大黄鱼幼鱼生长性能、体成分、血清生化指标及肝脏组织形态的影响.动物营养学报,2016,28(11):3493-3502. DOI:10.3969/j.issn.1006-267x.2016.11.016 FENG J, WANG P, HE J J, et al. Effects of replacement of fish meal by fermented soybean meal on growth performance, body composition, serum biochemical indices and liver tissue morphology of juvenile large yellow croaker (Larimichthys crocea). Chinese Journal of Animal Nutrition, 2016,28(11):3493-3502. (in Chinese with English abstract) doi: 10.3969/j.issn.1006-267x.2016.11.016
24	YOUSEF M I, KAMEL K I, EAMAIL A M, et al. Antioxidant activities and lipid lowering effects of isoflavone in male rabbits. Food and Chemical Toxicology, 2004,42(9):1497-1503. DOI:10.1016/j.fct.2004.04.012 doi: 10.1016/j.fct.2004.04.012
25	李哲明,何嘉娜,刘琼,等.安吉白茶茶多酚对D-半乳糖致衰老模型小鼠的抗氧化作用研究.贵州中医药大学学报,2016,38(2):21-24. DOI:10.16588/j.cnki.issn1002-1108.2016.02.006 LI Z M, HE J N, LIU Q, et al. The antioxidant effect of polyphenols of Anji white tea on D-galactose induced aging model mice. Journal of Guizhou University of Traditional Chinese Medicine, 2016,38(2):21-24. (in Chinese with English abstract) doi: 10.16588/j.cnki.issn1002-1108.2016.02.006
26	李桦,杨梅梅,屈倩,等.绿茶多酚对热应激肉鸡血生化指标和抗氧化能力的影响.中国兽医学报,2016,36(5):801-803. DOI:10.16303/j.cnki.1005-4545.2016.05.19 LI Y, YANG M M, QU Q, et al. Effect of green tea phenols on blood biochemistry and antioxidant capacity in heat stress broilers. Chinese Journal of Veterinary Science, 2016,36(5):801-803. (in Chinese with English abstract) doi: 10.16303/j.cnki.1005-4545.2016.05.19

[1]	高珊,衡诺,郭勇,陈余,董颖超,冯保芹,齐晓龙,吕学泽. 饲粮中添加生物活性硒对蛋鸡生产性能、蛋品质和蛋黄中硒含量的影响[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(2): 261-267.
[2]	汤志宏,徐宁宁,叶均安. 复合益生菌和酵母培养物对热应激奶牛生产性能、瘤胃发酵和血清抗应激指标的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(5): 611-618.
[3]	王志刚,徐超,李霞辉,林诗宇,杜夏夏,冉崇霖,舒刚. 辣木叶粉对鹌鹑生产性能、免疫功能及血清抗氧化指标的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(2): 243-250.
[4]	侯福银,杨智青,陈长宽,时凯,金崇富,陈应江. 原花青素对肉鸡肠道及屠宰性能、脂质代谢和肉品质的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(1): 119-125.
[5]	潘航, 李肖梁, 方维焕, 乐敏. 美国近20 年主要食源性致病菌的分布及耐药性分析——对我国细菌耐药性监控工作的启示[J]. 浙江大学学报（农业与生命科学版）, 2018, 44(2): 237-246.
[6]	张毓森，叶军，苏建强. 农田生态系统抗生素抗性研究进展与挑战[J]. 浙江大学学报(农业与生命科学版), 2017, 43(6): 691-699.
[7]	赵倩明，朱隆基，詹康，霍永久，赵国琦. 不同粉碎粒度苜蓿草粉对扬州鹅生产性能和血液生化指标的影响[J]. 浙江大学学报(农业与生命科学版), 2017, 43(2): 253-261.
[8]	顾超, 张奇春, 徐晨光. 土壤中以抗生素为单一碳源的抗性细菌[J]. 浙江大学学报(农业与生命科学版), 2016, 42(5): 582-.
[9]	焦阳, 黄文明, 郭海明, 叶均安. 含无患子皂甙的精料对后备牛生产性能和瘤胃发酵的影响[J]. 浙江大学学报(农业与生命科学版), 2015, 41(03): 358-364.
[10]	徐秋桐1, 常跃畅1，章明奎1,2. 浙江省土壤中细菌对抗生素耐药性的生态分布特征*[J]. 浙江大学学报(农业与生命科学版), 2013, 39(5): 537-544.
[11]	叶玉娥，叶明，张蓉，蔡振宇，胡士明，刘彩霞. 芽孢杆菌制剂对艾维茵肉鸡胴体性能和肠道菌群的影响[J]. 浙江大学学报(农业与生命科学版), 2012, 38(3): 305-310.
[12]	汤江武,孙宏,姚晓红,吴逸飞,王新. 芽孢杆菌在肉鸡肠道内的分布及对肠道菌群、消化酶活性的影响[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 319-325.
[13]	唐胜球,董小英,陈建荣,杜雄伟,刘厚森. 谷氨酰胺对岭南黄肉鸡生长性能、养分利用率与消化器官发育的影响[J]. 浙江大学学报(农业与生命科学版), 2011, 37(2): 203-211.
[14]	张习文,占秀安,武如娟,王永侠;. 母种鸡补充不同硒源对后代肉鸡硒沉积、抗氧化指标及生长性能的影响[J]. 浙江大学学报(农业与生命科学版), 2010, 36(5): 554-560.
[15]	章世元, 俞路, 王雅倩, 林显华, 汪益峰. 鸡甲状旁腺素基因质粒对蛋鸡蛋壳质量和激素水平的调控研究[J]. 浙江大学学报(农业与生命科学版), 2009, 35(2): 201-208.

Viewed

Full text

Abstract

Cited

Shared

Discussed